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Fast dissolution nanofiber membranes were prepared using electrospinning process with Ferulic Acid (FA)--a traditional Chinese medicine as the active pharmaceutical ingredients and a pharmaceutical hydrophilic polymer--polyvinylpyrrolidone K30 as the filament-forming matrix. Field Emission Scanning Electronic Microscopy (FE-SEM), Differential Scanning Calorimetry (DSC), Attenuated Total Reflection...
Novel membranes were prepared through covalently coupling of chitosan (CS) to activate nylon membrane after the reaction of the microporous nylon membrane with formaldehyde. Non-specific adsorption on the CS-coated nylon membrane decreased greatly. Yellow 3G-P/Reactive Red 120 were then covalently immobilized onto composite membrane as dye ligands. These Red 120/Yellow 3G-P-CS-membranes were used...
The behavior of bromelain adsorption on the dye- ligand affinity membrane was investigated at 277,298 and 310 K, which was chitosan (CS) coated to decrease the non-specific adsorption of protein. Reactive Blue 4 was immobilized onto the CS-coated nylon membrane as an affinity ligand. In order to examine the rate limiting step of Reactive Blue 4-immobilized composite membrane adsorption, the two adsorption...
Electrospun nylon-6 nanofibrous membrane with fiber diameters in the range of 50-200 nm were prepared and employed as affinity membrane material for papain adsorption due to their excellent chemical and thermal resistance as well as high wettability. In this work, covalent coupling of chitosan (CS) to activated nylon membrane was performed after the reaction of the nanofibrous nylon membrane with...
Nylon membranes are first hydrolyed with dilute HCl and then treated with chitosan before being used as the affinity carrier. Papain as a ligand has been immobilized on the activated membranes with glutaraldehyde as a crosslinking agent. The factors involving with the activity of immobilized papain, such as concentration of glutaraldehyde, pH, temperature, reaction time, and the amount of added papain...
It is commonly recognized that damage to bone tissue begins to occur in response to these supra-physiologic mechanical loading. Parathyroid hormone (PTH) plays a key role on the anabolic effects of osteoblast. However, the effects of N-terminal 1-34 fragment of human PTH (hPTHl-34) on osteoblast under supra-threshold mechanical loading are unknown. We recently used a stretch-induced from the hypotonic...
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