Search results for: J. Bruce Pitner

Items from 1 to 8 out of 8 results

article

Multi-day pre-clinical demonstration of glucose/galactose binding protein-based fiber optic sensor

Kristin Weidemaier, Alexander Lastovich, Steven Keith, J. Bruce Pitner, more

Biosensors and Bioelectronics > 2011 > 26 > 10 > 4117-4123

We report here the first pre-clinical demonstration of continuous glucose tracking by fluorophore-labeled and genetically engineered glucose/galactose binding protein (GGBP). Acrylodan-labeled GGBP was immobilized in a hydrogel matrix at the tip of a small diameter optical fiber contained in a stainless steel needle. The fiber optic biosensors were inserted subcutaneously into Yucatan and Yorkshire...

article

Synthesis and Biosensor Performance of a Near-IR Thiol-Reactive Fluorophore Based on Benzothiazolium Squaraine

Joseph Thomas, Douglas B. Sherman, Terry J. Amiss, Sandra A. Andaluz, more

Bioconjugate Chemistry > 2007 > 18 > 6 > 1841 - 1846

article

Synthesis of Thiol-Reactive, Long-Wavelength Fluorescent Phenoxazine Derivatives for Biosensor Applications

Douglas B. Sherman, J. Bruce Pitner, Arounaguiry Ambroise, K. Joseph Thomas

Bioconjugate Chemistry > 2006 > 17 > 2 > 387 - 392

article

Direct detection of glucose by surface plasmon resonance with bacterial glucose/galactose-binding protein

Helen V. Hsieh, Zachary A. Pfeiffer, Terry J. Amiss, Douglas B. Sherman, more

Biosensors and Bioelectronics > 2004 > 19 > 7 > 653-660

The monitoring and management of blood glucose levels are key components for maintaining the health of people with diabetes. Traditionally, glucose monitoring has been based on indirect detection using electrochemistry and enzymes such as glucose oxidase or glucose dehydrogenase. Here, we demonstrate direct detection of glucose using a surface plasmon resonance (SPR) biosensor. By site-specifically...

article

Using Receptor Conformational Change To Detect Low Molecular Weight Analytes by Surface Plasmon Resonance

Jason E. Gestwicki, Helen V. Hsieh, J. Bruce Pitner

Analytical Chemistry > 2001 > 73 > 23 > 5732 - 5737

article

Bivalency and epitope specificity of a high-affinity IgG3 monoclonal antibody to the Streptococcus Group A carbohydrate antigen. Molecular modeling of a Fv fragment

J. Bruce Pitner, Wayne F. Beyer, Thomas M. Venetta, Colleen Nycz, more

Carbohydrate Research > 2000 > 324 > 1 > 17 - 29

The binding of Strep 9, a mouse monoclonal antibody (mAb) of the IgG3 subclass directed against the cell-wall polysaccharide of Group A Streptococcus (GAS), has been characterized. The intact antibody and proteolytic fragments of Strep 9 bind differently to GAS: the intact mAb and F(ab)' ₂ have greater affinity for the carbohydrate epitope than the monomeric Fab or F(ab)'. A mode of binding...

article

Real-Time, Sequence-Specific Detection of Nucleic Acids during Strand Displacement Amplification

James G. Nadeau, J. Bruce Pitner, C. Preston Linn, James L. Schram, more

Analytical Biochemistry > 1999 > 276 > 2 > 177-187

Strand displacement amplification (SDA) is an isothermal nucleic acid amplification method based on the primer-directed nicking activity of a restriction enzyme and the strand displacement activity of an exonuclease-deficient polymerase. Here we describe fluorogenic reporter probes that permit real-time, sequence-specific detection of targets amplified during SDA. The new probes possess the single-strand...

article

Covalent cyanine dye oligonucleotide conjugates

C Preston Linn, J Bruce Pitner, Pat D Mize

Biotechnology Advances > 1997 > 15 > 3-4 > 718

The present invention relates to conjugates of a cyanine dye and an oligonucleotide. When these conjugates hybridize or bind to a target, a detectable increase in fluorescence intensity or change in fluorescence polarization is observed.

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Search results for: J. Bruce Pitner

Multi-day pre-clinical demonstration of glucose/galactose binding protein-based fiber optic sensor

Synthesis and Biosensor Performance of a Near-IR Thiol-Reactive Fluorophore Based on Benzothiazolium Squaraine

Synthesis of Thiol-Reactive, Long-Wavelength Fluorescent Phenoxazine Derivatives for Biosensor Applications

Direct detection of glucose by surface plasmon resonance with bacterial glucose/galactose-binding protein

Using Receptor Conformational Change To Detect Low Molecular Weight Analytes by Surface Plasmon Resonance

Bivalency and epitope specificity of a high-affinity IgG3 monoclonal antibody to the Streptococcus Group A carbohydrate antigen. Molecular modeling of a Fv fragment

Real-Time, Sequence-Specific Detection of Nucleic Acids during Strand Displacement Amplification

Covalent cyanine dye oligonucleotide conjugates

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