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The orchestrated interaction of transmembrane proteins with other molecules mediates several crucial biological processes. Detergent solubilization may significantly alter or even abolish such hetero‐oligomeric interactions, which makes observing them at high resolution in their native environment technically challenging. Dipolar electron paramagnetic resonance (EPR) techniques such as pulsed electro–electron...
Vermessen von Nukleinsäure‐Strukturen: Gepulste Elektron‐Elektron‐Doppelresonanz‐Spektroskopie wurde zum ersten Mal verwendet, um die Struktur von Nukleinsäuren in Oozyten von Xenopus laevis zu vermessen (siehe Bild). Die in vitro und in Zellen gemessenen Abstände sind gleich, was auf die Existenz von stabilen Gesamtkonformationen der untersuchten haarnadelförmigen RNA und des Neomycin erkennenden...
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