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Background Genome scale metabolic reconstructions are developed to efficiently engineer biocatalysts and bioprocesses based on a rational approach. However, in most reconstructions, due to the lack of appropriate measurements, experimentally determined growth parameters are simply taken from literature including other organisms, which reduces the usefulness and suitability of these models. Pseudomonas putida...
In this study, we replaced the natural start codons of different enzymes in the central carbon metabolism of Corynebacterium glutamicum to influence their activity toward improved production of the feed amino acid lysine. It was found that the translational start codon directly affects the intracellular activity of the encoded enzyme, whereby the common ATG generally led to higher values as compared...
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