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Small chemical/biological interaction pairs are at the forefront in tracing protein function and interaction at high signal‐to‐background ratios in cellular pathways. However, the optimal design of scaffold, linker, and chelator head still deserve systematic investigation to achieve the highest affinity and kinetic stability for in vitro and especially cellular applications. We report on a library...
TrisNTA‐Chelatoren bestehen aus drei metallkomplexierenden N‐Nitrilotriessigsäure(NTA)‐Derivaten, die über eine Gerüststruktur verbunden sind. Gekoppelt an einen Fluorophor oder einen anderen Reporter können trisNTAs als Sonden für die Kennzeichnung histidinmarkierter Proteine in lebenden Zellen dienen. R. Tampé et al. berichten in ihrer Zuschrift auf S. 12575 über große Affinitäts‐ und Stabilitätsunterschiede,...
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