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Site-specific mutagenesis of DNA, developed some thirty years ago, has proven to be one of the most important advances in biology. By allowing the site-specific replacement of any amino acid in a protein with one of the other nineteen amino acids, it ushered in the new era of "Protein Engineering". The field of protein engineering has, however, evolved rapidly since then and the last fifteen...
This article provides a brief summary of Har Gobind Khorana’s early years in science including his years as an independent investigator at the British Columbia Research Council in Vancouver, Canada. It also includes interesting anecdotes about his career and about him as a scientist based on his own writings, including (1) how he simply showed up in the laboratory of Dr. Vladimir Prelog in Zurich,...
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