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Bacillus cereus sphingomyelinase activity was assayed on large unilamellar vesicles composed of sphingomyelin (SM)/cholesterol (Ch) mixtures at varying proportions. Natural (egg) SM was used with a gel-fluid transition temperature at ca. 40 o C. When the enzyme was assayed at 37 o C, the activity on pure SM was exceedingly low, but a small increase was observed as soon as some Ch was...
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