Search results for: Andy C. LiWang

Items from 1 to 13 out of 13 results

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¹H, ¹³C and ¹⁵N Chemical Shift Assignments of the C-Terminal, 133-Residue Pseudo-Receiver Domain of Circadian Input Kinase (CikA) in Synechococcus elongatus

Tiyu Gao, Xiaofan Zhang, Youlin Xia, Yoonsang Cho, more

Journal of Biomolecular NMR > 2005 > 32 > 3 > 259-259

Electronic Supplementary Material Electronic Supplementary material is available for this article at http://dx.doi.org/10.1007/s10858-005-7944-6 and accessible for authorised users.

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Structure of the N-terminal Domain of the Circadian Clock-associated Histidine Kinase SasA

Ioannis Vakonakis, Douglas A. Klewer, Stanly B. Williams, Susan S. Golden, more

Journal of Molecular Biology > 2004 > 342 > 1 > 9-17

Circadian oscillators are endogenous biological systems that generate the ∼24 hour temporal pattern of biological processes and confer a reproductive fitness advantage to their hosts. The cyanobacterial clock is the simplest known and the only clock system for which structural information for core component proteins, in this case KaiA, KaiB and KaiC, is available. SasA, a clock-associated histidine...

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N1···N3 Hydrogen Bonds of A:U Base Pairs of RNA Are Stronger than Those of A:T Base Pairs of DNA [ J. Am. Chem. Soc. 2004 , 18 , 5688−5689].

Ioannis Vakonakis, Andy C. LiWang

Journal of the American Chemical Society > 2004 > 126 > 22 > 7152 - 7152

article

N1···N3 Hydrogen Bonds of A:U Base Pairs of RNA Are Stronger than Those of A:T Base Pairs of DNA

Ioannis Vakonakis, Andy C. LiWang

Journal of the American Chemical Society > 2004 > 126 > 18 > 5688 - 5689

article

Letter to the Editor: Sequence-specific ¹H, ¹³C and ¹⁵N resonance assignments of the C-terminal domain of KaiA, a circadian clock protein

Ioannis Vakonakis, Andy C. LiWang

Journal of Biomolecular NMR > 2004 > 28 > 4 > 403-404

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Trans-hydrogen bond deuterium isotope effects of A:T base pairs in DNA

Ioannis Vakonakis, Andy C. LiWang

Journal of Biomolecular NMR > 2004 > 29 > 1 > 65-72

The chemical shifts of ¹³C2 of adenosine residues of DNA were observed to experience a through-space or trans-hydrogen bond isotope effect as a result of deuterium substitution at the imino hydrogen site of base-paired thymidine residues. NMR measurements of several self-complementary DNA duplexes at natural abundance ¹³C in 50% H₂O, 50% D₂O solvent mixtures yielded an average trans-hydrogen bond...

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Observation of a distinct transition in the mode of interconversion of ring pucker conformers in non-crystalline d-ribose-2′-d from ²H NMR spin-alignment

Andy C. LiWang, David E. McCready, Gary P. Drobny, Brian R. Reid, more

Journal of Biomolecular NMR > 2003 > 26 > 3 > 249-257

Internal motions of d-ribose selectively ²H-labeled at the 2^′ position were measured using solid state ²H NMR experiments. A sample of d-ribose-2 ^′-d was prepared in a hydrated, non-crystalline state to eliminate effects of crystal-packing. Between temperatures of −74 and −60 °C the C2^′–H2^′ bond was observed to undergo two kinds of motions which were similar to those of C2^′–H2^′/H2^′′ found previously...

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Deuterium isotope effects and fractionation factors of hydrogen-bonded A:T base pairs of DNA

Ioannis Vakonakis, Miguel Salazar, Mijeong Kang, Kim R. Dunbar, more

Journal of Biomolecular NMR > 2003 > 25 > 2 > 105-112

Deuterium isotope effects and fractionation factors of N1...H3–N3 hydrogen bonded Watson–Crick A:T base pairs of two DNA dodecamers are presented here. Specifically, two-bond deuterium isotope effects on the chemical shifts of ¹³C2 and ¹³C4, ²Δ¹³C2 and ²Δ¹³C4, and equilibrium deuterium/protium fractionation factors of H3, Φ, were measured and seen to correlate with the chemical shift of the corresponding...

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Letter to the Editor: Sequence-specific resonance assignments of the N-terminal, 105-residue KaiC-interacting domain of SasA, a protein necessary for a robust circadian rhythm in Synechococcus elongatus

Douglas A. Klewer, Stanly B. Williams, Susan S. Golden, Andy C. LiWang

Journal of Biomolecular NMR > 2002 > 24 > 1 > 77-78

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Conformations of Nucleoside Analogue 1-(2‘-Deoxy-β- d -ribofuranosyl)-1,2,4-triazole-3-carboxamide in Different DNA Sequence Contexts ^†

Douglas A. Klewer, Peiming Zhang, Donald E. Bergstrom, V. Jo Davisson, more

Biochemistry > 2001 > 40 > 6 > 1518 - 1527

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Letter to the Editor: Sequence-specific 1H, 13C and 15N resonance assignments of the N-terminal, 135-residue domain of KaiA, a clock protein from Synechococcus elongatus

Ioannis Vakonakis, Aaron T. Risinger, Michael P. Latham, Stanly B. Williams, more

Journal of Biomolecular NMR > 2001 > 21 > 2 > 179-180

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Solution NMR Characterization of Hydrogen Bonds in a Protein by Indirect Measurement of Deuterium Quadrupole Couplings

Andy C Liwang, Ad Bax

Journal of Magnetic Resonance > 1997 > 127 > 1 > 54-64

Hydrogen bonds stabilize protein and nucleic acid structure, but little direct spectroscopic data have been available for characterizing these critical interactions in biological macromolecules. It is demonstrated that the electric field gradient at the nucleus of an amide hydrogen can be determined residue-specific by measurement of ¹⁵ N NMR relaxation times in proteins dissolved in D ...