Search results for: Danuta Siluk

Items from 1 to 20 out of 21 results

article

Determination of tocopherols and tocotrienols in human breast adipose tissue with the use of high performance liquid chromatography–fluorescence detection

Ewa Bartosińska, Julia Jacyna, Agnieszka Borsuk-De Moor, Michał Kaliszan, more

Biomedical Chromatography > 32 > 12 > n/a - n/a

Tocopherols and tocotrienols have been extensively studied owing to their anticancer potential, especially against breast cancer. Therefore, the aim of this study was to quantitatively determine tocochromanols in human breast adipose tissue with the use of HPLC–FLD. The sample preparation procedure included homogenization and solvent extraction with isopropanol–ethanol–0.1% formic acid mixture prior...

article

Ionization of tocopherols and tocotrienols in atmospheric pressure chemical ionization

Ewa Bartosińska, Agnieszka Borsuk‐De Moor, Danuta Siluk, Michał J. Markuszewski, more

Rapid Communications in Mass Spectrometry > 32 > 11 > 919 - 927

Rationale Tocopherols and tocotrienols are chemical compounds insusceptible to the ionization process under atmospheric pressure conditions. Therefore, the selection of the optimal ion source settings for their quantification requires special attention. The aim of this study was to analyse the influence of the APCI source parameters on the response of tocochromanols and two related compounds. Methods...

article

Quantitative determination of trigonelline in mouse serum by means of hydrophilic interaction liquid chromatography–MS/MS analysis: Application to a pharmacokinetic study

Damian Szczesny, Ewa Bartosińska, Julia Jacyna, Małgorzata Patejko, more

Biomedical Chromatography > 32 > 2 > n/a - n/a

Trigonelline is a pyridine alkaloid found in fenugreek seeds and coffee beans. Most of the previous studies are concerned with the quantification of trigonelline along with other constituents in coffee herbs or beverages. Only a few have focused on its determination in animal or human tissues by applying different modes of HPLC with UV or MS detection. The aim of the study was to develop and validate...

article

HPLC-APCI-MS/MS method development and validation for determination of tocotrienols in human breast adipose tissue

Ewa Bartosińska, Julia Jacyna, Agnieszka Borsuk-De Moor, Michał Kaliszan, more

Talanta > 2018 > 176 > C > 108-115

For the last decade, significant attention has been paid to the potential role of tocotrienols in prevention and therapy of breast cancer. Therefore, the aim of this study was to develop and validate analytical method for quantitative determination of tocotrienols (α-, β-, γ- and δ-tocotrienol) in human breast adipose tissue with the use of high performance liquid chromatography coupled with APCI-MS/MS...

article

“Molecularly imprinted chromatography” fails to distinguish homeopathic remedy from placebo

Roman Kaliszan, Małgorzata Waszczuk‐Jankowska, Danuta Siluk, Bartosz Wielgomas

Journal of Separation Science > 40 > 20 > 3976 - 3976

article

The pharmacokinetics of dexmedetomidine during long-term infusion in critically ill pediatric patients. A Bayesian approach with informative priors

Paweł Wiczling, Alicja Bartkowska-Śniatkowska, Oliwia Szerkus, Danuta Siluk, more

Journal of Pharmacokinetics and Pharmacodynamics > 2016 > 43 > 3 > 315-324

The purpose of this study was to assess the pharmacokinetics of dexmedetomidine in the ICU settings during the prolonged infusion and to compare it with the existing literature data using the Bayesian population modeling with literature-based informative priors. Thirty-eight patients were included in the analysis with concentration measurements obtained at two occasions: first from 0 to 24 h after...

article

GC/MS technique and AMDIS software application in identification of hydrophobic compounds of grasshoppers’ abdominal secretion (Chorthippus spp.)

Magdalena Buszewska-Forajta, Renata Bujak, Arlette Yumba-Mpanga, Danuta Siluk, more

Journal of Pharmaceutical and Biomedical Analysis > 2015 > 102 > Complete > 331-339

The automated mass spectral deconvolution and identification system (AMDIS) is a modern analytical tool, mostly used as a data processing method in environmental studies. The most attractive feature of that software is a fast, automatic data processing, which includes removal of interferences from the overlapping peaks and purification of the obtained mass spectra. The identification of analytes is...

article

Development and validation of UHPLC method for the determination of cyclosporine A in biological samples

Oliwia Szerkus, Eliza Wolska, Wiktoria Struck‐Lewicka, Danuta Siluk, more

Biomedical Chromatography > 28 > 6 > 802 - 809

The aim of the study was to develop and validate a simple and rapid method for the determination of cyclosporine A (CsA) in ocular rabbit tissues using reversed‐phase ultra‐high‐performance liquid chromatography (UHPLC) with UV detection. Previous publications on chromatographic methods of CsA determination in ocular tissues involved only reversed‐phase HPLC separation, usually in combination with...

article

Identification of lipid fraction constituents from grasshopper (Chorthippus spp.) abdominal secretion with potential activity in wound healing with the use of GC–MS/MS technique

Magdalena Buszewska-Forajta, Danuta Siluk, Wiktoria Struck-Lewicka, Joanna Raczak-Gutknecht, more

Journal of Pharmaceutical and Biomedical Analysis > 2014 > 89 > C > 56-66

In recent years biologically active compounds isolated from insects call special interest of drug researchers. According to some Polish etnopharmacological observations, secretion from the grasshopper's abdomen (Orthoptera family) is believed to speed up the process of wound healing.In the present work we focused on determination of main components of the lipid fraction of material from grasshopper...

article

Determination of Water-Soluble Components of Abdominal Secretion of Grasshopper (Chorthippus spp.) by GC/MS/MS in Search for Potential Wound Healing Agents

Magdalena Buszewska-Forajta, Wiktoria Struck-Lewicka, Renata Bujak, Danuta Siluk, more

Chromatographia > 2014 > 77 > 15-16 > 1091-1102

Wound healing is still a serious medical problem due to process complexity and lack of effective medicaments. This is particularly true in the treatment of wounds arising in the course of such diseases as AIDS or diabetes. Therefore, scientific efforts are focused on the search for new compounds of natural origin, which could be used as medicines or evaluated for subsequent drug design. In folk medicine,...

article

Liquid chromatography tandem mass spectrometry study of urinary nucleosides as potential cancer markers

Wiktoria Struck, Danuta Siluk, Arlette Yumba-Mpanga, Marcin Markuszewski, more

Journal of Chromatography A > 2013 > 1283 > Complete > 122-131

The aim of this work was a comprehensive analysis of metabolite profiles of nucleosides from biological samples obtained from patients with urogenital cancer disease as well as an interpretation of cancer-related patterns of the analyzed profiles. In our study we proposed a targeted approach that was focused on the simultaneous determination of twelve nucleosides from over a hundred urine samples...

article

19F MRI of 3D CEM cells to study the effects of tocopherols and tocotrienols

Dorota Bartusik, Boguslaw Tomanek, Danuta Siluk, Roman Kaliszan

Journal of Pharmaceutical and Biomedical Analysis > 2010 > 53 > 3 > 599-602

Oximetry of the human T-Lymphoblastoid (CEM) cells was measured using ¹⁹ F magnetic resonance imaging ( ¹⁹ F MRI). The cells were treated with the analogues of vitamin E, α-, γ-, δ-tocopherols and corresponding tocotrienols, ex vivo in three-dimensional (3D) cell culture. The study showed that ¹⁹ F MRI allows to measure the effect of the analogues due to changes of oxygenation,...

article

Quantitative determination of fenoterol and fenoterol derivatives in rat plasma using on-line immunoextraction and liquid chromatography/mass spectrometry

Hee Seung Kim, Danuta Siluk, Irving W. Wainer

Journal of Chromatography A > 2009 > 1216 > 16 > 3526-3532

An on-line immunoextraction and liquid chromatography/mass spectrometry (LC/MS) method was developed and validated for the determination of R,R′-fenoterol, R,R′-methoxyfenoterol and R,S′-naphthylfenoterol in rat plasma. Sample preparation involved immunoextraction of analytes using an antibody raised against R,R′- and R,S′-aminofenoterol that was immobilized onto chromatographic support. LC was performed...

article

The application of 19F magnetic resonance ex vivo imaging of three-dimensional cultured breast cancer cells to study the effect of δ-tocopherol

Dorota Bartusik, Boguslaw Tomanek, Danuta Siluk, Roman Kaliszan, more

Analytical Biochemistry > 2009 > 387 > 2 > 315-317

The aim of this study was to determine the role of δ-tocopherol in breast cancer cell growth ex vivo. Human gland mammary adenocarcinoma (MCF-7) and human T-lymphoblastoid (CEM) cells were cultured in the presence of δ-tocopherol at various concentrations (0–750μM) for 5days. We have grown 3D ex vivo breast cancer cell cultures in the hollow fiber bioreactor (HFBR). ¹⁹ F magnetic resonance...

article

HPLC–electrospray mass spectrometric assay for the determination of (R,R)-fenoterol in rat plasma

Danuta Siluk, Hee Seung Kim, Tyler Cole, Irving W. Wainer

Journal of Pharmaceutical and Biomedical Analysis > 2008 > 48 > 3 > 960-964

A fast and specific liquid chromatography–mass spectrometry method for the determination of (R,R)-fenoterol ((R,R)-Fen) in rat plasma has been developed and validated. (R,R)-Fen was extracted from 125μl of plasma using solid phase extraction and analyzed on Atlantis HILIC Silica 3μm column. The mobile phase was composed of acetonitrile:ammonium acetate (pH 4.1; 20mM) (85:15, v/v), at a flow rate of...

article

HPLC–atmospheric pressure chemical ionization mass spectrometric method for enantioselective determination of R,S-propranolol and R,S-hyoscyamine in human plasma

Danuta Siluk, Donald E. Mager, Naomi Gronich, Darrell Abernethy, more

Journal of Chromatography B > 2007 > 859 > 2 > 213-221

A method for the simultaneous determination of R- and S-propranolol and R- and S-hyoscyamine in human plasma was developed, validated and applied to the analysis of samples from a clinical study. Sample preparation was performed by solid-phase extraction of 2ml of human plasma using Oasis MCX cartridges and the enantioselective separations were achieved using a Chirobiotic V chiral stationary phase...

article

Enantioselective liquid chromatography–mass spectrometry assay for the determination of ifosfamide and identification of the N-dechloroethylated metabolites of ifosfamide in human plasma

Regina V. Oliveira, Joelle M. Onorato, Danuta Siluk, Christine M. Walko, more

Journal of Pharmaceutical and Biomedical Analysis > 2007 > 45 > 2 > 295-303

A sensitive and specific liquid chromatography–mass spectrometry (LC–MS) method has been developed and validated for the enantioselective determination of ifosfamide [(R)-IF and (S)-IF] in human plasma and for the detection of the N-dechloroethylated metabolites of IF, 2-N-dechloroethylifosfamide [(R)-2-DCl-IF and (S)-2-DCl-IF] and 3-N-dechloroethylifosfamide [(R)-3-DCl-IF and (S)-3-DCl-IF]. IF, 2-DCl-IF...

article

A validated liquid chromatography method for the simultaneous determination of vitamins A and E in human plasma

Danuta Siluk, Regina V. Oliveira, Maria Esther-Rodriguez-Rosas, Shari Ling, more

Journal of Pharmaceutical and Biomedical Analysis > 2007 > 44 > 4 > 1001-1007

A fast (15min) and simple HPLC method for determination of all-trans-retinol and α-, γ- and δ-tocopherols in human plasma has been developed. The assay utilized 200μl of plasma to which 20μl of internal standard solution (retinol acetate) was added followed by 200μl of water, 400μl of ethanol and 800μl of hexane. The hexane layer was collected, evaporated, the residue dissolved in 200μl of methanol...

article

Simultaneous determination of buprenorphine, norbuprenorphine and the enantiomers of methadone and its metabolite (EDDP) in human plasma by liquid chromatography/mass spectrometry

Maria Esther Rodriguez-Rosas, Michelle R. Lofwall, Eric C. Strain, Danuta Siluk, more

Journal of Chromatography B > 2007 > 850 > 1-2 > 538-543

A previously reported enantioselective LC–MS assay for the determination of (R)- and (S)-methadone [Met] and (R)- and (S)-2-ethylidene-1,5-dimethyl-3,3-diphenyl-pyrrolidine [EDDP] (the primary metabolite of Met) has been adapted for use in the simultaneous determination of the plasma concentrations of Met, EDDP, buprenorphine (Bu) and norbuprenorphine (norBu). All of the target compounds were separated...

article

Human red blood cells targeted metabolome analysis of glycolysis cycle metabolites by capillary electrophoresis using an indirect photometric detection method

Michał J. Markuszewski, Marta Szczykowska, Danuta Siluk, Roman Kaliszan

Journal of Pharmaceutical and Biomedical Analysis > 2005 > 39 > 3-4 > 636-642

A capillary electrophoretic (CE) method with UV indirect spectrophotometric detection for determination of the main compounds of glycolysis in human erythrocytes has been elaborated. Blood samples for electrophoretic analysis were prepared by isolation of erythrocytes, lysis of the cells by heating in double-distilled water and subsequent ultrafiltration with a centrifuge equipped with filter devices:...

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Search results for: Danuta Siluk

Determination of tocopherols and tocotrienols in human breast adipose tissue with the use of high performance liquid chromatography–fluorescence detection

Ionization of tocopherols and tocotrienols in atmospheric pressure chemical ionization

Quantitative determination of trigonelline in mouse serum by means of hydrophilic interaction liquid chromatography–MS/MS analysis: Application to a pharmacokinetic study

HPLC-APCI-MS/MS method development and validation for determination of tocotrienols in human breast adipose tissue

“Molecularly imprinted chromatography” fails to distinguish homeopathic remedy from placebo

The pharmacokinetics of dexmedetomidine during long-term infusion in critically ill pediatric patients. A Bayesian approach with informative priors

GC/MS technique and AMDIS software application in identification of hydrophobic compounds of grasshoppers’ abdominal secretion (Chorthippus spp.)

Development and validation of UHPLC method for the determination of cyclosporine A in biological samples

Identification of lipid fraction constituents from grasshopper (Chorthippus spp.) abdominal secretion with potential activity in wound healing with the use of GC–MS/MS technique

Determination of Water-Soluble Components of Abdominal Secretion of Grasshopper (Chorthippus spp.) by GC/MS/MS in Search for Potential Wound Healing Agents

Liquid chromatography tandem mass spectrometry study of urinary nucleosides as potential cancer markers

19F MRI of 3D CEM cells to study the effects of tocopherols and tocotrienols

Quantitative determination of fenoterol and fenoterol derivatives in rat plasma using on-line immunoextraction and liquid chromatography/mass spectrometry

The application of 19F magnetic resonance ex vivo imaging of three-dimensional cultured breast cancer cells to study the effect of δ-tocopherol

HPLC–electrospray mass spectrometric assay for the determination of (R,R)-fenoterol in rat plasma

HPLC–atmospheric pressure chemical ionization mass spectrometric method for enantioselective determination of R,S-propranolol and R,S-hyoscyamine in human plasma

Enantioselective liquid chromatography–mass spectrometry assay for the determination of ifosfamide and identification of the N-dechloroethylated metabolites of ifosfamide in human plasma

A validated liquid chromatography method for the simultaneous determination of vitamins A and E in human plasma

Simultaneous determination of buprenorphine, norbuprenorphine and the enantiomers of methadone and its metabolite (EDDP) in human plasma by liquid chromatography/mass spectrometry

Human red blood cells targeted metabolome analysis of glycolysis cycle metabolites by capillary electrophoresis using an indirect photometric detection method

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