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A coupled system, consisting of a conventional ion-exchange column and a chiral column, was used for separation of the d- and l-enantiomers as constituents of an amino acid mixture. The ion-exchange column was connected to an amino acid analyser and the eluents necessary for the chiral column were delivered by an HPLC pump. With this on-line system the d- and l-forms of eight of the proteinogenic...
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