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This paper reports improvements of atomic force microscopy (AFM) mass spectrometry (MS), in which ∼1 attoliter of analyte is desorbed by a heated AFM cantilever tip and analyzed with a mass spectrometer. Decoupling the AFM sampling apparatus from the MS system enabled analysis of the microscale transport physics independent of analyte ionization efficiency. Using this approach, we find that the transport...
Neuropeptides support inter-cell communication and have a role in many diverse biological processes. For pig, a biomedical model, few prohormones from which neuropeptides result after convertase processing are listed in the UniProt database. Therefore, our goals are to obtain an in silico library of pig prohormone and convertase genes and to functionally annotate these genes based on a large number...
This chapter contains sections titled:
Introduction
Considerations for Single Cell Chemical Microanalysis using Mass Spectrometry
Mass Spectrometry as a Discovery Tool for Chemical Analysis of Cells
Single Cell Mass Spectrometric Applications
Subcellular Profiling
Imaging Single Cells with MS
Signaling Molecule Release from Single Cells
Future Developments
Acknowledgements
References
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