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Limulus Factor C is a potent antagonist of endotoxin from Gram-negative bacteria. A fusion construct containing full length Factor C has been cloned intoSpodoptera frugiperda Sf9 cell. Stable Sf9 cell transfectants were obtained using Zeocin selection for 2 weeks. The recombinant Factor C (rFC) was secreted into the culture medium at 9 mg l−1. Both the crude and partially purified rFC were able to...
The cDNA encoding Carcinoscorpius rotundicauda Factor C, CrFC, when expressed in mammalian cells, produced full length and truncated variants of rFC. In an in vitro coupled transcription-translation system, aberrant products were generated from pCDNAI/CrFC21, as opposed to pCIneo/CrFC21 which gave rise to the expected polypeptide. COS-1 cells transfected with pCIneo/CrFC21 expressed Factor C-related...
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