Search results for: Robert T. Kennedy

Items from 1 to 20 out of 116 results

article

Mass Activated Droplet Sorting (MADS) Enables High‐Throughput Screening of Enzymatic Reactions at Nanoliter Scale

Daniel A. Holland‐Moritz, Michael K. Wismer, Benjamin F. Mann, Iman Farasat, more

Angewandte Chemie International Edition > 59 > 11 > 4470 - 4477

Microfluidic droplet sorting enables the high‐throughput screening and selection of water‐in‐oil microreactors at speeds and volumes unparalleled by traditional well‐plate approaches. Most such systems sort using fluorescent reporters on modified substrates or reactions that are rarely industrially relevant. We describe a microfluidic system for high‐throughput sorting of nanoliter droplets based...

article

Mass Activated Droplet Sorting (MADS) Enables High‐Throughput Screening of Enzymatic Reactions at Nanoliter Scale

Daniel A. Holland‐Moritz, Michael K. Wismer, Benjamin F. Mann, Iman Farasat, more

Angewandte Chemie > 132 > 11 > 4500 - 4507

article

CE‐MS with electrokinetic supercharging and application to determination of neurotransmitters

Shane S. Wells, Mohamed Dawod, Robert T. Kennedy

ELECTROPHORESIS > 40 > 22 > 2946 - 2953

Electrokinetic supercharging (EKS) is known as one of the most effective online electrophoretic preconcentration techniques, though pairing with it with mass spectrometry has presented challenges. Here, EKS is successfully paired with ESI‐MS/MS to provide a sensitive and robust method for analysis of biogenic amines in biological samples. Injection parameters including electric field strength and...

article

Incentive and dopamine sensitization produced by intermittent but not long access cocaine self‐administration

Alex B. Kawa, Alec C. Valenta, Robert T. Kennedy, Terry E. Robinson

European Journal of Neuroscience > 50 > 4 > 2663 - 2682

The temporal pattern of drug use (pharmacokinetics) has a profound effect on the ability of self‐administered cocaine to produce addiction‐like behavior in rodents, and to change the brain. To further address this issue, we compared the effects of long access (LgA) cocaine self‐administration, which is widely used to model the transition to addiction, with intermittent access (IntA), which is thought...

article

Varying the rate of intravenous cocaine infusion influences the temporal dynamics of both drug and dopamine concentrations in the striatum

Ellie‐Anna Minogianis, Waqqas M. Shams, Omar S. Mabrouk, Jenny‐Marie T. Wong, more

European Journal of Neuroscience > 50 > 3 > 2054 - 2064

The faster drugs of abuse reach the brain, the greater is the risk of addiction. Even small differences in the rate of drug delivery can influence outcome. Infusing cocaine intravenously over 5 vs. 90–100 s promotes sensitization to the psychomotor and incentive motivational effects of the drug and preferentially recruits mesocorticolimbic regions. It remains unclear whether these effects are due...

article

Droplet sample introduction to microchip gel and zone electrophoresis for rapid analysis of protein-protein complexes and enzymatic reactions

Claire M. Ouimet, Cara I. D’Amico, Robert T. Kennedy

Analytical and Bioanalytical Chemistry > 2019 > 411 > 23 > 6155-6163

Electrophoresis has demonstrated utility as tool for screening of small molecule modulators of protein-protein interactions and enzyme targets. Screening of large chemical libraries requires high-throughput separations. Such fast separation can be accessed by microchip electrophoresis. Here, microchip gel electrophoresis separations of proteins are achieved in 2.6 s with 1200 V/cm and 3-mm separation...

article

Detection and quantification of vitamins in microliter volumes of biological samples by LC‐MS for clinical screening

Maryam Khaksari, Lynn R. Mazzoleni, Chunhai Ruan, Peng Song, more

AIChE Journal > 64 > 10 > 3709 - 3718

A method for simultaneous determination of water‐soluble vitamins B₁, B₂, B₃ (nicotinamide), B₅, B₆ (pyridoxamine), B₉ and fat‐soluble vitamins E (α‐tocopherol) and K₁ in tears, and B₁, B₂, B₃, B₅, B₆, B₉, A (retinol), and E in blood serum is described. Liquid chromatography‐mass spectrometry (LC‐MS) was used with a ternary mobile phase of water and acetonitrile containing 0.1% formic acid and methanol...

article

Determination of amines and phenolic acids in wine with benzoyl chloride derivatization and liquid chromatography–mass spectrometry

Paige A. Malec, Marianna Oteri, Veronica Inferrera, Francesco Cacciola, more

Journal of Chromatography A > 2017 > 1523 > C > 248-256

Amine and phenolic metabolites are important contributors to the flavor and health effects of many foods, including wine. Determination of these metabolites often involves UV detection following separation by liquid chromatography. While this is sufficient for some applications, chemical derivatization with LC–MS provides greater sensitivity and selectivity relative to LC-UV.We have developed an assay...

article

Pushing the boundaries of chromatography and electrophoresis—Honoring James Jorgenson on his 65th birthday

James P. Grinias, Robert T. Kennedy

Journal of Chromatography A > 2017 > 1523 > C > 1-2

article

Microdialysis Coupled with LC-MS/MS for In Vivo Neurochemical Monitoring

Alexander G. Zestos, Robert T. Kennedy

The AAPS Journal > 2017 > 19 > 5 > 1284-1293

Microdialysis is a powerful sampling technique used to monitor small molecules in vivo. Despite the many applications of microdialysis sampling, it is limited by the method of analyzing the resulting samples. An emerging technique for analysis of microdialysis samples is liquid chromatography-tandem mass spectrometry (LC-MS/MS). This technique is highly versatile, allowing multiplexed analysis of...

article

Circuit dynamics of in vivo dynorphn release in the nucleus accumbens

Ream Al-Hasani, Jenny M. Wong, Jordan G. McCall, Omar S. Mabrouk, more

Alcohol > 2017 > 60 > C > 220

article

Data representing two separate LC-MS methods for detection and quantification of water-soluble and fat-soluble vitamins in tears and blood serum

Maryam Khaksari, Lynn R. Mazzoleni, Chunhai Ruan, Robert T. Kennedy, more

Data in Brief > 2017 > 11 > C > 316-330

Two separate liquid chromatography (LC)-mass spectrometry (MS) methods were developed for determination and quantification of water-soluble and fat-soluble vitamins in human tear and blood serum samples. The water-soluble vitamin method was originally developed to detect vitamins B₁, B₂, B₃ (nicotinamide), B₅, B₆ (pyridoxine), B₇, B₉ and B₁₂ while the fat-soluble vitamin method detected vitamins A,...

article

Age-dependent dopamine transporter dysfunction and Serine129 phospho-α-synuclein overload in G2019S LRRK2 mice

Francesco Longo, Daniela Mercatelli, Salvatore Novello, Ludovico Arcuri, more

Acta Neuropathologica Communications > 2017 > 5 > 1 > 1-17

Mutations in the leucine-rich repeat kinase 2 (LRRK2) gene are the most common genetic cause of Parkinson’s disease. Here, we investigated whether the G2019S LRRK2 mutation causes morphological and/or functional changes at nigro-striatal dopamine neurons. Density of striatal dopaminergic terminals, nigral cell counts, tyrosine hydroxylase protein levels as well as exocytotic dopamine release measured...

article

Determination of water-soluble and fat-soluble vitamins in tears and blood serum of infants and parents by liquid chromatography/mass spectrometry

Maryam Khaksari, Lynn R. Mazzoleni, Chunhai Ruan, Robert T. Kennedy, more

Experimental Eye Research > 2017 > 155 > C > 54-63

Tears serve as a viable diagnostic fluid with advantages including less invasive sample to collect and less complex to prepare for analysis. Several water-soluble and fat-soluble vitamins were detected and quantified in human tears and compared with blood serum levels. Samples from 15 family pairs, each pair consisting of a four-month-old infant and one parent were analyzed; vitamin concentrations...

article

Blunted mGluR Activation Disinhibits Striatopallidal Transmission in Parkinsonian Mice

Qiaoling Cui, Jason E. Pitt, Arin Pamukcu, Jean-Francois Poulin, more

Cell Reports > 2016 > 17 > 9 > 2431-2444

The prevailing circuit model predicts that hyperactivity of the striatopallidal pathway and subsequently increased inhibition of external globus pallidus (GPe) neurons lead to the hypokinetic symptoms of Parkinson’s disease (PD). It is believed that hyperactivity of the striatopallidal pathway is due to inactivity of dopamine receptors on the somatodendritic membrane of striatopallidal neurons, but...

article

Design and microfabrication of a miniature fiber optic probe with integrated lenses and mirrors for Raman and fluorescence measurements

Thitaphat Ngernsutivorakul, Cynthia M. Cipolla, Colleen E. Dugan, Shi Jin, more

Analytical and Bioanalytical Chemistry > 2017 > 409 > 1 > 275-285

Fiber optics coupled to components such as lenses and mirrors have seen extensive use as probes for Raman and fluorescence measurements. Probes can be placed directly on or into a sample to allow for simplified and remote application of these optical techniques. The size and complexity of such probes however limits their application. We have used microfabrication in polydimethylsiloxane (PDMS) to...

article

Monitoring cell secretions on microfluidic chips using solid-phase extraction with mass spectrometry

Colleen E. Dugan, James P. Grinias, Sebastian D. Parlee, Mahmoud El-Azzouny, more

Analytical and Bioanalytical Chemistry > 2017 > 409 > 1 > 169-178

Microfluidics is an enabling technology for both cell biology and chemical analysis. We combine these attributes with a microfluidic device for on-line solid-phase extraction (SPE) and mass spectrometry (MS) analysis of secreted metabolites from living cells in culture on the chip. The device was constructed with polydimethylsiloxane (PDMS) and contains a reversibly sealed chamber for perfusing cells...

article

Knockdown of ATP citrate lyase in pancreatic beta cells does not inhibit insulin secretion or glucose flux and implicates the acetoacetate pathway in insulin secretion

Mahmoud El Azzouny, Melissa J. Longacre, Israr-ul H. Ansari, Robert T. Kennedy, more

Molecular Metabolism > 2016 > 5 > 10 > 980-987

Glucose-stimulated insulin secretion in pancreatic beta cells requires metabolic signals including the generation of glucose-derived short chain acyl-CoAs in the cytosol from mitochondrially-derived metabolites. One concept of insulin secretion is that ATP citrate lyase generates short chain acyl-CoAs in the cytosol from mitochondrially-derived citrate. Of these, malonyl-CoA, is believed to be an...

article

Repeatability of gradient ultrahigh pressure liquid chromatography–tandem mass spectrometry methods in instrument-controlled thermal environments

James P. Grinias, Jenny-Marie T. Wong, Robert T. Kennedy

Journal of Chromatography A > 2016 > 1461 > C > 42-50

The impact of viscous friction on eluent temperature and column efficiency in liquid chromatography is of renewed interest as the need for pressures exceeding 1000bar to use with columns packed with sub-2μm particles has grown. One way the development of axial and radial temperature gradients that arise due to viscous friction can be affected is by the thermal environment the column is placed in....

article

Advances in and prospects of microchip liquid chromatography

James P. Grinias, Robert T. Kennedy

TrAC Trends in Analytical Chemistry > 2016 > 81 > C > 110-117

This study focuses on recent advances (from January 2013 through April 2015) in microfluidic liquid chromatography. Articles published during this period are organized by the type of stationary-phase support focusing on device fabrication, column preparation, and specific applications. In addition, a comprehensive table comparing chromatographic figures of merit for this study is provided in Appendix...

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MICRODIALYSIS (18)
MASS SPECTROMETRY (12)
AMPHETAMINE (5)
CAPILLARY ELECTROPHORESIS (5)
DOPAMINE (5)
ELECTROPHORESIS (5)
GLUTAMATE (5)
MICROFLUIDICS (5)
STRIATUM (5)
CAPILLARY LIQUID CHROMATOGRAPHY (4)
INSULIN (4)
BLOOD SERUM (3)
INTEGRATION (3)
ADDICTION (2)
ADIPOCYTES (2)
AMINO ACIDS (2)
ASCORBATE (2)
AUTOMATION (2)
DROPLETS (2)
ENKEPHALINS (2)
ENZYME ASSAY (2)
FAT-SOLUBLE VITAMIN (2)
GABA (2)
GLUCAGON (2)
IMMUNOASSAY (2)
INFANT (2)
ISLETS OF LANGERHANS (2)
LASER-INDUCED FLUORESCENCE (2)
LIQUID CHROMATOGRAPHY (2)
METABOLOMICS (2)
MICROFLUIDIC (2)
NEUROCHEMISTRY (2)
NEUROPEPTIDE (2)
NEUROTRANSMITTER (2)
NEUROTRANSMITTERS (2)
PARENT (2)
SEGMENTED FLOW (2)
TEAR (2)
TEMPORAL RESOLUTION (2)
TYROSINE HYDROXYLASE (2)
WATER-SOLUBLE VITAMIN (2)
SPATIAL ORGANIZATION; EXOCYTOSIS; CA 2 + ENTRY; INSULIN SECRETION; FLUORESCENCE IMAGING; ZINC; ZINQUIN (1)
6-OHDA (1)
ACETOACETATE PATHWAY (1)
ACETYL-COA (1)
ACETYLCHOLINE (1)
ADENOSINE (1)
ADIPOCYTE (1)
AFFINITY PROBE CAPILLARY ELECTROPHORESIS (1)
AMINES (1)
AMPEROMETRIC DETECTOR (1)
ANAESTHETICS (1)
ANTIBODIES (1)
ANTIGENS (1)
ASCORBIC ACID (1)
ASTROCYTES (1)
BASAL GANGLIA (1)
BEHAVING (1)
BEHAVIORS (1)
BENZOYL CHLORIDE (1)
BIOCATALYSIS (1)
BIOGENIC AMINES (1)
BIOINFORMATICS (1)
BIOKATALYSE (1)
BIOLOGICAL SAMPLES (1)
BIOSENSOR (1)
BRAIN (1)
BRAIN TISSUE (1)
BRAIN TISSUE DAMAGE (1)
CALCIUM (1)
CAPILLARY ELECTROPHORESIS/ELECTROPHORESIS (1)
CAPILLARY ZONE ELECTROPHORESIS (1)
CATECHOLAMINES (1)
CE (1)
CELL VIABILITY (1)
CELLS ON CHIPS (1)
CEREBRAL CORTEX (1)
CE‐MS (1)
CHLORAL HYDRATE (1)
CITRATE (1)
COCAINE (1)
COCAINE ADDICTION (1)
COLUMN OVEN (1)
COMPUTATIONAL MODELING (1)
CYSTEINE (1)
DAT (1)
DELTA OPIOID RECEPTOR (1)
DERIVATIZATION (1)
DIAGNOSTICS (1)
DIRECT SAMPLING (1)
DOPAMINE TRANSPORTER (1)
DROPLET MICROFLUIDICS (1)
DROSOPHILA (1)
DRUG SCREENING (1)
ELECTROCATALYSIS (1)
ELECTROKINETIC SUPERCHARGING (1)
ELECTROPORATION (1)
ELECTROSPRAY IONISATION (1)
EMPIRICALLY TRANSFORMED GAUSSIAN (1)
EXCITATORY AMINO ACIDS (1)
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Analytical and Bioanalytical Chemistry (8)
Journal of Neuroscience Methods (8)
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Current Opinion in Chemical Biology (3)
European Journal of Neuroscience (3)
Journal of Electroanalytical Chemistry (3)
Journal of Neurochemistry (3)
Analytica Chimica Acta (2)
Journal of Chromatography B (2)
Journal of Chromatography B: Biomedical Sciences and Applications (2)
Journal of The American Society for Mass Spectrometry (2)
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AIChE Journal (1)
Acta Neuropathologica Communications (1)
Alcohol (1)
Angewandte Chemie (1)
Angewandte Chemie International Edition (1)
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Biophysical Journal (1)
Cell Reports (1)
Chinese Chemical Letters (1)
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Data in Brief (1)
ELECTROPHORESIS (1)
Experimental Eye Research (1)
Journal of Separation Science (1)
Journal of the American Society for Mass Spectrometry (1)
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Neuropharmacology (1)
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INFONA - science communication portal

Search results for: Robert T. Kennedy

Mass Activated Droplet Sorting (MADS) Enables High‐Throughput Screening of Enzymatic Reactions at Nanoliter Scale

Mass Activated Droplet Sorting (MADS) Enables High‐Throughput Screening of Enzymatic Reactions at Nanoliter Scale

CE‐MS with electrokinetic supercharging and application to determination of neurotransmitters

Incentive and dopamine sensitization produced by intermittent but not long access cocaine self‐administration

Varying the rate of intravenous cocaine infusion influences the temporal dynamics of both drug and dopamine concentrations in the striatum

Droplet sample introduction to microchip gel and zone electrophoresis for rapid analysis of protein-protein complexes and enzymatic reactions

Detection and quantification of vitamins in microliter volumes of biological samples by LC‐MS for clinical screening

Determination of amines and phenolic acids in wine with benzoyl chloride derivatization and liquid chromatography–mass spectrometry

Pushing the boundaries of chromatography and electrophoresis—Honoring James Jorgenson on his 65th birthday

Microdialysis Coupled with LC-MS/MS for In Vivo Neurochemical Monitoring

Circuit dynamics of in vivo dynorphn release in the nucleus accumbens

Data representing two separate LC-MS methods for detection and quantification of water-soluble and fat-soluble vitamins in tears and blood serum

Age-dependent dopamine transporter dysfunction and Serine129 phospho-α-synuclein overload in G2019S LRRK2 mice

Determination of water-soluble and fat-soluble vitamins in tears and blood serum of infants and parents by liquid chromatography/mass spectrometry

Blunted mGluR Activation Disinhibits Striatopallidal Transmission in Parkinsonian Mice

Design and microfabrication of a miniature fiber optic probe with integrated lenses and mirrors for Raman and fluorescence measurements

Monitoring cell secretions on microfluidic chips using solid-phase extraction with mass spectrometry

Knockdown of ATP citrate lyase in pancreatic beta cells does not inhibit insulin secretion or glucose flux and implicates the acetoacetate pathway in insulin secretion

Repeatability of gradient ultrahigh pressure liquid chromatography–tandem mass spectrometry methods in instrument-controlled thermal environments

Advances in and prospects of microchip liquid chromatography

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