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Random mutations were generated in the G-protein-coupled receptor (Ste2p) for the tridecapeptide pheromone (α-factor) of Saccharomyces cerevisiae. These mutants were screened for variants that responded to antagonists. Because multiple mutations were detected in each mutant receptor recovered from the screen, site-directed mutagenesis was used to create single-site mutant receptors. Three receptors...
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