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When Euglena gracilis was grown in the heterotrophic condition with glucose and (NH4)2SO4 as the carbon and nitrogen source, a high cell yield (4.28–4.48 g l−1) was obtained and the culture pH decreased to 1.6–2. The biomass production in the heterotrophic culture was compared to those in the autotrophic and mixotrophic cultures. Autotrophic growth was 4.7–6.3% of the heterotrophic one, whereas about...
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