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The possible breakdown of chlorophyll by irradiation was investigated. Chlorophyll b standard (3 ppm) was added to a methanol solution containing 1% linoleic acid. Irradiation up to 20 kGy was performed with or without N2-bubbling, and nonirradiated control was also prepared. Residual chlorophyll b content was analyzed by HPLC and UV-visible spectra after irradiation, and color was measured during...
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