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Ventral view of a 3 day‐old zebrafish in which lateral plate mesoderm‐derivatives including the heart were lineage‐labeled (magenta) using transgenes for drl:creERT2 and the loxP‐based reporter ubi:Switch (brightfield and mCherry fluorescence composite, 20x lens, Zeiss LSM 880 confocal). From: Heterogeneity and genomic loci of ubiquitous transgenic Cre reporter lines in zebrafish; Lalonde RL Kemmler...
Background
The most‐common strategy for zebrafish Cre/lox‐mediated lineage labeling experiments combines ubiquitously expressed, lox‐based Switch reporter transgenes with tissue‐specific Cre or 4‐OH‐Tamoxifen‐inducible CreERT2 driver lines. Although numerous Cre driver lines have been produced, only a few broadly expressed Switch reporters exist in zebrafish and their generation by random transgene...
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