Search results for: Frank P. Barry

Items from 1 to 20 out of 20 results

article

Thermoresponsive Substrates Used for the Growth and Controlled Differentiation of Human Mesenchymal Stem Cells

Xingliang Fan, Maria E. Nash, Alexander V. Gorelov, Frank P. Barry, more

Macromolecular Rapid Communications > 36 > 21 > 1897 - 1901

This communication outlines the advances made in the development of thermoresponsive substrates for human mesenchymal stem cell (hMSC) expansion and subsequent controlled specific and multilineage differentiation from a previous study performed by this group. Previously, the development of an inexpensive and technically accessible method for hMSC expansion and harvesting was reported, using the solvent...

article

ROCK activity and the Gβγ complex mediate chemotactic migration of mouse bone marrow-derived stromal cells

Caroline M. Ryan, James A. L. Brown, Emer Bourke, Áine M. Prendergast, more

Stem Cell Research & Therapy > 2015 > 6 > 1 > 1-15

Introduction Bone marrow-derived stromal cells (BMSCs), also known as mesenchymal stem cells, are the focus of intensive efforts worldwide to elucidate their function and biology. Despite the importance of BMSC migration for their potential therapeutic uses, the mechanisms and signalling governing stem cell migration are still not fully elucidated. Methods We investigated and detailed the effects...

article

A chondromimetic microsphere for in situ spatially controlled chondrogenic differentiation of human mesenchymal stem cells

Sharon Ansboro, Jessica S. Hayes, Valerie Barron, Shane Browne, more

Journal of Controlled Release > 2014 > 179 > Complete > 42-51

Human mesenchymal stem cells (hMSCs) have been identified as a viable cell source for cartilage tissue engineering. However, to undergo chondrogenic differentiation hMSCs require growth factors, in particular members of the transforming growth factor beta (TGF-β) family. While in vitro differentiation is feasible through continuous supplementation of TGF-β3, mechanisms to control and drive hMSCs down...

article

Impact of Mesenchymal Stem Cell secreted PAI-1 on colon cancer cell migration and proliferation

Niamh M. Hogan, Myles R. Joyce, J. Mary Murphy, Frank P. Barry, more

Biochemical and Biophysical Research Communications > 2013 > 435 > 4 > 574-579

Mesenchymal Stem Cells are known to engraft and integrate into the architecture of colorectal tumours, with little known regarding their fate following engraftment. This study aimed to investigate mediators of Mesenchymal Stem Cell (MSC) and colon cancer cell (CCC) interactions. Mesenchymal Stem Cells and colon cancer cells (HT29 and HCT-116) were cultured individually or in co-culture on 3-dimensional...

article

High levels of ephrinB2 over-expression increases the osteogenic differentiation of human mesenchymal stem cells and promotes enhanced cell mediated mineralisation in a polyethyleneimine-ephrinB2 gene-activated matrix

Erica G. Tierney, Kevin McSorley, Conn L. Hastings, Sally-Ann Cryan, more

Journal of Controlled Release > 2013 > 165 > 3 > 173-182

Gene therapy can be combined with tissue engineering constructs to produce gene-activated matrices (GAMs) with enhanced capacity for repair. Polyethyleneimine (PEI), a non-viral vector, has previously been optimised for high efficiency gene transfer in rat mesenchymal stem cells (rMSCs). The use of PEI to transfect human MSCs (hMSCs) with ephrinB2 is assessed here. Recently a role for the ephrinB2...

article

Thermoresponsive Substrates used for the Expansion of Human Mesenchymal Stem Cells and the Preservation of Immunophenotype

Maria E. Nash, Xingliang Fan, William M. Carroll, Alexander V. Gorelov, more

Stem Cell Reviews and Reports > 2013 > 9 > 2 > 148-157

The facile regeneration of undifferentiated human mesenchymal stem cells (hMSCs) from thermoresponsive surfaces facilitates the collection of stem cells avoiding the use of animal derived cell detachment agents commonly used in cell culture. This communication proposes a procedure to fabricate coatings from commercially available pNIPAm which is both affordable and a significant simplification on...

article

The electrical stimulation of carbon nanotubes to provide a cardiomimetic cue to MSCs

Emma Mooney, Joseph N. Mackle, David J.-P. Blond, Eoin O'Cearbhaill, more

Biomaterials > 2012 > 33 > 26 > 6132-6139

Once damaged, cardiac muscle has little intrinsic repair capability due to the poor regeneration potential of remaining cardiomyocytes. One method of overcoming this issue is to deliver functional cells to the injured myocardium to promote repair. To address this limitation we sought to test the hypothesis that electroactive carbon nanotubes (CNT) could be employed to direct mesenchymal stem cell...

article

Development of a thermoresponsive chitosan gel combined with human mesenchymal stem cells and desferrioxamine as a multimodal pro-angiogenic therapeutic for the treatment of critical limb ischaemia

Conn L. Hastings, Helena M. Kelly, Mary J. Murphy, Frank P. Barry, more

Journal of Controlled Release > 2012 > 161 > 1 > 73-80

Critical limb ischaemia (CLI) is a debilitating ischaemic disease caused by vascular occlusion. Pro-angiogenic therapeutics have the potential to produce collateral vasculature, delaying or negating the need for amputation or invasive revascularisation. Thermoresponsive hydrogels can provide an in situ depot for the sustained release of drugs and provide protection and cohesion for encapsulated cells...

article

Doxorubicin induces the DNA damage response in cultured human mesenchymal stem cells

Séverine Cruet-Hennequart, Áine M. Prendergast, Georgina Shaw, Frank P. Barry, more

International Journal of Hematology > 2012 > 96 > 5 > 649-656

Anthracyclines, including doxorubicin, are widely used in the treatment of leukemia. While the effects of doxorubicin on hematopoietic cells have been characterized, less is known about the response of human mesenchymal stem cells (hMSCs) in the bone marrow stroma to anthracyclines. We characterized the effect of doxorubicin on key DNA damage responses in hMSCs, and compared doxorubicin sensitivity...

article

Mesenchymal stem cell inhibition of T‐helper 17 cell‐ differentiation is triggered by cell–cell contact and mediated by prostaglandin E2 via the EP4 receptor

Michelle M. Duffy, Jana Pindjakova, Shirley A. Hanley, Cathal McCarthy, more

European Journal of Immunology > 41 > 10 > 2840 - 2851

Mesenchymal stem cells (MSCs) inhibit T‐cell activation and proliferation but their effects on individual T‐cell‐effector pathways and on memory versus naïve T cells remain unclear. MSC influence on the differentiation of naïve and memory CD4⁺ T cells toward the Th17 phenotype was examined. CD4⁺ T cells exposed to Th17‐skewing conditions exhibited reduced CD25 and IL‐17A expression following MSC co‐culture...

article

In vitro Characterization of an Electroactive Carbon‐Nanotube‐Based Nanofiber Scaffold for Tissue Engineering

Joseph N. Mackle, David J.‐P. Blond, Emma Mooney, Caitlin McDonnell, more

Macromolecular Bioscience > 11 > 9 > 1272 - 1282

In an effort to reduce organ replacement and enhance tissue repair, there has been a tremendous effort to create biomechanically optimized scaffolds for tissue engineering applications. In contrast, the development and characterization of electroactive scaffolds has attracted little attention. Consequently, the creation and characterization of a carbon nanotube based poly(lactic acid) nanofiber scaffold...

article

Mesenchymal chondroprogenitor cell origin and therapeutic potential

Janice O'Sullivan, Sinéad D'Arcy, Frank P Barry, JMary Murphy, more

Stem Cell Research & Therapy > 2011 > 2 > 1 > 1-7

Mesenchymal progenitor cells, a multipotent adult stem cell population, have the ability to differentiate into cells of connective tissue lineages, including fat, cartilage, bone and muscle, and therefore generate a great deal of interest for their potential use in regenerative medicine. During development, endochondral bone is formed from a template of cartilage that transforms into bone; however,...

article

Potential of rat bone marrow-derived mesenchymal stem cells as vehicles for delivery of neurotrophins to the Parkinsonian rat brain

Teresa C. Moloney, Gemma E. Rooney, Frank P. Barry, Linda Howard, more

Brain Research > 2010 > 1359 > Complete > 33-43

Issues related to the intra-cerebral delivery of glial cell line-derived neurotrophic factor (GDNF) have hampered its progression as a neuroprotective therapy for Parkinson's disease. Ex vivo gene therapy, where cells are virally transduced in vitro to produce a specific protein, may circumvent some of the problems associated with direct delivery of this neurotrophin to the brain. In this regard,...

article

Advances in mesenchymal stem cell-mediated gene therapy for cancer

Roisin M Dwyer, Sonja Khan, Frank P Barry, Timothy O'Brien, more

Stem Cell Research & Therapy > 2010 > 1 > 3 > 1-7

Mesenchymal stem cells have a natural tropism for tumours and their metastases, and are also considered immunoprivileged. This remarkable combination of properties has formed the basis for many studies investigating their potential as tumour-specific delivery vehicles for suicide genes, oncolytic viruses and secreted therapeutic proteins. The aim of the present review is to discuss the range of approaches...

article

Stem Cell Therapy and Regenerative Medicine

Timothy O'Brien, Frank P. Barry

Mayo Clinic Proceedings > 2009 > 84 > 10 > 859-861

article

Response of mesenchymal stem cells to the biomechanical environment of the endothelium on a flexible tubular silicone substrate

Eoin D. O'Cearbhaill, Marie A. Punchard, Mary Murphy, Frank P. Barry, more

Biomaterials > 2008 > 29 > 11 > 1610-1619

Understanding the response of mesenchymal stem cells (MSCs) to forces in the vasculature is very important in the field of cardiovascular intervention for a number of reasons. These include the development of MSC seeded tissue engineered vascular grafts, targeted or systemic delivery of MSCs in the dynamic environment of the coronary artery and understanding the potential pathological calcifying role...

article

Murine mesenchymal stem cells suppress dendritic cell migration, maturation and antigen presentation

Karen English, Frank P. Barry, Bernard P. Mahon

Immunology Letters > 2008 > 115 > 1 > 50-58

Mesenchymal stem cells (MSC) possess a wide range of immunosuppressive functions. Among these is the ability to inhibit CD4 ⁺ T cell proliferation. Dendritic cells (DC) play a role in initiating cell-mediated immunity; however, the immunosuppressive influence of MSC on professional antigen presenting cells remains unclear. DC exposed to TNF-α and cultured with murine MSC failed to show regular...

article

IFN-γ and TNF-α differentially regulate immunomodulation by murine mesenchymal stem cells

Karen English, Frank P. Barry, Ciara P. Field-Corbett, Bernard P. Mahon

Immunology Letters > 2007 > 110 > 2 > 91-100

Murine mesenchymal stem cells (MSC) have the ability to inhibit allogeneic immune responses. Two different mechanisms, either cell contact-dependent or independent, have been proposed to account for this immunosuppression. The focus of this study was to elucidate the involvement of soluble suppressive factors secreted by murine MSC in an inflammatory setting, and their role in MSC immunomodulation...

article

Mesenchymal stem cells: clinical applications and biological characterization

Frank P. Barry, J. Mary Murphy

International Journal of Biochemistry and Cell Biology > 2004 > 36 > 4 > 568-584

Mesenchymal stem cells (MSCs) have been isolated from bone marrow, periosteum, trabecular bone, adipose tissue, synovium, skeletal muscle and deciduous teeth. These cells have the capacity to differentiate into cells of connective tissue lineages, including bone, fat, cartilage and muscle. A great deal has been learned in recent years about the isolation and characterization of MSCs, and control of...

article

The Monoclonal Antibody SH-2, Raised against Human Mesenchymal Stem Cells, Recognizes an Epitope on Endoglin (CD105)

Frank P. Barry, Raymond E. Boynton, Stephen Haynesworth, J.Mary Murphy, more

Biochemical and Biophysical Research Communications > 1999 > 265 > 1 > 134-139

Mesenchymal stem cells are multipotent cells resident in the bone marrow throughout adulthood which have the capacity to differentiate into cartilage, bone, fat, muscle, and tendon. A number of monoclonal antibodies raised against human MSCs have been shown to react with surface antigens on these cells in vitro. A protein of molecular mass 92 kDa was immunoprecipitated using the SH-2 monoclonal antibody...

Filter options

Publication date

Set your own date range

INFONA - science communication portal

Search results for: Frank P. Barry

Add recipient

Sending message cancelled

Are you sure you want to cancel sending this message?

Send message

Filter options

Publication date

Date range setting

Set the date range to filter the displayed results. You can set a starting date, ending date or both. You can enter the dates manually or choose them from the calendar.

Content availability

Keywords

Data set

Journal

Reporting an error / abuse

Sending the report failed

Accessibility options