Search results for: Saul Surrey

Items from 1 to 20 out of 23 results

article

Whole-exome sequencing of DNA from peripheral blood mononuclear cells (PBMC) and EBV-transformed lymphocytes from the same donor

Eric R Londin, Margaret A Keller, Michael R D'Andrea, Kathleen Delgrosso, more

BMC Genomics > 2011 > 12 > 1 > 1-9

Background The creation of lymphoblastoid cell lines (LCLs) through Epstein-Barr virus (EBV) transformation of B-lymphocytes can result in a valuable biomaterial for cell biology research and a renewable source of DNA. While LCLs have been used extensively in cellular and genetic studies, the process of cell transformation and expansion during culturing may introduce genomic changes that may impact...

article

Relationship between β4 hydrogen bond and β6 hydrophobic interactions during aggregate, fiber or crystal formation in oversaturated solutions of hemoglobin A and S

Kazuhiko Adachi, Min Ding, Toshio Asakura, Saul Surrey

Archives of Biochemistry and Biophysics > 2009 > 481 > 2 > 137-144

Oversaturated deoxy-α ₂ β ₂ ^T4V aggregated instantly without a delay time, which is in contrast to the delay time before the generation of fibers of deoxy-HbS and deoxy-α ₂ β ₂ ^E6V,D73H . Solubility of deoxy-α ₂ β ₂ ^T4V was ∼10-fold lower than that of deoxy-HbS and was similar to oxy- and deoxy-α ₂ β ₂ ^E6V,T4V . These...

article

Role of the β4Thr−β73Asp Hydrogen Bond in HbS Polymer and Domain Formation from Multinucleate-Containing Clusters ^†

Kazuhiko Adachi, Min Ding, Saul Surrey

Biochemistry > 2008 > 47 > 19 > 5441 - 5449

article

Assembly of recently translated full-length and C-terminal truncated human γ-globin chains with a pool of α-globin chains to form Hb F in a cell-free system

Kazuhiko Adachi, Yi Zhao, Vinaysagar Lakka, Mitchell J. Weiss, more

Archives of Biochemistry and Biophysics > 2007 > 463 > 1 > 60-67

Assembly of α-globin with translated, full-length and C-terminal truncated human γ-globin to form Hb F was assessed in a cell-free transcription/translation system. Polysome profiles showed two amino acid C-terminal-truncated γ-chains retained on polysomes can assemble with unlabeled holo α-chains only after puromycin-induced chain release. Two amino acid C-terminal truncated γ-chains encoded from...

article

Applications of nanoparticles to diagnostics and therapeutics in colorectal cancer

Paolo Fortina, Larry J Kricka, David J Graves, Jason Park, more

Trends in Biotechnology > 2007 > 25 > 4 > 145-152

Nanotechnology has considerable promise for the detection, staging and treatment of cancer. Here, we outline one such promising application: the use of nanostructures with surface-bound ligands for the targeted delivery and ablation of colorectal cancer (CRC), the third most common malignancy and the second most common cause of cancer-related mortality in the US. Normal colonic epithelial cells as...

article

The Hb A Variant (β73 Asp→Leu) Disrupts Hb S Polymerization by a Novel Mechanism

Kazuhiko Adachi, Min Ding, Saul Surrey, Maria Rotter, more

Journal of Molecular Biology > 2006 > 362 > 3 > 528-538

Polymerization of a 1:1 mixture of hemoglobin S (Hb S) and the artificial mutant HbAβ73Leu produces a dramatic morphological change in the polymer domains in 1.0 M phosphate buffer that are a characteristic feature of polymer formation. Instead of feathery domains with quasi 2-fold symmetry that characterize polymerization of Hb S and all previously known mixtures such as Hb A/S and Hb F/S mixtures,...

article

Inhibition of Hemoglobin S Polymerization in Vitro by a Novel 15-mer EF-Helix β73 Histidine-Containing Peptide ^†

Mohammed G. K. Akbar, Yutaka Tamura, Min Ding, Hua Ding, more

Biochemistry > 2006 > 45 > 27 > 8358 - 8367

article

Nanobiotechnology: the promise and reality of new approaches to molecular recognition

Paolo Fortina, Larry J. Kricka, Saul Surrey, Piotr Grodzinski

Trends in Biotechnology > 2005 > 23 > 4 > 168-173

Nanobiotechnology is the convergence of engineering and molecular biology that is leading to a new class of multifunctional devices and systems for biological and chemical analysis with better sensitivity and specificity and a higher rate of recognition. Nano-objects with important analytical applications include nanotubes, nanochannels, nanoparticles, nanopores and nanocapacitors. Here, we take a...

article

Ethanol potentiates HIV-1 gp120-induced apoptosis in human neurons via both the death receptor and NMDA receptor pathways

Wei Chen, Zhonghua Tang, Paolo Fortina, Pritesh Patel, more

Virology > 2005 > 334 > 1 > 59-73

Neuronal loss is a hallmark of AIDS dementia syndromes. Human immunodeficiency virus type I (HIV-1)-specific proteins may induce neuronal apoptosis, but the signal transduction of HIV-1 gp120-induced, direct neuronal apoptosis remains unclear. Ethanol (EtOH) is considered to be an environmental co-factor in AIDS development. However, whether EtOH abuse in patients with AIDS increases neuronal dysfunction...

article

Ischemic preconditioning-mediated cardioprotection is disrupted in heterozygous Flt-1 (VEGFR-1) knockout mice

Sankar Addya, Keisuke Shiroto, Tibor Turoczi, Lijun Zhan, more

Journal of Molecular and Cellular Cardiology > 2005 > 38 > 2 > 345-351

This study attempts to address an important clinical issue by identifying potential candidates of VEGF signaling through Flt-1 receptor that trigger angiogenic signal under ischemic stress. To determine the significance of VEGF-Flt-1 (VEGFR1) signaling in ischemic preconditioned (PC) myocardium, we used heterozygous Flt-1 knockout (KO) mice to dissect the pathway and identify candidate genes involved...

article

Gene expression profiling during the transition to failure in TNF-α over-expressing mice demonstrates the development of autoimmune myocarditis

Zhonghua Tang, Brian S. McGowan, Sally A. Huber, Charles F. McTiernan, more

Journal of Molecular and Cellular Cardiology > 2004 > 36 > 4 > 515-530

Transgenic mice with cardiac-specific over-expression of tumor necrosis factor-α (TNF1.6) progress to dilated heart failure. A significant inflammatory response precedes functional deterioration, and may contribute to cardiac damage in this model. To evaluate the underlying molecular mechanisms, we assessed the gene expression in six groups of mouse hearts defined by age, gender, and phenotype (n...

article

Significance of β116 His (G18) at α1β1 Contact Sites for αβ Assembly and Autoxidation of Hemoglobin ^†

Kazuhiko Adachi, Yi Yang, Vinaysagar Lakka, Suzanne Wehrli, more

Biochemistry > 2003 > 42 > 34 > 10252 - 10259

article

Effects of heme addition on formation of stable human globin chains and hemoglobin subunit assembly in a cell-free system

Kazuhiko Adachi, Yi Zhao, Saul Surrey

Archives of Biochemistry and Biophysics > 2003 > 413 > 1 > 99-106

Our previous assembly studies to form hemoglobin hetero-dimers and -tetramers using a coupled transcription/translation cell-free system suggested that α-globin chains bind to nascent non-α chains during and/or soon after translation to promote hemoglobin formation [Adachi et al., J. Biol Chem. 2002 (277) 13415]. In this report effects of CN-hemin on subunit assembly were studied using this cell-free...

article

Effects of Different β73 Amino Acids on Formation of 14-Stranded Fibers of Hb S versus Double-Stranded Crystals of Hb C-Harlem ^†

Kazuhiko Adachi, Min Ding, Suzanne Wehrli, Konda S. Reddy, more

Biochemistry > 2003 > 42 > 15 > 4476 - 4484

article

Molecular diagnostics: hurdles for clinical implementation

Paolo Fortina, Saul Surrey, Larry J. Kricka

Trends in Molecular Medicine > 2002 > 8 > 6 > 264-266

In the coming years, molecular diagnostics will continue to be of critical importance to public health worldwide. It will facilitate the detection and characterization of disease, as well as monitoring of the drug response, and will assist in the identification of genetic modifiers and disease susceptibility. A wide range of molecular-based tests is available to assess DNA variation and changes in...

article

GATA and NF-Y Participate in Transcriptional Regulation of FcγRIIA in Megakaryocytic Cells

Diana L Cassel, Sumit K Subudhi, Saul Surrey, Steven E McKenzie

Blood Cells, Molecules and Diseases > 2000 > 26 > 6 > 587-597

ABSTRACT.Human FcγRIIA, expressed on platelets, neutrophils, and macrophages, plays a major role in platelet activation and immune clearance. Clinical observations indicate that regulation of expression of this receptor is an important factor influencing the course of immune thrombocytopenia. We used both transient transfection with FcγRIIA promoter constructs and electrophoretic mobility shift assays...

article

Expression of Functional Soluble Human α-Globin Chains of Hemoglobin in Bacteria

Kazuhiko Adachi, Takamasa Yamaguchi, Yi Yang, Patrick T. Konitzer, more

Protein Expression and Purification > 2000 > 20 > 1 > 37-44

Individual, soluble human α-globin chains were expressed in bacteria with exogenous heme and methionine aminopeptidase. The yields of soluble α chains in bacteria were comparable to those of recombinant non-α chains expressed under the same conditions. Molecular mass and gel-filtration properties of purified recombinant α chains were the same as those of authentic human α chains. Biochemical and biophysical...

article

Effect of Hydrophobicity and Electrostatics on Adsorption and Surface Diffusion of DNA Oligonucleotides at Liquid/Solid Interfaces

Vincent Chan, Steven E. McKenzie, Saul Surrey, Paolo Fortina, more

Journal of Colloid And Interface Science > 1998 > 203 > 1 > 197-207

It is generally known that both electrostatic and hydrophobic interactions are major driving forces for protein adsorption at solid/liquid interfaces, but relatively little is known about how these interactions affect the interfacial behavior of single-stranded DNA molecules. This information is important for the design of DNA hybridization sensors. In this study, total internal reflectance microscopy/fluorescence...

article

Connexin-26 mutations in sporadic and inherited sensorineural deafness

Xavier Estivill, Paolo Fortina, Saul Surrey, Raquel Rabionet, more

The Lancet > 1998 > 351 > 9100 > 394-398

Hearing impairment affects one infant in 1000 and 4% of people aged younger than 45 years. Congenital deafness is inherited or apparently sporadic. We have shown previously that DFNB1 on chromosome 13 is a major locus for recessive deafness in about 80% of Mediterranean families and that the connexin-26 gene gap junction protein β2 (GJB2) is mutated in DFNB1 families. We investigated mutations in...

article

Rapid sizing of polymorphic microsatellite markers by capillary array electrophoresis

Elaine S. Mansfield, Marina Vainer, Dennis W. Harris, Paolo Gasparini, more

Journal of Chromatography A > 1997 > 781 > 1-2 > 295-305

Genetic mapping and DNA sequencing projects could potentially be completed more rapidly by using capillary array electrophoresis (CAE) systems running 48-96 capillaries simultaneously. Currently, multiplex polymerase chain reaction (PCR) and multicolor fluorescent dye-labeling strategies are used to generate DNA profiles containing 18-24 genotypes per sample. By using 4-color fluorescence detection...

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Search results for: Saul Surrey

Whole-exome sequencing of DNA from peripheral blood mononuclear cells (PBMC) and EBV-transformed lymphocytes from the same donor

Relationship between β4 hydrogen bond and β6 hydrophobic interactions during aggregate, fiber or crystal formation in oversaturated solutions of hemoglobin A and S

Role of the β4Thr−β73Asp Hydrogen Bond in HbS Polymer and Domain Formation from Multinucleate-Containing Clusters ^†

Assembly of recently translated full-length and C-terminal truncated human γ-globin chains with a pool of α-globin chains to form Hb F in a cell-free system

Applications of nanoparticles to diagnostics and therapeutics in colorectal cancer

The Hb A Variant (β73 Asp→Leu) Disrupts Hb S Polymerization by a Novel Mechanism

Inhibition of Hemoglobin S Polymerization in Vitro by a Novel 15-mer EF-Helix β73 Histidine-Containing Peptide ^†

Nanobiotechnology: the promise and reality of new approaches to molecular recognition

Ethanol potentiates HIV-1 gp120-induced apoptosis in human neurons via both the death receptor and NMDA receptor pathways

Ischemic preconditioning-mediated cardioprotection is disrupted in heterozygous Flt-1 (VEGFR-1) knockout mice

Gene expression profiling during the transition to failure in TNF-α over-expressing mice demonstrates the development of autoimmune myocarditis

Significance of β116 His (G18) at α1β1 Contact Sites for αβ Assembly and Autoxidation of Hemoglobin ^†

Effects of heme addition on formation of stable human globin chains and hemoglobin subunit assembly in a cell-free system

Effects of Different β73 Amino Acids on Formation of 14-Stranded Fibers of Hb S versus Double-Stranded Crystals of Hb C-Harlem ^†

Molecular diagnostics: hurdles for clinical implementation

GATA and NF-Y Participate in Transcriptional Regulation of FcγRIIA in Megakaryocytic Cells

Expression of Functional Soluble Human α-Globin Chains of Hemoglobin in Bacteria

Effect of Hydrophobicity and Electrostatics on Adsorption and Surface Diffusion of DNA Oligonucleotides at Liquid/Solid Interfaces

Connexin-26 mutations in sporadic and inherited sensorineural deafness

Rapid sizing of polymorphic microsatellite markers by capillary array electrophoresis

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