Search results for: Wei-Jun Qian

Items from 1 to 20 out of 66 results

article

Discovery of potential pathways for biological conversion of poplar wood into lipids by co-fermentation of Rhodococci strains

Xiaolu Li, Yucai He, Libing Zhang, Zhangyang Xu, more

Biotechnology for Biofuels > 2019 > 12 > 1 > 1-16

Background Biological routes for utilizing both carbohydrates and lignin are important to reach the ultimate goal of bioconversion of full carbon in biomass into biofuels and biochemicals. Recent biotechnology advances have shown promises toward facilitating biological transformation of lignin into lipids. Natural and engineered Rhodococcus strains (e.g., R. opacus PD630, R. jostii RHA1, and R. jostii...

article

Genomics and biochemistry investigation on the metabolic pathway of milled wood and alkali lignin-derived aromatic metabolites of Comamonas serinivorans SP-35

Daochen Zhu, Haibing Si, Peipei Zhang, Alei Geng, more

Biotechnology for Biofuels > 2018 > 11 > 1 > 1-15

Background The efficient depolymerization and utilization of lignin are one of the most important goals for the renewable use of lignocelluloses. The degradation and complete mineralization of lignin by bacteria represent a key step for carbon recycling in land ecosystems as well. However, many aspects of this process remain unclear, for example, the complex network of metabolic pathways involved...

article

Mass spectrometry-based proteomics for system-level characterization of biological responses to engineered nanomaterials

Tong Zhang, Matthew J. Gaffrey, Brian D. Thrall, Wei-Jun Qian

Analytical and Bioanalytical Chemistry > 2018 > 410 > 24 > 6067-6077

The widespread use of engineered nanomaterials or nanotechnology makes the characterization of biological responses to nanomaterials an important area of research. The application of omics approaches, such as mass spectrometry-based proteomics, has revealed new insights into the cellular responses of exposure to nanomaterials, including how nanomaterials interact and alter cellular pathways. In addition,...

article

Advances in microscale separations towards nanoproteomics applications

Lian Yi, Paul D. Piehowski, Tujin Shi, Richard D. Smith, more

Journal of Chromatography A > 2017 > 1523 > C > 40-48

Microscale separation (e.g., liquid chromatography or capillary electrophoresis) coupled with mass spectrometry (MS) has become the primary tool for advanced proteomics, an indispensable technology for gaining understanding of complex biological processes. In recent decades significant advances have been achieved in MS-based proteomics. However, the current proteomics platforms still face an analytical...

article

Fatiguing Contractions Induce Acute Redox Signaling in Mouse Muscle

Philip Kramer, Jicheng Duan, Matthew J. Gaffrey, Lu Wang, more

Free Radical Biology and Medicine > 2017 > 112 > S1 > 191-192

Protein S-glutathionylation (P-SSG) is a reversible redox modification known to alter the activity of transcription factors, kinases, and contractile proteins as well as protect critical cysteine residues from irreversible oxidation. Exercise elicits an acute oxidative stress that is reported to result in beneficial adaptive responses, a process known as redox signaling. We hypothesized that redox...

article

Quantitative proteomics identifies altered O‐GlcNAcylation of structural, synaptic and memory‐associated proteins in Alzheimer's disease

Sheng Wang, Feng Yang, Vladislav A Petyuk, Anil K Shukla, more

The Journal of Pathology > 243 > 1 > 78 - 88

Protein modification by O‐linked β‐N‐acetylglucosamine (O‐GlcNAc) is emerging as an important factor in the pathogenesis of sporadic Alzheimer's disease (AD); however, detailed molecular characterization of this important protein post‐translational modification at the proteome level has been highly challenging, owing to its low stoichiometry and labile nature. Herein, we report the most comprehensive,...

article

Quantification of mutant SPOP proteins in prostate cancer using mass spectrometry-based targeted proteomics

Hui Wang, Christopher E. Barbieri, Jintang He, Yuqian Gao, more

Journal of Translational Medicine > 2017 > 15 > 1 > 1-10

Background Speckle-type POZ protein (SPOP) is an E3 ubiquitin ligase adaptor protein that functions as a potential tumor suppressor, and SPOP mutations have been identified in ~10% of human prostate cancers. However, it remains unclear if mutant SPOP proteins can be utilized as biomarkers for early detection, diagnosis, prognosis or targeted therapy of prostate cancer. Moreover, the SPOP mutation...

article

Biodegradation of alkaline lignin by Bacillus ligniniphilus L1

Daochen Zhu, Peipei Zhang, Changxiao Xie, Weimin Zhang, more

Biotechnology for Biofuels > 2017 > 10 > 1 > 1-14

Background Lignin is the most abundant aromatic biopolymer in the biosphere and it comprises up to 30% of plant biomass. Although lignin is the most recalcitrant component of the plant cell wall, still there are microorganisms able to decompose it or degrade it. Fungi are recognized as the most widely used microbes for lignin degradation. However, bacteria have also been known to be able to utilize...

article

Improved Redox State Increases Aged Skeletal Muscle Performance

Matthew D. Campbell, Gary Knowles, Kevin Kilroy, Ashton T. Samuleson, more

Free Radical Biology and Medicine > 2016 > 100 > S > S81

Muscle redox status regulates diverse aspects of skeletal muscle function, including mitochondrial energetics and contractile performance. In aging skeletal muscle mitochondrial dysfunction contributes to altered redox homeostasis creating a feedforward cycle. Despite this, the role of redox status and its dynamic nature has not been adequately explored as it relates to the decline in skeletal muscle...

article

Contributions of immunoaffinity chromatography to deep proteome profiling of human biofluids

Chaochao Wu, Jicheng Duan, Tao Liu, Richard D. Smith, more

Journal of Chromatography B > 2016 > 1021 > C > 57-68

Human biofluids, especially blood plasma or serum, hold great potential as the sources of candidate biomarkers for various diseases; however, the enormous dynamic range of protein concentrations in biofluids represents a significant analytical challenge for detecting promising low-abundance proteins. Over the last decade, various immunoaffinity chromatographic methods have been developed and routinely...

article

S&T-10 PROSTATE CANCER DIAGNOSIS BY MULTIPLE SECRETED PROTEIN BIOMARKERS IN VOIDED URINE

Tujin Shi, Yuqian Gao, Wei-jun Qian, Robin Leach, more

The Journal of Urology > 2016 > 195 > 4(S) > e312

article

SerpinB1 Promotes Pancreatic β Cell Proliferation

Abdelfattah El Ouaamari, Ercument Dirice, Nicholas Gedeon, Jiang Hu, more

Cell Metabolism > 2016 > 23 > 1 > 194-205

Although compensatory islet hyperplasia in response to insulin resistance is a recognized feature in diabetes, the factor(s) that promote β cell proliferation have been elusive. We previously reported that the liver is a source for such factors in the liver insulin receptor knockout (LIRKO) mouse, an insulin resistance model that manifests islet hyperplasia. Using proteomics we show that serpinB1,...

article

Reducing Oxidative Stress Restores Thiol Proteome and Improves Energetics and Performance in Aged Mouse Skeletal Muscle

Matthew D. Campbell, Gary M. Knowles, Matthew J. Gaffrey, Richard P. Beyer, more

Free Radical Biology and Medicine > 2015 > 87 > S1 > S127

article

Preserved DNA Damage Checkpoint Pathway Protects against Complications in Long-Standing Type 1 Diabetes

Shweta Bhatt, Manoj K. Gupta, Mogher Khamaisi, Rachael Martinez, more

Cell Metabolism > 2015 > 22 > 2 > 239-252

The mechanisms underlying the development of complications in type 1 diabetes (T1D) are poorly understood. Disease modeling of induced pluripotent stem cells (iPSCs) from patients with longstanding T1D (disease duration ≥ 50 years) with severe (Medalist +C) or absent to mild complications (Medalist −C) revealed impaired growth, reprogramming, and differentiation in Medalist +C. Genomics and proteomics...

article

Analytical platform evaluation for quantification of ERG in prostate cancer using protein and mRNA detection methods

Jintang He, Athena A Schepmoes, Tujin Shi, Chaochao Wu, more

Journal of Translational Medicine > 2015 > 13 > 1 > 1-14

Background The established methods for detecting prostate cancer (CaP) are based on tests using PSA (blood), PCA3 (urine), and AMACR (tissue) as biomarkers in patient samples. The demonstration of ERG oncoprotein overexpression due to gene fusion in CaP has thus provided ERG as an additional biomarker. Based on this, we hypothesized that ERG protein quantification methods can be of use in the...

article

Brain Insulin Lowers Circulating BCAA Levels by Inducing Hepatic BCAA Catabolism

Andrew C. Shin, Martin Fasshauer, Nika Filatova, Linus A. Grundell, more

Cell Metabolism > 2014 > 20 > 5 > 898-909

Circulating branched-chain amino acid (BCAA) levels are elevated in obesity/diabetes and are a sensitive predictor for type 2 diabetes. Here we show in rats that insulin dose-dependently lowers plasma BCAA levels through induction of hepatic protein expression and activity of branched-chain α-keto acid dehydrogenase (BCKDH), the rate-limiting enzyme in the BCAA degradation pathway. Selective induction...

article

Targeting Mitochondrial Oxidative Stress to Improve Aged Skeletal Muscle Function

Matthew David Campbell, Ying Ann Chiao, Matthew J Gaffrey, Danijel Djukovic, more

Free Radical Biology and Medicine > 2014 > 76 > Supplement 1 > S29

article

Antibody-independent targeted quantification of TMPRSS2-ERG fusion protein products in prostate cancer

Jintang He, Xuefei Sun, Tujin Shi, Athena A. Schepmoes, more

Molecular Oncology > 2014 > 8 > 7 > 1169-1180

Fusions between the transmembrane protease serine 2 (TMPRSS2) and ETS related gene (ERG) represent one of the most specific biomarkers that define a distinct molecular subtype of prostate cancer. Studies of TMPRSS2-ERG gene fusions have seldom been performed at the protein level, primarily due to the lack of high-quality antibodies suitable for quantitative studies. Herein, we applied a recently developed...

article

Proteomic identification and quantification of S-glutathionylation in mouse macrophages using resin-assisted enrichment and isobaric labeling

Dian Su, Matthew J. Gaffrey, Jia Guo, Kayla E. Hatchell, more

Free Radical Biology and Medicine > 2014 > 67 > Complete > 460-470

S-Glutathionylation (SSG) is an important regulatory posttranslational modification on protein cysteine (Cys) thiols, yet the role of specific cysteine residues as targets of modification is poorly understood. We report a novel quantitative mass spectrometry (MS)-based proteomic method for site-specific identification and quantification of S-glutathionylation across different conditions. Briefly,...

article

Impact of alg3 gene deletion on growth, development, pigment production, protein secretion, and functions of recombinant Trichoderma reesei cellobiohydrolases in Aspergillus niger

Ziyu Dai, Uma K. Aryal, Anil Shukla, Wei-Jun Qian, more

Fungal Genetics and Biology > 2013 > 61 > Complete > 120-132

Dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl α-1,3-mannosyltransferase (also known as “asparagine-linked glycosylation 3”, or ALG3) is involved in early N-linked glycan synthesis and thus is essential for formation of N-linked protein glycosylation. In this study, we examined the effects of alg3 gene deletion (alg3Δ) on growth, development, pigment production, protein secretion and recombinant Trichoderma...

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Search results for: Wei-Jun Qian

Discovery of potential pathways for biological conversion of poplar wood into lipids by co-fermentation of Rhodococci strains

Genomics and biochemistry investigation on the metabolic pathway of milled wood and alkali lignin-derived aromatic metabolites of Comamonas serinivorans SP-35

Mass spectrometry-based proteomics for system-level characterization of biological responses to engineered nanomaterials

Advances in microscale separations towards nanoproteomics applications

Fatiguing Contractions Induce Acute Redox Signaling in Mouse Muscle

Quantitative proteomics identifies altered O‐GlcNAcylation of structural, synaptic and memory‐associated proteins in Alzheimer's disease

Quantification of mutant SPOP proteins in prostate cancer using mass spectrometry-based targeted proteomics

Biodegradation of alkaline lignin by Bacillus ligniniphilus L1

Improved Redox State Increases Aged Skeletal Muscle Performance

Contributions of immunoaffinity chromatography to deep proteome profiling of human biofluids

S&T-10 PROSTATE CANCER DIAGNOSIS BY MULTIPLE SECRETED PROTEIN BIOMARKERS IN VOIDED URINE

SerpinB1 Promotes Pancreatic β Cell Proliferation

Reducing Oxidative Stress Restores Thiol Proteome and Improves Energetics and Performance in Aged Mouse Skeletal Muscle

Preserved DNA Damage Checkpoint Pathway Protects against Complications in Long-Standing Type 1 Diabetes

Analytical platform evaluation for quantification of ERG in prostate cancer using protein and mRNA detection methods

Brain Insulin Lowers Circulating BCAA Levels by Inducing Hepatic BCAA Catabolism

Targeting Mitochondrial Oxidative Stress to Improve Aged Skeletal Muscle Function

Antibody-independent targeted quantification of TMPRSS2-ERG fusion protein products in prostate cancer

Proteomic identification and quantification of S-glutathionylation in mouse macrophages using resin-assisted enrichment and isobaric labeling

Impact of alg3 gene deletion on growth, development, pigment production, protein secretion, and functions of recombinant Trichoderma reesei cellobiohydrolases in Aspergillus niger

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