Search results for: Vladimir Chupin

Items from 1 to 18 out of 18 results

article

Membrane protein targeting into mitochondria

German Legkun, Ivan Okhrimenko, Vladimir Chupin, Valentin Gordeliy

Biochimica et Biophysica Acta (BBA) - Bioenergetics > 2016 > 1857 > S > e125

article

What Makes the Bioactive Lipids Phosphatidic Acid and Lysophosphatidic Acid So Special? ^†

Edgar E. Kooijman, Karen M. Carter, Emma G. van Laar, Vladimir Chupin, more

Biochemistry > 2005 > 44 > 51 > 17007 - 17015

article

A Synergistic Effect between Cholesterol and Tryptophan-Flanked Transmembrane Helices Modulates Membrane Curvature ^†

Bianca Y. van Duyl, Hans Meeldijk, Arie J. Verkleij, Dirk T. S. Rijkers, more

Biochemistry > 2005 > 44 > 11 > 4526 - 4532

article

Polymorphism of Pyridinium Amphiphiles for Gene Delivery: Influence of Ionic Strength, Helper Lipid Content, and Plasmid DNA Complexation

Marco Scarzello, Vladimir Chupin, Anno Wagenaar, Marc C.A. Stuart, more

Biophysical Journal > 2005 > 88 > 3 > 2104-2113

Two double-tailed pyridinium cationic amphiphiles, differing only in the degree of unsaturation of the alkyl chains, have been selected for a detailed study of their aggregation behavior, under conditions employed for transfection experiments. The transfection efficiencies of the two molecules are remarkably different, especially when combined with 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE)...

article

Spontaneous Curvature of Phosphatidic Acid and Lysophosphatidic Acid ^†

Edgar E. Kooijman, Vladimir Chupin, Nola L. Fuller, Michael M. Kozlov, more

Biochemistry > 2005 > 44 > 6 > 2097 - 2102

article

Molecular Organization in Striated Domains Induced by Transmembrane α-Helical Peptides in Dipalmitoyl Phosphatidylcholine Bilayers ^†

Emma Sparr, Dragomir N. Ganchev, Margot M. E. Snel, Anja N. J. A. Ridder, more

Biochemistry > 2005 > 44 > 1 > 2 - 10

article

Molecular Architecture of Nanocapsules, Bilayer-Enclosed Solid Particles of Cisplatin

Vladimir Chupin, Anton I. P. M. de Kroon, Ben de Kruijff

Journal of the American Chemical Society > 2004 > 126 > 42 > 13816 - 13821

article

Small Alcohols Destabilize the KcsA Tetramer via Their Effect on the Membrane Lateral Pressure ^†

Els van den Brink-van der Laan, Vladimir Chupin, J. Antoinette Killian, Ben de Kruijff

Biochemistry > 2004 > 43 > 20 > 5937 - 5942

article

Stability of KcsA Tetramer Depends on Membrane Lateral Pressure ^†

Els van den Brink-van der Laan, Vladimir Chupin, J. Antoinette Killian, Ben de Kruijff

Biochemistry > 2004 > 43 > 14 > 4240 - 4250

article

The Effect of Fructan on the Phospholipid Organization in the Dry State

Ingrid J. Vereyken, Vladimir Chupin, Akhmed Islamov, Alexander Kuklin, more

Biophysical Journal > 2003 > 85 > 5 > 3058-3065

Fructans, a family of oligo- and polyfructoses, are implicated to play a drought-protecting role in plants. Inulin-type fructan is able to preserve the membrane barrier during dehydration. However, whether other fructans would be able to perform this function is unknown. In addition, almost nothing is known about the organization of these systems, which could give insight into the protective mechanism...

article

The Peptide Antibiotic Clavanin A Interacts Strongly and Specifically with Lipid Bilayers ^†

Ellen J. M. van Kan, Dragomir N. Ganchev, Margot M. E. Snel, Vladimir Chupin, more

Biochemistry > 2003 > 42 > 38 > 11366 - 11372

article

Sphingomyelin is much more effective than saturated phosphatidylcholine in excluding unsaturated phosphatidylcholine from domains formed with cholesterol

Bianca Y. van Duyl, Dragomir Ganchev, Vladimir Chupin, Ben de Kruijff, more

FEBS Letters > 2003 > 547 > 1-3 > 101-106

In this study, we compared domain formation in raft-like mixtures of cholesterol and dioleoylphosphatidylcholine (DOPC) with either sphingomyelin (SM) or dipalmitoylphosphatidylcholine (DPPC). Using ² H nuclear magnetic resonance, we studied the properties of the lipid enriched in the fluid phase, DOPC. We found that acyl chain ² H-labeled DOPC is much less ordered in SM-containing...

article

The Effect of Fructan on Membrane Lipid Organization and Dynamics in the Dry State

Ingrid J. Vereyken, Vladimir Chupin, Folkert A. Hoekstra, Sjef C.M. Smeekens, more

Biophysical Journal > 2003 > 84 > 6 > 3759-3766

Fructans are a group of fructose-based oligo- and polysaccharides, which appear to be involved in membrane preservation during dehydration by interacting with the membrane lipids. To get further understanding of the protective mechanism, the consequences of the fructan-membrane lipid interaction for the molecular organization and dynamics in the dry state were studied. POPC and DMPC were investigated...

article

Fructans insert between the headgroups of phospholipids

Ingrid J Vereyken, Vladimir Chupin, Rudy A Demel, Sjef C.M Smeekens, more

Biochimica et Biophysica Acta (BBA) - Biomembranes > 2001 > 1510 > 1-2 > 307-320

Fructans are polysaccharides consisting of one glucose unit and two or more fructose units. It was hypothesized that fructans play a role in drought tolerance in plants by interacting directly with the membrane. In this paper we investigated this hypothesis by studying fructan-membrane interactions in hydrated mono- and bilayer systems. It was found that fructans inserted between the headgroups of...

article

Molecular basis of glycoalkaloid induced membrane disruption

Erik A.J. Keukens, Truus de Vrije, Claudia van den Boom, Pieter de Waard, more

BBA - Biomembranes > 1995 > 1240 > 2 > 216-228

In this study the interaction between the glycoalkaloids α-chaconine, α-solanine and α-tomatine and sterols in model membranes was analysed systematically using techniques like membrane leakage, binding experiments, detergent extraction, electron microscopy, NMR and molecular modelling. The most important properties for sterols to interact with glycoalkaloids turned out to be a planar ring structure...

article

Cardiolipin modulates the secondary structure of the presequence peptide of cytochrome oxidase subunit IV: a 2D ¹ H-NMR study

Vladimir Chupin, Johanna M. Leenhouts, Anton I.P.M. de Kroon, Ben de Kruijff

FEBS Letters > 1995 > 373 > 3 > 239-244

The secondary structure of the presequence of cytochrome oxidase subunit IV (p25) was studied by circular dichroism and 2D nuclear magnetic resonance in micelles of dodecylphosphocholine (DPC) and mixed micelles of DPC and mitochondrial cardiolipin (CL). In both systems, α-helix formation was observed. The α-helix stretches from the N- to the C-terminus with a break at the proline residue at position...

article

The transit sequence of a chloroplast precursor protein reorients the lipids in monogalactosyl diglyceride containing bilayers

Vladimir Chupin, Ron van't Hof, Ben de Kruijff

FEBS Letters > 1994 > 350 > 1 > 104-108

The interaction of the chloroplast precursor protein of ferredoxin with mixed model membranes composed of ² H chain labeled monogalactosyl diacylglycerol and phosphatidylcholine was studied by ² H and ³ ¹ P NMR. The bilayers were found to have special chain packing properties which most likely are the result of a specific arrangement of head groups at the interface. The...

article

A general method for the preparation of mixed micelles of hydrophobic peptides and sodium dodecyl sulphate

J. Antoinette Killian, Theodore P. Trouard, Denise V. Greathouse, Vladimir Chupin, more

FEBS Letters > 1994 > 348 > 2 > 161-165

A new method is reported for the incorporation of hydrophobic peptides into sodium dodecyl sulphate (SDS) micelles. First, a homogeneous solution of peptide and detergent is obtained by adding the peptide in trifluoroethanol to an equal volume of an aqueous solution of SDS. Upon subsequent addition of excess water, mixed peptide-SDS micelles are formed. Next, all solvent is removed by lyophilization...

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