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The objective of this study was to characterize the differences in electrophoretic behavior between linear and branched PEG‐conjugated proteins. Human growth hormone and alpha‐lactalbumin modified by linear or branched PEGs with molecular weight of 10 kDa were analyzed by SEC, MALDI‐TOF MS, SDS‐PAGE, and microchip CGE (MCGE). Chromatographic and mass spectrometric differences between the linear and...
The purpose of this study was to evaluate the microchip CGE (MCGE) for the analysis of PEG‐modified granulocyte‐colony stimulating factor (PEG‐G‐CSF) prepared with PEG‐aldehydes. The unmodified and PEG‐modified G‐CSFs were analyzed by Protein 80 and 230 Labchips on the Agilent 2100 Bioanalyzer. The MCGE allowed size‐based separation and quantitation of PEG‐G‐CSF. The Protein 80 Labchip was useful...
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