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Protein labeling with synthetic fluorescent probes is a key technology in chemical biology and biomedical research. A sensitive and efficient modular labeling approach (SLAP) was developed on the basis of a synthetic small‐molecule recognition unit (Ni‐trisNTA) and the genetically encoded minimal protein His6‐10‐tag. High‐density protein tracing by SLAP was demonstrated. This technique allows super‐resolution...
Ein “Small Labeling Pair” (SLAP) für die Einzelmolekülbildgebung wird von M. Heilemann, R. Tampé et al. in ihrer Zuschrift auf S. 10354 ff. vorgestellt. Die niedermolekulare Erkennungseinheit (Ni‐trisNTA) und eine genetisch kodierte minimale His6‐10‐Markierung ermöglichen die schnelle, ortsspezifische und stöchiometrische Markierung mit hoher Dichte. Die enge Nachbarschaft, die mit SLAP erreicht wird,...
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