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Über Zwei‐Photonen‐Absorption können Rutheniumkomplexe, die an die Kern‐DNA fixierter oder lebender Zellen binden, im optischen Fenster biologischer Proben angeregt werden. In ihrer Zuschrift auf S. 3435 ff. zeigen J. A. Thomas und Mitarbeiter, dass die langlebige 3MLCT‐Emission diese “Lichtschalter”‐Komplexe (>100 ns) auch als Sonden für die bildgebende Phosphoreszenzlebensdauer‐Mikroskopie geeignet...
The first transition‐metal complex‐based two‐photon absorbing luminescence lifetime probes for cellular DNA are presented. This allows cell imaging of DNA free from endogenous fluorophores and potentially facilitates deep tissue imaging. In this initial study, ruthenium(II) luminophores are used as phosphorescent lifetime imaging microscopy (PLIM) probes for nuclear DNA in both live and fixed cells...
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