Search results for: Pietro Pucci

Items from 1 to 16 out of 16 results

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Spectroscopic investigation of auranofin binding to zinc finger HIV-2 nucleocapsid peptides

Maria Antonietta Castiglione Morelli, Angela Ostuni, Giorgio Matassi, Camilla Minichino, more

Inorganica Chimica Acta > 2016 > 453 > C > 330-338

The nucleocapsidic protein (NC) of orthoretroviruses has been considered as a promising target for antiretroviral drugs. Compounds, including zinc ejectors and metal derivatives capable to substitute the zinc ion in the nucleocapsidic zinc fingers, have been described. Auranofin, a gold(I) drug used for the treatment of rheumatoid arthritis, restricts the viral reservoir in the monkey AIDS model and...

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Identification of major Toxoneuron nigriceps venom proteins using an integrated transcriptomic/proteomic approach

Simona Laurino, Gerarda Grossi, Pietro Pucci, Angela Flagiello, more

Insect Biochemistry and Molecular Biology > 2016 > 76 > C > 49-61

Endoparasitoids in the order Hymenoptera are natural enemies of several herbivorous insect pest species. During oviposition they inject a mixture of factors, which include venom, into the host, ensuring the successful parasitism and the development of their progeny. Although these parasitoid factors are known to be responsible for host manipulation, such as immune system suppression, little is known...

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Dosimetric experience with 2 commercially available multilumen balloon-based brachytherapy to deliver accelerated partial-breast irradiation

Weihua Fu, Jong Oh Kim, Alex S.J. Chen, Kiran Mehta, more

Medical Dosimetry > 2015 > 40 > 3 > 195-200

The purpose of this work was to report dosimetric experience with 2 kinds of multilumen balloon (MLB), 5-lumen Contura MLB (C-MLB) and 4-lumen MammoSite MLB (MS-MLB), to deliver accelerated partial-breast irradiation, and compare the ability to achieve target coverage and control skin and rib doses between 2 groups of patients treated with C-MLB and MS-MLB brachytherapy. C-MLB has 5 lumens, the 4...

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A new anti-infective strategy to reduce the spreading of antibiotic resistance by the action on adhesion-mediated virulence factors in Staphylococcus aureus

Rosanna Papa, Marco Artini, Andrea Cellini, Marco Tilotta, more

Microbial Pathogenesis > 2013 > 63 > Complete > 44-53

Staphylococcus aureus is a flexible microbial pathogen frequently isolated from community-acquired and nosocomial infections. S. aureus expresses a wide array of secreted and cell surface-associated virulence factors, including proteins that promote adhesion to damaged tissue and to the surface of host cells, and that bind proteins in blood to help evade immune responses. Furthermore, surface proteins...

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Molecular and Functional Analysis of the Large 5′ Promoter Region of CFTR Gene Revealed Pathogenic Mutations in CF and CFTR-Related Disorders

Sonia Giordano, Felice Amato, Ausilia Elce, Maria Monti, more

The Journal of Molecular Diagnostics > 2013 > 15 > 3 > 331-340

Patients with cystic fibrosis (CF) manifest a multisystemic disease due to mutations in the gene encoding the cystic fibrosis transmembrane conductance regulator (CFTR); despite extensive testing of coding regions, a proportion of CF alleles remains unidentified. We studied 118 patients with CF and CFTR-related disorders, most with one or both unknown mutations after the scanning of CFTR coding regions,...

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A simple and reliable methodology to detect egg white in art samples

Michela Gambino, Francesca Cappitelli, Cristina Cattò, Aristodemo Carpen, more

Journal of Biosciences > 2013 > 38 > 2 > 397-408

A protocol for a simple and reliable dot-blot immunoassay was developed and optimized to test work of art samples for the presence of specific proteinaceus material (i.e. ovalbumin-based). The analytical protocol has been extensively set up with respect, among the other, to protein extraction conditions, to densitometric analysis and to the colorimetric reaction conditions. Feasibility evaluation...

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Deamidation at Asparagine and Glutamine As a Major Modification upon Deterioration/Aging of Proteinaceous Binders in Mural Paintings

Gabriella Leo, Ilaria Bonaduce, Alessia Andreotti, Gennaro Marino, more

Analytical Chemistry > 2011 > 83 > 6 > 2056 - 2064

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Stoichiometry and Topology of the Complex of the Endogenous ATP Synthase Inhibitor Protein IF ₁ with Calmodulin

Daniela Pagnozzi, Leila Birolo, Gabriella Leo, Stefania Contessi, more

Biochemistry > 2010 > 49 > 35 > 7542 - 7552

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The co-chaperone BAG3 interacts with the cytosolic chaperonin CCT: New hints for actin folding

Bianca Fontanella, Leila Birolo, Giuseppe Infusini, Claudia Cirulli, more

International Journal of Biochemistry and Cell Biology > 2010 > 42 > 5 > 641-650

It has been recently hypothesized that BAG3 protein, a co-chaperone of Hsp70/Hsc70, is involved in the regulation of several cell processes, such as apoptosis, autophagy and cell motility. Following the identification of Hsc70/Hsp70, further BAG3 molecular partners such as PLC-γ and HspB8 were likewise identified, thus contributing to the characterization of the mechanisms and the biological roles...

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hnRNP H1 and intronic G runs in the splicing control of the human rpL3 gene

Annapina Russo, Gabriella Siciliano, Morena Catillo, Chiara Giangrande, more

BBA - Gene Regulatory Mechanisms > 2010 > 1799 > 5-6 > 419-428

By generating mRNA containing a premature termination codon (PTC), alternative splicing (AS) can quantitatively regulate the expression of genes that are degraded by nonsense-mediated mRNA decay (NMD). We previously demonstrated that AS-induced retention of part of intron 3 of rpL3 pre-mRNA produces an mRNA isoform that contains a PTC and is targeted for decay by NMD. We also demonstrated that overexpression...

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cis-acting sequences and trans-acting factors in the localization of mRNA for mitochondrial ribosomal proteins

Annapina Russo, Claudia Cirulli, Angela Amoresano, Pietro Pucci, more

BBA - Gene Regulatory Mechanisms > 2008 > 1779 > 12 > 820-829

mRNA localization is a conserved post-transcriptional process crucial for a variety of systems. Although several mechanisms have been identified, emerging evidence suggests that most transcripts reach the protein functional site by moving along cytoskeleton elements. We demonstrated previously that mRNA for mitochondrial ribosomal proteins are asymmetrically distributed in the cytoplasm, and that...

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Protease treatment affects both invasion ability and biofilm formation in Listeria monocytogenes

Catia Longhi, Gian Luca Scoarughi, Federica Poggiali, Andrea Cellini, more

Microbial Pathogenesis > 2008 > 45 > 1 > 45-52

Listeria monocytogenes is a notably invasive bacterium associated with life-threatening food-borne disease in humans. Several surface proteins have been shown to be essential in the adhesion of L. monocytogenes, and in the subsequent invasion of phagocytes. Because the control of the invasion of host cells by Listeria could potentially hinder its spread in the infected host, we have examined the effects...

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Peptidoglycan and Muropeptides from Pathogens Agrobacterium and Xanthomonas Elicit Plant Innate Immunity: Structure and Activity

Gitte Erbs, Alba Silipo, Shazia Aslam, Cristina De Castro, more

Chemistry & Biology > 2008 > 15 > 5 > 438-448

Peptidoglycan (PGN) is a unique and essential structural part of the bacterial cell wall. PGNs from two contrasting Gram-negative plant pathogenic bacteria elicited components characteristic of the innate immune system in Arabidopsis thaliana, such as transcription of the defense gene PR1, oxidative burst, medium alkalinization, and formation of callose. Highly purified muropeptides from PGNs were...

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Tubulin nitration in human gliomas

Gabriella Fiore, Carlo Di Cristo, Gianluca Monti, Angela Amoresano, more

Neuroscience Letters > 2006 > 394 > 1 > 57-62

Immunohistochemical and biochemical investigations showed that significant protein nitration occurs in human gliomas, especially in grade IV glioblastomas at the level of astrocytes and oligodendrocytes and neurones. Enhanced alpha-tubulin immunoreactivity was co-present in the same elements in the glioblastomas. Proteomic methodologies were employed to identify a nitrated protein band at 55kDa as...