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Probing of molecular oxygen in mammalian cells is important for the analysis of mitochondrial function, metabolic responses, and energetic status of the cells. We describe a new panel of intracellular O 2 -sensitive probes based on phosphorescent porphyrin dyes conjugated to cell-penetrating peptides. The probes comprising the uncharged derivatives of Pt(II)-coproporphyrin I covalently linked...
Cellular oxygen uptake is an informative parameter of cellular function but is not measured routinely in the analysis of cytotoxicity. Here we have evaluated the ability of a fluorescence-based oxygen uptake assay to assess the metabolic activity of common adherent cells including HepG2, LLC-PK1, Hek293T, C2C12, H-4-II-E, and primary rat hepatocytes. The assay employs water-soluble phosphorescent...
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