Search results for: Brian D. Sykes

Items from 1 to 20 out of 118 results

article

Feasibility of trifluoromethyl TROSY NMR at high magnetic fields

Brittney A. Klein, Brian D. Sykes

Journal of Biomolecular NMR > 2019 > 73 > 10-11 > 519-523

Insights into the structure and dynamics of large biological systems has been greatly improved by two concurrent NMR approaches: the application of transverse relaxation-optimized spectroscopy (TROSY) techniques in multi-dimensional NMR, especially the methyl-TROSY, and the resurgence of ¹⁹F NMR using trifluoromethyl (CF₃) probes. Herein we investigate the feasibility of combining these approaches...

article

Structures reveal details of small molecule binding to cardiac troponin

Fangze Cai, Monica X. Li, Sandra E. Pineda-Sanabria, Shorena Gelozia, more

Journal of Molecular and Cellular Cardiology > 2016 > 101 > C > 134-144

In cardiac and skeletal muscle, the troponin complex turns muscle contraction on and off in a calcium-dependent manner. Many small molecules are known to bind to the troponin complex to modulate its calcium binding affinity, and this may be useful in a broad range of conditions in which striated muscle function is compromised, such as congestive heart failure. As a tool for developing drugs specific...

chapter

The Evaluation of Isotope Editing and Filtering for Protein—Ligand Interaction Elucidation by Nmr

Ian M. Robertson, Leo Spyracopoulos, Brian D. Sykes

NATO Science for Peace and Security Series B: Physics and Biophysics > Biophysics and the Challenges of Emerging Threats > 101-119

A series of experiments that aid in the structural characterization of protein—ligand complexes by NMR have been assessed. Methods have been established to identify intermolecular NOEs between labeled proteins and unlabeled peptides and/or drug ligands, while omitting signal from intramolecular NOEs within both labeled and unlabeled constituents. The protein—peptide complex chosen to illustrate the...

article

Probing the mechanism of cardiovascular drugs using a covalent levosimendan analog

Sandra E. Pineda-Sanabria, Ian M. Robertson, Yin-Biao Sun, Malcolm Irving, more

Journal of Molecular and Cellular Cardiology > 2016 > 92 > C > 174-184

One approach to improve contraction in the failing heart is the administration of calcium (Ca²⁺) sensitizers. Although it is known that levosimendan and other sensitizers bind to troponin C (cTnC), their in vivo mechanism is not fully understood. Based on levosimendan, we designed a covalent Ca²⁺ sensitizer (i9) that targets C84 of cTnC and exchanged this complex into cardiac muscle. The NMR structure...

article

Structure and Function of the Levosimendan Analog I9 Covalently Bound to Cardiac Troponin C

Sandra Elizabeth Pineda-Sanabria, Ian M. Robertson, Yin-Biao Sun, Malcolm Irving, more

Biophysical Journal > 2016 > 110 > 3 Supplement 1 > 123a

article

The structural and functional effects of the familial hypertrophic cardiomyopathy-linked cardiac troponin C mutation, L29Q

Ian M. Robertson, Ivanka Sevrieva, Monica X. Li, Malcolm Irving, more

Journal of Molecular and Cellular Cardiology > 2015 > 87 > C > 257-269

Familial hypertrophic cardiomyopathy (FHC) is characterized by severe abnormal cardiac muscle growth. The traditional view of disease progression in FHC is that an increase in the Ca²⁺-sensitivity of cardiac muscle contraction ultimately leads to pathogenic myocardial remodeling, though recent studies suggest this may be an oversimplification. For example, FHC may be developed through altered signaling...

article

Computer‐Aided Drug Discovery Approach Finds Calcium Sensitizer of Cardiac Troponin

Steffen Lindert, Monica X. Li, Brian D. Sykes, J. Andrew McCammon

Chemical Biology & Drug Design > 85 > 2 > 99 - 106

In the fight against heart failure, therapeutics that have the ability to increase the contractile power of the heart are urgently needed. One possible route of action to improve heart contractile power is increasing the calcium sensitivity of the thin filament. From a pharmaceutical standpoint, calcium sensitizers have the distinct advantage of not altering cardiomyocyte calcium levels and thus have...

article

Conformation of the critical pH sensitive region of troponin depends upon a single residue in troponin I

Ian M. Robertson, Sandra E. Pineda-Sanabria, Peter C. Holmes, Brian D. Sykes

Archives of Biochemistry and Biophysics > 2014 > 552-553 > Complete > 40-49

The calcium sensitivity of cardiac and skeletal muscle is reduced during cytosolic acidosis, and this inhibition is more pronounced in cardiac muscle. Replacing cardiac troponin I with skeletal troponin I reduces the pH sensitivity of cardiac muscle. This diminished pH sensitivity depends on a single amino acid difference in troponin I: an alanine in cardiac and a histidine in skeletal. Studies suggested...

article

Rewiring AMPK and Mitochondrial Retrograde Signaling for Metabolic Control of Aging and Histone Acetylation in Respiratory-Defective Cells

R. Magnus N. Friis, John Paul Glaves, Tao Huan, Liang Li, more

Cell Reports > 2014 > 7 > 2 > 565-574

Abnormal respiratory metabolism plays a role in numerous human disorders. We find that regulation of overall histone acetylation is perturbed in respiratory-incompetent (ρ ⁰ ) yeast. Because histone acetylation is highly sensitive to acetyl-coenzyme A (acetyl-CoA) availability, we sought interventions that suppress this ρ ⁰ phenotype through reprogramming metabolism. Nutritional intervention...

article

In Vitro and in Situ Structure and Function of the Cardiac Troponin C Familial Hypertrophic Cardiomyopathy-Linked Mutation, L29Q

Ian M. Robertson, Ivanka Sevrieva, Monica X. Li, Malcolm Irving, more

Biophysical Journal > 2014 > 106 > 2 Supplement 1 > 723a-724a

article

The Histidine Button Dictates the Conformation of the pH-Sensitive Region of Troponin I

Sandra E. Pineda-Sanabria, Ian M. Robertson, Peter C. Holmes, Brian D. Sykes

Biophysical Journal > 2014 > 106 > 2 Supplement 1 > 467a

article

Structural and functional insights into the cardiac Na+/H+ exchanger

Brian L. Lee, Brian D. Sykes, Larry Fliegel

Journal of Molecular and Cellular Cardiology > 2013 > 61 > C > 60-67

The NHE1 isoform of the Na⁺/H⁺ exchanger is present in the plasma membrane of the mammalian myocardium where it functions to regulate intracellular pH by exchanging one external Na⁺ for an internal proton. The protein is involved in myocardial ischemia/reperfusion damage and in heart hypertrophy. Topology models and experimental evidence suggest that of the 815 amino acids of the protein, approximately...

article

Characterization of a Cardiac Troponin C-Troponin I Chimera Protein

Sandra E. Pineda-Sanabria, Olivier Julien, Monica X. Li, Brian D. Sykes

Biophysical Journal > 2013 > 104 > 2 Supplement 1 > 482a

article

Structural and functional analysis of extracellular loop 4 of the Nhe1 isoform of the Na+/H+ exchanger

Brian L. Lee, Yongsheng Liu, Xiuju Li, Brian D. Sykes, more

BBA - Biomembranes > 2012 > 1818 > 11 > 2783-2790

The mammalian Na ⁺ /H ⁺ exchanger isoform 1 (NHE1) is a ubiquitously expressed plasma membrane protein. It regulates intracellular pH by removing a single intracellular H ⁺ in exchange for one extracellular Na ⁺ . The membrane domain of NHE1 comprises the 500 N-terminal amino acids and is made of 12 transmembrane segments. The extracellular loops of the transmembrane...

article

A PagP fusion protein system for the expression of intrinsically disordered proteins in Escherichia coli

Peter M. Hwang, Jonathan S. Pan, Brian D. Sykes

Protein Expression and Purification > 2012 > 85 > 1 > 148-151

PagP, a beta-barrel membrane protein found in Gram-negative bacteria, expresses robustly in inclusion bodies when its signal sequence is removed. We have developed a new fusion protein expression system based on PagP and demonstrated its utility in the expression of the unstructured N-terminal region of human cardiac troponin I (residues 1–71). A yield of 100mg fusion protein per liter M9 minimal...

article

NMR Structure of a Double-Transmembrane Fragment from NHE1

Brian L. Lee, Claudia Alves, Larry Fliegel, Brian D. Sykes

Biophysical Journal > 2012 > 102 > 3 Supplement 1 > 521a

article

Structural Investigation of the Acidosis Resistant Cardiac Troponin I Mutant A162H

Sandra E. Pineda-Sanabria, Ian M. Robertson, Monica X. Li, Brian D. Sykes

Biophysical Journal > 2012 > 102 > 3 Supplement 1 > 560a

article

Dynamics of a Troponin Chimera Reproduce the Effects of Calcium on the Troponin Complex

Olivier Julien, Claire N. Allen, Pascal Mercier, Carlos H.I. Ramos, more

Biophysical Journal > 2012 > 102 > 3 Supplement 1 > 230a

article

Tryptophan side chain conformers monitored by NMR and time‐resolved fluorescence spectroscopies

Olivier Julien, Guoli Wang, Abel Jonckheer, Yves Engelborghs, more

Proteins: Structure, Function, and Bioinformatics > 80 > 1 > 239 - 245

We have inserted a tryptophan (F77W) in the core of the regulatory domain of cardiac troponin C (cNTnC), and previously determined the structure of this mutant with and without the cosolvent trifluoroethanol (TFE). Interestingly, the orientations of the indole side chain of the Trp are in opposite directions in the two structures (Julien et al., Protein Sci 2009; 18:1165–1174). Fluorescence decay...

article

Relative and Regional Stabilities of the Hamster, Mouse, Rabbit, and Bovine Prion Proteins toward Urea Unfolding Assessed by Nuclear Magnetic Resonance and Circular Dichroism Spectroscopies

Olivier Julien, Subhrangsu Chatterjee, Trent C. Bjorndahl, Braden Sweeting, more

Biochemistry > 2011 > 50 > 35 > 7536 - 7545

Publication date

Set your own date range

Content availability

Available (116)
None (2)

Publication type

article (117)
book (1)

Keywords

NMR (15)
TROPONIN C (8)
NMR SPECTROSCOPY (5)
NA+/H+ EXCHANGER (4)
TROPONIN (4)
ANTIFREEZE PROTEIN (3)
CATION COORDINATION (3)
INSECT (3)
MEMBRANE PROTEIN (3)
TROPONIN I (3)
URINE (3)
ACIDOSIS (2)
BIOMOLECULAR NMR (2)
CALCIUM SENSITIZER (2)
CARDIOTONIC DRUGS (2)
CHEMOKINE (2)
DRUG DEVELOPMENT (2)
LEVOSIMENDAN (2)
METABOLITE (2)
METABOLOMICS (2)
MYOFILAMENT (2)
NUCLEAR MAGNETIC RESONANCE (2)
PEPTIDE (2)
PH REGULATION (2)
PHOSPHORYLATION (2)
PILIN (2)
SIDE CHAIN DYNAMICS (2)
SOLUTION STRUCTURE (2)
STRUCTURE (2)
THERMAL HYSTERESIS (2)
COILED-COILS; ELECTROSTATIC INTERACTIONS; BURIED SALT BRIDGE;; [ 1 H]-NMR; MOLECULAR MODELING (1)
TWO-DIMENSIONAL 1 H-NMR (1)
15N NMR RELAXATION (1)
PSEUDOMONAS (1)
PSEUDOMONAS AERUGINOSA (1)
(LOCUSTA MIGRATORIA) (1)
15N RELAXATION (1)
19F NMR (1)
ACCURACY (1)
ANTIBODY (1)
APOLIPOPROTEIN (1)
ASSIGNMENT (1)
ASSIGNMENT HOMOLOGY PROGRAMS (1)
ASSIGNMENTS (1)
ASTHMA (1)
ATOM NAMING (1)
AUTOMATED (1)
AUTOMATED SEQUENTIAL ASSIGNMENT (1)
AUTOMATIC (1)
BACKBONE DYNAMICS (1)
BACTERIA (1)
BACTERIUM (1)
BETA-HELIX (1)
BIOLOGICAL NMR (1)
BIOLOGICAL SOLID STATE NMR (1)
BIOMACROMOLECULE ORIENTATION AND DYNAMICS (1)
BIOMAGRESBANK (1)
BIOMARKERS (1)
BURIED SALT BRIDGE (1)
CA2+-SENSITIZER (1)
CALCIUM AFFINITY (1)
CALCIUM SENSITIZERS (1)
CARDIAC (1)
CARDIAC MUSCLE CONTRACTION (1)
CARDIAC TROPONIN ACTIVATOR (1)
CARDIOMYOPATHY MUTATIONS (1)
CATION TRANSPORT (1)
CHEMICAL SHIFT (1)
CHEMICAL SHIFT ANISOTROPY (1)
CHEMICAL SHIFT REFERENCING (1)
CHEMICAL SHIFT TENSOR (1)
CHEMICAL SHIFTS (1)
CONFORMATIONAL ENTROPY (1)
COVALENT MODULATOR (1)
CRYSTAL STRUCTURE (1)
CXC CHEMOKINE RECEPTOR 4 (1)
CXCR4 (1)
CYANOGEN BROMIDE CLEAVAGE (1)
CYSTEINE SCANNING MUTAGENESIS (1)
DATABASE (1)
DEPOSITION (1)
DPC (1)
DRUG DISCOVERY (1)
DRUGS (1)
DYNAMICS (1)
EXPRESSION (1)
EXTRACELLULAR LOOP (1)
FAMILIAL HYPERTROPHIC CARDIOMYOPATHY (1)
FLAT-COIL (1)
FLUORESCENCE (1)
FLUORESCENCE SPECTROSCOPY (1)
FLUORINE (1)
FLUORINE-19 NMR (1)
FLY CASTING (1)
FOLDING (1)
FTICR (1)
FUSION PROTEIN EXPRESSION (1)
H-BONDING INTERACTION (1)
H-BONDS (1)
HEART FAILURE (1)
more

Data set

Elsevier (67)
ACS (29)
Springer (19)
Wiley (3)

Journal

Biochemistry (25)
Biophysical Journal (21)
Journal of Biomolecular NMR (13)
Journal of Molecular Biology (9)
Journal of Molecular and Cellular Cardiology (6)
FEBS Letters (5)
Journal of Magnetic Resonance (5)
BBA - Biomembranes (4)
Metabolomics (3)
Current Opinion in Structural Biology (2)
Journal of the American Chemical Society (2)
Protein Expression and Purification (2)
Proteins: Structure, Function, and Bioinformatics (2)
Structure (2)
Analytical Chemistry (1)
Antiviral Research (1)
Archives of Biochemistry and Biophysics (1)
Biochemical and Biophysical Research Communications (1)
Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism (1)
Cell Reports (1)
Chemical Biology & Drug Design (1)
Folding and Design (1)
International Journal of Mass Spectrometry (1)
Journal of Magnetic Resonance, Series A (1)
Journal of Muscle Research and Cell Motility (1)
Journal of Proteome Research (1)
Journal of The American Society for Mass Spectrometry (1)
Journal of the American Society for Mass Spectrometry (1)
Molecular Cell (1)
The Journal of Allergy and Clinical Immunology (1)
more

INFONA - science communication portal

Search results for: Brian D. Sykes

Feasibility of trifluoromethyl TROSY NMR at high magnetic fields

Structures reveal details of small molecule binding to cardiac troponin

The Evaluation of Isotope Editing and Filtering for Protein—Ligand Interaction Elucidation by Nmr

Probing the mechanism of cardiovascular drugs using a covalent levosimendan analog

Structure and Function of the Levosimendan Analog I9 Covalently Bound to Cardiac Troponin C

The structural and functional effects of the familial hypertrophic cardiomyopathy-linked cardiac troponin C mutation, L29Q

Computer‐Aided Drug Discovery Approach Finds Calcium Sensitizer of Cardiac Troponin

Conformation of the critical pH sensitive region of troponin depends upon a single residue in troponin I

Rewiring AMPK and Mitochondrial Retrograde Signaling for Metabolic Control of Aging and Histone Acetylation in Respiratory-Defective Cells

In Vitro and in Situ Structure and Function of the Cardiac Troponin C Familial Hypertrophic Cardiomyopathy-Linked Mutation, L29Q

The Histidine Button Dictates the Conformation of the pH-Sensitive Region of Troponin I

Structural and functional insights into the cardiac Na+/H+ exchanger

Characterization of a Cardiac Troponin C-Troponin I Chimera Protein

Structural and functional analysis of extracellular loop 4 of the Nhe1 isoform of the Na+/H+ exchanger

A PagP fusion protein system for the expression of intrinsically disordered proteins in Escherichia coli

NMR Structure of a Double-Transmembrane Fragment from NHE1

Structural Investigation of the Acidosis Resistant Cardiac Troponin I Mutant A162H

Dynamics of a Troponin Chimera Reproduce the Effects of Calcium on the Troponin Complex

Tryptophan side chain conformers monitored by NMR and time‐resolved fluorescence spectroscopies

Relative and Regional Stabilities of the Hamster, Mouse, Rabbit, and Bovine Prion Proteins toward Urea Unfolding Assessed by Nuclear Magnetic Resonance and Circular Dichroism Spectroscopies

Filter options

Publication date

Content availability

Publication type

Keywords

Data set

Journal

INFONA - science communication portal

Search results for: Brian D. Sykes

Add recipient

Sending message cancelled

Are you sure you want to cancel sending this message?

Send message

Filter options

Publication date

Date range setting

Set the date range to filter the displayed results. You can set a starting date, ending date or both. You can enter the dates manually or choose them from the calendar.

Content availability

Publication type

Keywords

Data set

Journal

Reporting an error / abuse

Sending the report failed

Accessibility options