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PEG intrazellulär! Obwohl PEGylierungen die biologische Halbwertszeit von Peptiden in Serum und Lysat verlängern, wurde der Einfluss der PEGylierung auf das Verhalten biologischer Wirkstoffe in Zellen bis jetzt kaum untersucht. Hier wird gezeigt, dass Phosphoramidat‐verzweigte PEGylierte proapoptotische Peptide eine erhöhte Stabilität in Jurkat‐Zelllysat aufweisen, homogen in der Zelle verteilt sind...
Fluorescence correlation spectroscopy (FCS) derives information on molecular interactions and concentrations from the analysis of timedependent fluctuations of a fluorescence signal, caused by diffusion of molecules through a confocal detection volume. Here, we present applications of FCS in drug discovery and molecular pharmacology, including the screening of drug-target interactions and peptide...
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