Search results for: Ivan Rayment

Items from 1 to 20 out of 52 results

article

Structural and functional analysis of an l‐serine O‐phosphate decarboxylase involved in norcobamide biosynthesis

Sebastian Keller, Karl M. Wetterhorn, Alison Vecellio, Mark Seeger, more

Structural diversity of natural cobamides (Cbas, B₁₂ vitamers) is limited to the nucleotide loop. The loop is connected to the cobalt‐containing corrin ring via an (R)‐1‐aminopropan‐2‐ol O‐2‐phosphate (AP‐P) linker moiety. AP‐P is produced by the l‐threonine O‐3‐phosphate (l‐Thr‐P) decarboxylase CobD. Here, the CobD homolog SMUL_1544 of the organohalide‐respiring epsilonproteobacterium Sulfurospirillum multivorans...

article

Reversible swelling of SBMV is associated with reversible disordering

Jade Li, Carl Fricks, Ivan Rayment, Donald L.D. Caspar

Journal of Structural Biology > 2017 > 200 > 3 > 314-324

The structures of the compact and swollen southern bean mosaic virus (SBMV) particles have been compared by X-ray diffraction and proton magnetic resonance (PMR). Small-angle X-ray scattering showed that removal of divalent cations at alkaline pH causes the particle diameter to increase from 289Å in the native SBMV by 12% in solution and by 9% in microcrystals. The swelling is fully reversible upon...

article

Design considerations in coiled-coil fusion constructs for the structural determination of a problematic region of the human cardiac myosin rod

Michael P. Andreas, Gautam Ajay, Jaclyn A. Gellings, Ivan Rayment

Journal of Structural Biology > 2017 > 200 > 3 > 219-228

X-ray structural determination of segments of the myosin rod has proved difficult because of the strong salt-dependent aggregation properties and repeating pattern of charges on the surface of the coiled-coil that lead to the formation of paracrystals. This problem has been resolved in part through the use of globular assembly domains that improve protein folding and prevent aggregation. The primary...

article

Functional characterization of a soluble NADPH-cytochrome P450 reductase from Fusarium graminearum

Thomas Etzerodt, Karl Wetterhorn, Giuseppe Dionisio, Ivan Rayment

Protein Expression and Purification > 2017 > 138 > C > 69-75

Fusarium head blight is a devastating disease in wheat caused by some fungal pathogens of the Fusarium genus mainly F. graminearum, due to accumulation of toxic trichothecenes. Most of the trichothecene biosynthetic pathway has been mapped, although some proteins of the pathway remain uncharacterized, including an NADPH-cytochrome P450 reductase. We subcloned a F. graminearum cytochrome P450 reductase...

article

A composite approach towards a complete model of the myosin rod

E. Nihal Korkmaz, Keenan C. Taylor, Michael P. Andreas, Guatam Ajay, more

Proteins: Structure, Function, and Bioinformatics > 84 > 1 > 172 - 189

Sarcomeric myosins have the remarkable ability to form regular bipolar thick filaments that, together with actin thin filaments, constitute the fundamental contractile unit of skeletal and cardiac muscle. This has been established for over 50 years and yet a molecular model for the thick filament has not been attained. In part this is due to the lack of a detailed molecular model for the coiled‐coil...

article

Kinesin-2 KIF3AC and KIF3AB Can Drive Long-Range Transport along Microtubules

Stephanie Guzik-Lendrum, Katherine C. Rank, Brandon M. Bensel, Keenan C. Taylor, more

Biophysical Journal > 2015 > 109 > 7 > 1472-1482

Mammalian KIF3AC is classified as a heterotrimeric kinesin-2 that is best known for organelle transport in neurons, yet in vitro studies to characterize its single molecule behavior are lacking. The results presented show that a KIF3AC motor that includes the native helix α7 sequence for coiled-coil formation is highly processive with run lengths of ∼1.23 μm and matching those exhibited by conventional...

article

Why are Kinesin-2 KIF3AB and KIF3AC so Processive?

Stephanie Guzik-Lendrum, Katherine C. Rank, Brandon Bensel, Ivan Rayment, more

Biophysical Journal > 2015 > 108 > 2 Supplement 1 > 21a

article

Dissecting cobamide diversity through structural and functional analyses of the base-activating CobT enzyme of Salmonella enterica

Chi Ho Chan, Sean A. Newmister, Keenan Talyor, Kathy R. Claas, more

BBA - General Subjects > 2014 > 1840 > 1 > 464-475

Cobamide diversity arises from the nature of the nucleotide base. Nicotinate mononucleotide (NaMN):base phosphoribosyltransferases (CobT) synthesize α-linked riboside monophosphates from diverse nucleotide base substrates (e.g., benzimidazoles, purines, phenolics) that are incorporated into cobamides.Structural investigations of two members of the CobT family of enzymes in complex with various substrate...

article

Common mechanistic themes for the powerstroke of kinesin-14 motors

Miguel A. Gonzalez, Julia Cope, Katherine C. Rank, Chun Ju Chen, more

Journal of Structural Biology > 2013 > 184 > 2 > 335-344

Kar3Cik1 is a heterodimeric kinesin-14 from Saccharomyces cerevisiae involved in spindle formation during mitosis and karyogamy in mating cells. Kar3 represents a canonical kinesin motor domain that interacts with microtubules under the control of ATP-hydrolysis. In vivo, the localization and function of Kar3 is differentially regulated by its interacting stoichiometrically with either Cik1 or Vik1,...

article

Functional asymmetry in kinesin and dynein dimers

Katherine C. Rank, Ivan Rayment

Biology of the Cell > 105 > 1 > 1 - 13

Active transport along the microtubule lattice is a complex process that involves both the Kinesin and Dynein superfamily of motors. Transportation requires sophisticated regulation much of which occurs through the motor's tail domain. However, a significant portion of this regulation also occurs through structural changes that arise in the motor and the microtubule upon binding. The most obvious...

article

Structure and Mutational Analysis of the Archaeal GTP:AdoCbi-P Guanylyltransferase (CobY) from Methanocaldococcus jannaschii: Insights into GTP Binding and Dimerization

Sean A. Newmister, Michele M. Otte, Jorge C. Escalante-Semerena, Ivan Rayment

Biochemistry > 2011 > 50 > 23 > 5301 - 5313

article

Structure of the Tropomyosin Overlap Complex from Chicken Smooth Muscle: Insight into the Diversity of N-Terminal Recognition,

Jeremiah Frye, Vadim A. Klenchin, Ivan Rayment

Biochemistry > 2010 > 49 > 23 > 4908 - 4920

article

Insights into the Importance of Hydrogen Bonding in the γ-Phosphate Binding Pocket of Myosin: Structural and Functional Studies of Serine 236,

Jeremiah J. Frye, Vadim A. Klenchin, Clive R. Bagshaw, Ivan Rayment

Biochemistry > 2010 > 49 > 23 > 4897 - 4907

article

Cryo-electron tomography of microtubule–kinesin motor complexes

Julia Cope, Susan Gilbert, Ivan Rayment, David Mastronarde, more

Journal of Structural Biology > 2010 > 170 > 2 > 257-265

Microtubules complexed with molecular motors of the kinesin family or non-motor microtubule associated proteins (MAPs) such as tau or EB1 have been the subject of cryo-electron microcopy based 3-D studies for several years. Most of these studies that targeted complexes with intact microtubules have been carried out by helical 3-D reconstruction, while few were analyzed by single particle approaches...

article

Residue Phe112 of the Human-Type Corrinoid Adenosyltransferase (PduO) Enzyme of Lactobacillus reuteri Is Critical to the Formation of the Four-Coordinate Co(II) Corrinoid Substrate and to the Activity of the Enzyme ^† , ^‡

Paola E. Mera, Martin St Maurice, Ivan Rayment, Jorge C. Escalante-Semerena

Biochemistry > 2009 > 48 > 14 > 3138 - 3145

article

Structural Characterization of a Human-Type Corrinoid Adenosyltransferase Confirms That Coenzyme B ₁₂ Is Synthesized through a Four-Coordinate Intermediate ^† ^‡

Martin St. Maurice, Paola Mera, Kiyoung Park, Thomas C. Brunold, more

Biochemistry > 2008 > 47 > 21 > 5755 - 5766

article

Structural and Functional Analyses of the Human-Type Corrinoid Adenosyltransferase (PduO) from Lactobacillus reuteri ^† ^, ^‡

Paola E. Mera, Martin St. Maurice, Ivan Rayment, Jorge C. Escalante-Semerena

Biochemistry > 2007 > 46 > 48 > 13829 - 13836

article

A Structural Basis for Regulation of Actin Polymerization by Pectenotoxins

John S. Allingham, Christopher O. Miles, Ivan Rayment

Journal of Molecular Biology > 2007 > 371 > 4 > 959-970

(PTXs) are polyether macrolides found in certain dinoflagellates, sponges and shellfish, and have been associated with diarrhetic shellfish poisoning. In addition to their in vivo toxicity, some PTXs are potently cytotoxic in human cancer cell lines. Recent studies have demonstrated that disruption of the actin cytoskeleton may be a key function of these compounds, although no clarification of their...

article

Vik1 Modulates Microtubule-Kar3 Interactions through a Motor Domain that Lacks an Active Site

John S. Allingham, Lisa R. Sproul, Ivan Rayment, Susan P. Gilbert

Cell > 2007 > 128 > 6 > 1161-1172

Conventional kinesin and class V and VI myosins coordinate the mechanochemical cycles of their motor domains for processive movement of cargo along microtubules or actin filaments. It is widely accepted that this coordination is achieved by allosteric communication or mechanical strain between the motor domains, which controls the nucleotide state and interaction with microtubules or actin. However,...

article

Crystal Structure of Polymerization-Competent Actin

Vadim A. Klenchin, Sofia Y. Khaitlina, Ivan Rayment

Journal of Molecular Biology > 2006 > 362 > 1 > 140-150

All actin crystal structures reported to date represent actin complexed or chemically modified with molecules that prevent its polymerization. Actin cleaved with ECP32 protease at a single site between Gly42 and Val43 is virtually non-polymerizable in the Ca-ATP bound form but remains polymerization-competent in the Mg-bound form. Here, a crystal structure of the true uncomplexed ECP32-cleaved actin...

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Search results for: Ivan Rayment

Structural and functional analysis of an l‐serine O‐phosphate decarboxylase involved in norcobamide biosynthesis

Reversible swelling of SBMV is associated with reversible disordering

Design considerations in coiled-coil fusion constructs for the structural determination of a problematic region of the human cardiac myosin rod

Functional characterization of a soluble NADPH-cytochrome P450 reductase from Fusarium graminearum

A composite approach towards a complete model of the myosin rod

Kinesin-2 KIF3AC and KIF3AB Can Drive Long-Range Transport along Microtubules

Why are Kinesin-2 KIF3AB and KIF3AC so Processive?

Dissecting cobamide diversity through structural and functional analyses of the base-activating CobT enzyme of Salmonella enterica

Common mechanistic themes for the powerstroke of kinesin-14 motors

Functional asymmetry in kinesin and dynein dimers

Structure and Mutational Analysis of the Archaeal GTP:AdoCbi-P Guanylyltransferase (CobY) from Methanocaldococcus jannaschii: Insights into GTP Binding and Dimerization

Structure of the Tropomyosin Overlap Complex from Chicken Smooth Muscle: Insight into the Diversity of N-Terminal Recognition,

Insights into the Importance of Hydrogen Bonding in the γ-Phosphate Binding Pocket of Myosin: Structural and Functional Studies of Serine 236,

Cryo-electron tomography of microtubule–kinesin motor complexes

Residue Phe112 of the Human-Type Corrinoid Adenosyltransferase (PduO) Enzyme of Lactobacillus reuteri Is Critical to the Formation of the Four-Coordinate Co(II) Corrinoid Substrate and to the Activity of the Enzyme ^† , ^‡

Structural Characterization of a Human-Type Corrinoid Adenosyltransferase Confirms That Coenzyme B ₁₂ Is Synthesized through a Four-Coordinate Intermediate ^† ^‡

Structural and Functional Analyses of the Human-Type Corrinoid Adenosyltransferase (PduO) from Lactobacillus reuteri ^† ^, ^‡

A Structural Basis for Regulation of Actin Polymerization by Pectenotoxins

Vik1 Modulates Microtubule-Kar3 Interactions through a Motor Domain that Lacks an Active Site

Crystal Structure of Polymerization-Competent Actin

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