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On-chip optical detection of chemiluminescent reactions is presented. The device is based on the integration of thin film hydrogenated amorphous silicon photosensors on a functionalized glass substrate ensuring both a good optical coupling and an optimal separation between biological or chemical reagents and the sensing elements. The sensor has been characterized and optimized using the chemiluminescent...
A diagnostic device suitable for Parvovirus B19 genotyping and quantitative DNA detection was developed, based on hybridization with immobilized oligonucleotide probes and chemiluminescence detection. The microfluidic chamber was directly coupled with a CCD sensor in a “contact” imaging configuration to obtain a portable device suitable for point-of-care applications.
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