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The cloning, expression in vitro, and characterization of two aminopeptidase Ns (APN5s and APN2s) isolated from the midgut of Cry1Ac‐resistant (R) and susceptible (S) strains of Plutella xylostella larvae are presented in this paper. The deduced amino acid sequences of APN5s included C‐terminal GPI‐modification sites, the gluzincin aminopeptidase motif GATEN, and three N‐glycosylated sites; those...
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