Search results for: Sabrina Deroo

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article

Neutralising properties of peptides derived from CXCR4 extracellular loops towards CXCL12 binding and HIV-1 infection

Andy Chevigné, Virginie Fievez, Martyna Szpakowska, Aurélie Fischer, more

BBA - Molecular Cell Research > 2014 > 1843 > 5 > 1031-1041

The chemokine receptor CXCR4 interacts with a single endogenous chemokine, CXCL12, and regulates a wide variety of physiological and pathological processes including inflammation and metastasis development. CXCR4 also binds the HIV-1 envelope glycoprotein, gp120, resulting in viral entry into host cells. Therefore, CXCR4 and its ligands represent valuable drug targets. In this study, we investigated...

article

Engineering and screening the N-terminus of chemokines for drug discovery

Andy Chevigné, Virginie Fievez, Jean-Claude Schmit, Sabrina Deroo

Biochemical Pharmacology > 2011 > 82 > 10 > 1438-1456

Chemokines are small chemoattractive proteins involved in many important physiological and pathological processes such as leukocyte mobilisation, inflammation, cancer and HIV-1 infection. The N-terminus of chemokines was shown to be crucial for interaction and activation with their cognate receptors. Therefore, multiple strategies including elongation, truncation, mutagenesis or chemical modifications...

article

Selection of a CXCR4 antagonist from a human heavy chain CDR3‐derived phage library

Andy Chevigné, Aurélie Fischer, Julie Mathu, Manuel Counson, more

The FEBS Journal > 278 > 16 > 2867 - 2878

Phage display technology is a powerful selection approach to identify strong and specific binders to a large variety of targets. In this study, we compared the efficacy of a phage library displaying human heavy chain complementarity determining region 3 (HCDR3) repertoires with a set of conventional random peptide libraries for the identification of CXCR4 antagonists using a peptide corresponding...

article

Non-immunized natural human heavy chain CDR3 repertoires allow the isolation of high affinity peptides mimicking a human influenza hemagglutinin epitope

Sabrina Deroo, Aurélie Fischer, Nadia Beaupain, Manuel Counson, more

Molecular Immunology > 2008 > 45 > 5 > 1366-1373

In this study we constructed two phage libraries displaying non-immunized natural human IgM derived HCDR3 repertoires. One library was structurally constrained by a Gly to Cys substitution at position 104 enabling the formation of a disulfide bridge with the Cys at position 92. Panning of these libraries on an anti-human influenza hemagglutinin (HA) antibody resulted in the selection of 16 different...

article

Synthesis of 4-[2-Aminoethyl(nitrosamino)]-1-pyridin-3-yl-butan-1-one, a New NNK Hapten for the Induction of N -Nitrosamine-Specific Antibodies

Emmanuel J. F. Prodhomme, Corinne Ensch, Fabienne B. Bouche, Thomas Kaminski, more

Bioconjugate Chemistry > 2007 > 18 > 6 > 2045 - 2053

article

A new recombinant virus system for the study of HIV-1 entry and inhibition

Francois Roman, Wim Ammerlaan, Jean-Marc Plesseria, Sabrina Deroo, more

Journal of Virological Methods > 2006 > 131 > 1 > 99-104

The construction is described of a HIV-1 proviral, eGFP-tagged plasmid that allows for the recombination of any selected env gene without the use of restriction enzymes and for the quantitation of the infection by the recombinant virus using flow cytometry. The system was tested showing that an isoleucine to valine substitution at residue position 37 of the HIV-1 gp41 impairs the fitness of the virus...

article

Heteroduplex mobility assay (HMA) pre-screening: An improved strategy for the rapid identification of inserts selected from phage-displayed peptide libraries

Fred Fack, Sabrina Deroo, Stephanie Kreis, Claude P. Muller

Molecular Diversity > 2000 > 5 > 1 > 7-12

Phage-displayed peptide libraries represent an efficient toolto isolate peptides that bind a given target molecule. Afterseveral selection rounds, generally a large pool of targetbinding phages is obtained. Conventional analysis of theselected phage population involves extensive sequencing of many clones, mostof which can be identical. We have adapted the HeteroduplexMobility Assay (HMA) for pre-screening...

article

Crossreactivity of mimotopes and peptide homologues of a sequential epitope with a monoclonal antibody does not predict crossreactive immunogenicity

Karim C. El Kasmi, Sabrina Deroo, Dietmar M. Theisen, Nicolaas H.C. Brons, more

Vaccine > 1999 > 18 > 3-4 > 284-290

The sequence H236-256 of the measles virus (MV) hemagglutinin (H) contains the sequential epitope of the neutralizing and protective monoclonal antibody (mAb) BH129 with the minimal epitope E ₂ ₄ ₅ L-QL ₂ ₄ ₉ . Using this mAb, we have recently developed 7mer mimotopes binding up to 135x better than the corresponding 7mer epitope H244-250. In this study, we combined...

article

Enhanced antigenicity of a four-contact-residue epitope of the measles virus hemagglutinin protein by phage display libraries: evidence of a helical structure in the putative active site

Sabrina Deroo, Karim C. El Kasmi, Philippe Fournier, Dietmar Theisen, more

Molecular Immunology > 1998 > 35 > 8 > 435-443

Antigenicity and conformational propensities of synthetic peptides corresponding to the sequential epitope H236-255 of the measles virus hemagglutinin protein were investigated. This epitope corresponds to the neutralising and protective monoclonal antibody BH129 and includes Arg243, implicated in CD46-down-regulation and Arg253 that has been mapped to the putative enzymatic site. Fine mapping with...

article

Phage displayed 6-mer mimotopes with a consensus proline absent in the minimized linear wild-type epitope

Sabrina Deroo, Philippe Fournier, Dietmar Theisen, Nicolas H.C. Brons, more

International Journal of Peptide Research and Therapeutics > 1998 > 5 > 2-3 > 159-162

Phage displayed random 6-mer libraries were screened with a monoclonal antibody specific for a minimized ‘linear’ 7-mer epitope of the measles virus hemagglutinin protein. No clone with the wild-type sequence was selected and most clones contained a sequence motif not found in the wild-type sequence. Two mimotopes (LYMPQLS, SEMPQLP) were synthesized which inhibited binding to the measles virus 95–135...

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Search results for: Sabrina Deroo

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