Michael W. Senko

article

STORI Plots Enable Accurate Tracking of Individual Ion Signals

Jared O. Kafader, Steven C. Beu, Bryan P. Early, Rafael D. Melani, more

Journal of The American Society for Mass Spectrometry > 2019 > 30 > 11 > 2200-2203

Charge detection mass spectrometry (CDMS) of low-level signals is currently limited to the analysis of individual ions that generate a persistent signal during the entire observation period. Ions that disintegrate during the observation period produce reduced frequency domain signal amplitudes, which lead to an underestimation of the ion charge state, and thus the ion mass. The charge assignment can...

article

Enhanced Dissociation of Intact Proteins with High Capacity Electron Transfer Dissociation

Nicholas M. Riley, Christopher Mullen, Chad R. Weisbrod, Seema Sharma, more

Journal of The American Society for Mass Spectrometry > 2016 > 27 > 3 > 520-531

Electron transfer dissociation (ETD) is a valuable tool for protein sequence analysis, especially for the fragmentation of intact proteins. However, low product ion signal-to-noise often requires some degree of signal averaging to achieve high quality MS/MS spectra of intact proteins. Here we describe a new implementation of ETD on the newest generation of quadrupole-Orbitrap-linear ion trap Tribrid,...

article

Performance evaluation of a dual linear ion trap-Fourier transform ion cyclotron resonance mass spectrometer for proteomics research

Chad R. Weisbrod, Michael R. Hoopmann, Michael W. Senko, James E. Bruce

Journal of Proteomics > 2013 > 88 > Complete > 109-119

A novel dual cell linear ion trap Fourier transform ion cyclotron resonance mass spectrometer (FT-ICR MS) and its performance characteristics are reported. A linear ion trap-Fourier transform ion cyclotron resonance mass spectrometer has been modified to incorporate a LTQ-Velos mass spectrometer. This modified instrument features efficient ion accumulation and fast MS/MS acquisition capabilities of...

article

High-Performance Mass Spectrometry: Fourier Transform Ion Cyclotron Resonance at 14.5 Tesla

Tanner M. Schaub, Christopher L. Hendrickson, Stevan Horning, John P. Quinn, more

Analytical Chemistry > 2008 > 80 > 11 > 3985 - 3990

article

New and Automated MSn Approaches for Top-Down Identification of Modified Proteins

Vlad Zabrouskov, Michael W. Senko, Yi Du, Richard D. Leduc, more

Journal of the American Society for Mass Spectrometry > 2005 > 16 > 12 > 2027-2038

An automated top-down approach including data-dependent MS ³ experiment for protein identification/characterization is described. A mixture of wild-type yeast proteins has been separated on-line using reverse-phase liquid chromatography and introduced into a hybrid linear ion trap (LTQ) Fourier transform ion cylclotron resonance (FTICR) mass spectrometer, where the most abundant molecular...

article

New and automated MSⁿ approaches for top-down identification of modified proteins

Vlad Zabrouskov, Michael W. Senko, Yi Du, Richard D. Leduc, more

Journal of The American Society for Mass Spectrometry > 2005 > 16 > 12 > 2027-2038

An automated top-down approach including data-dependent MS³ experiment for protein identification/characterization is described. A mixture of wild-type yeast proteins has been separated on-line using reverse-phase liquid chromatography and introduced into a hybrid linear ion trap (LTQ) Fourier transform ion cylclotron resonance (FTICR) mass spectrometer, where the most abundant molecular ions were...

article

Novel Linear Quadrupole Ion Trap/FT Mass Spectrometer: Performance Characterization and Use in the Comparative Analysis of Histone H3 Post-translational Modifications

John E. P. Syka, Jarrod A. Marto, Dina L. Bai, Stevan Horning, more

Journal of Proteome Research > 2004 > 3 > 3 > 621 - 626

article

A two-dimensional quadrupole ion trap mass spectrometer

Jae C. Schwartz, Michael W. Senko, John E.P. Syka

Journal of the American Society for Mass Spectrometry > 2002 > 13 > 6 > 659-669

The use of a linear or two-dimensional (2-D) quadrupole ion trap as a high performance mass spectrometer is demonstrated. Mass analysis is performed by ejecting ions out a slot in one of the rods using the mass selective instability mode of operation. Resonance ejection and excitation are utilized to enhance mass analysis and to allow isolation and activation of ions for MS ⁿ capability. Improved...

article

A two-dimensional quadrupole ion trap mass spectrometer

Jae C. Schwartz, Michael W. Senko, John E. P. Syka

Journal of The American Society for Mass Spectrometry > 2002 > 13 > 6 > 659-669

The use of a linear or two-dimensional (2-D) quadrupole ion trap as a high performance mass spectrometer is demonstrated. Mass analysis is performed by ejecting ions out a slot in one of the rods using the mass selective instability mode of operation. Resonance ejection and excitation are utilized to enhance mass analysis and to allow isolation and activation of ions for MSⁿ capability. Improved trapping...

article

External Accumulation of Ions for Enhanced Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry - Analytical Applications of Fourier Transform Ion Cyclotron Resonance Mass Spectrometry

Michael W. Senko, Christopher L. Hendrickson, Mark R. Emmett, Stone D.-H. Shi, more

Journal of the American Society for Mass Spectrometry > 1997 > 8 > 9 > 970-976

Electrospray ionization (ESI) in combination with Fourier transform ion cyclotron resonance (FTICR) mass spectrometry provides for mass analysis of biological molecules with unrivaled mass accuracy, resolving power and sensitivity. However, ESI FTICR MS performance with on-line separation techniques such as liquid chromatography (LC) and capillary electrophoresis has to date been limited primarily...

article

Unit Resolution Mass Spectra of 112 kDa Molecules with 3 Da Accuracy

Neil L. Kelleher, Michael W. Senko, Marshall M. Siegel, Fred W. McLafferty

Journal of the American Society for Mass Spectrometry > 1997 > 8 > 4 > 380-383

Previously, the highest molecular weight for a sample yielding a unit resolution mass spectrum was 67 kDa (marginal at 86 kDa), obtained with a 6.2 T Fourier-transform mass spectrometer with electrospray ionization. Now with a 9.4 T instrument, resolving power of 170,000 has been achieved for chondroitinase I (997 amino acids) and II (990 amino acids), making possible molecular weight assignments...

article

External accumulation of ions for enhanced electrospray ionization fourier transform ion cyclotron resonance mass spectrometry

Michael W. Senko, Christopher L. Hendrickson, Mark R. Emmett, Stone D. H. Shi, more

Journal of The American Society for Mass Spectrometry > 1997 > 8 > 9 > 970-976

Electrospray ionization (ESI) in combination with Fourier transform ion cyclotron resonance (FTICR) mass spectrometry provides for mass analysis of biological molecules with unrivaled mass accuracy, resolving power and sensitivity. However, ESI FTICR MS performance with on-line separation techniques such as liquid chromatography (LC) and capillary electrophoresis has to date been limited primarily...

article

Unit resolution mass spectra of 112 kDa molecules with 3 Da accuracy

Neil L. Kelleher, Michael W. Senko, Marshall M. Siegel, Fred W. McLafferty

Journal of The American Society for Mass Spectrometry > 1997 > 8 > 4 > 380-383

Previously, the highest molecular weight for a sample yielding a unit resolution mass spectrum was 67 kDa (marginal at 86 kDa), obtained with a 6. 2 T Fourier-transform mass spectrometer with electrospray ionization. Now with a 9. 4 T instrument, resolving power of 170,000 has been achieved for chondroitinase I (997 amino acids) and II (990 amino acids), making possible molecular weight assignments...

article

Determination of monoisotopic masses and ion populations for large biomolecules from resolved isotopic distributions

Michael W. Senko, Steven C. Beu, Fred W. McLafferty

Journal of the American Society for Mass Spectrometry > 1995 > 6 > 4 > 229-233

The coupling of electrospray ionization with Fourier-transform mass spectrometry allows the analysis of large biomolecules with mass-measuring errors of less than 1 ppm. The large number of atoms incorporated in these molecules results in a low probability for the all-monoisotopic species. This produces the potential to misassign the number of heavy isotopes in a specific peak and make a mass error...

article

Complete large-molecule high-resolution mass spectra from 50-femtomole microvolume injection

Neil L. Kelleher, Michael W. Senko, Daniel P. Little, Peter B. O'Connor, more

Journal of the American Society for Mass Spectrometry > 1995 > 6 > 3 > 220-221

For electrospray ionization in Fourier-transform mass spectrometry, direct injection of 5 × 10 ⁻¹⁴ mol (0.5 μL of 100 nM from a microvolume sample valve) of ubiquitin (8565 Da) into the flowing solvent stream yields a spectrum with 85:1 signal-to-noise ratio, 2-ppm mass accuracy, and isotopic resolution. Gated trapping for 100 μs from a 0.15-μL/min injection of 20-μM ubiquitin consumes 5 ×...

article

Automated assignment of charge states from resolved isotopic peaks for multiply charged ions

Michael W. Senko, Steven C. Beu, Fred W. McLafferty

Journal of the American Society for Mass Spectrometry > 1995 > 6 > 1 > 52-56

The recent proliferation of electrospray as an ionization method has greatly increased the ability to perform analyses of large biomolecules by using mass spectrometry. The major advantage of electrospray is the ability to produce multiply charged ions, which brings large molecules down to a mass-to-charge ratio range amenable to most instruments. Multiple charging is also a disadvantage because mass...

INFONA - science communication portal

Search results for: Michael W. Senko

STORI Plots Enable Accurate Tracking of Individual Ion Signals

Enhanced Dissociation of Intact Proteins with High Capacity Electron Transfer Dissociation

Performance evaluation of a dual linear ion trap-Fourier transform ion cyclotron resonance mass spectrometer for proteomics research

High-Performance Mass Spectrometry: Fourier Transform Ion Cyclotron Resonance at 14.5 Tesla

New and Automated MSn Approaches for Top-Down Identification of Modified Proteins

New and automated MSⁿ approaches for top-down identification of modified proteins

Novel Linear Quadrupole Ion Trap/FT Mass Spectrometer: Performance Characterization and Use in the Comparative Analysis of Histone H3 Post-translational Modifications

A two-dimensional quadrupole ion trap mass spectrometer

A two-dimensional quadrupole ion trap mass spectrometer

External Accumulation of Ions for Enhanced Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry - Analytical Applications of Fourier Transform Ion Cyclotron Resonance Mass Spectrometry

Unit Resolution Mass Spectra of 112 kDa Molecules with 3 Da Accuracy

External accumulation of ions for enhanced electrospray ionization fourier transform ion cyclotron resonance mass spectrometry

Unit resolution mass spectra of 112 kDa molecules with 3 Da accuracy

Determination of monoisotopic masses and ion populations for large biomolecules from resolved isotopic distributions

Complete large-molecule high-resolution mass spectra from 50-femtomole microvolume injection

Automated assignment of charge states from resolved isotopic peaks for multiply charged ions

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