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Proteins of the large ribosomal subunit of rat liver (TP 60) were immobilized by diffusion transfer onto nitrocellulose after two-dimensional polyacrylamide gel electrophoresis (2-D PAGE). Incubation of the TP 60 blots with 32 P-labeled 5 S RNA under defined ionic conditions (300 mM KCl, 20 mM MgCl 2 ) resulted in specific binding to a limited set of ribosomal proteins consisting...
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