Search results for: Graciela L. Salerno

Items from 1 to 20 out of 36 results

article

Target of rapamycin signaling is tightly and differently regulated in the plant response under distinct abiotic stresses

Cintia M. Pereyra, Néstor R. Aznar, Marianela S. Rodriguez, Graciela L. Salerno, more

Planta > 2020 > 251 > 1 > 1-14

Main conclusion TOR signaling is finely regulated under diverse abiotic stresses and may be required for the plant response with a different time-course depending on the duration and nature of the stress. Abstract Target of rapamycin (TOR) signaling is a central regulator of growth and development in eukaryotic organisms. However, its regulation under stress conditions has not yet been elucidated...

article

Raphidiopsis mediterranea (Nostocales) exhibits a flexible growth strategy under light and nutrient fluctuations in contrast to Planktothrix agardhii (Oscillatoriales)

Anabella Aguilera, Luis Aubriot, Ricardo O. Echenique, Jorge L. Donadelli, more

Hydrobiologia > 2019 > 839 > 1 > 145-157

Raphidiopsis mediterranea is a freshwater cyanobacterium that forms toxic blooms in eutrophic water bodies. Factors controlling its proliferation have not been explored in detail. We investigated R. mediterranea autecology by (i) analyzing its dynamics in a hypertrophic shallow lake dominated by Planktothrix agardhii (Oscillatoriales) and its relationship with environmental factors; and (ii) studying...

article

Ancient signal for nitrogen status sensing in the green lineage: Functional evidence of CDPK repertoire in Ostreococcus tauri

Gonzalo Caló, Dana Scheidegger, Giselle M.A. Martínez-Noël, Graciela L. Salerno

Plant Physiology and Biochemistry > 2017 > 118 > C > 377-384

Calcium-dependent protein kinases (CDPKs) regulate plant development and many stress signalling pathways through the complex cytosolic [Ca²⁺] signalling. The genome of Ostreococcus tauri (Ot), a model prasinophyte organism that is on the base of the green lineage, harbours three sequences homologous to those encoding plant CDPKs with the three characteristic conserved domains (protein kinase, autoregulatory/autoinhibitory,...

article

Synergistic effects of nutrients and light favor Nostocales over non-heterocystous cyanobacteria

Anabella Aguilera, Luis Aubriot, Ricardo O. Echenique, Graciela L. Salerno, more

Hydrobiologia > 2017 > 794 > 1 > 241-255

Blooms of Nostocales (Cyanobacteria) are thought to be invading subtropical and temperate water bodies. According to nutrient stoichiometry and physiological differences between cyanobacterial groups, the replacement of non-heterocystous species by Nostocales is favored when dissolved inorganic nitrogen decreases. However, some studies have shown different trends. We used laboratory bioassays to evaluate...

article

Sucrose in bloom‐forming cyanobacteria: loss and gain of genes involved in its biosynthesis

María A. Kolman, Graciela L. Salerno

Environmental Microbiology > 18 > 2 > 439 - 449

Bloom‐forming cyanobacteria are widely distributed in freshwater ecosystems. To cope with salinity fluctuations, cyanobacteria synthesize compatible solutes, such as sucrose, to maintain the intracellular osmotic balance. The screening of cyanobacterial genomes revealed that homologues to sucrose metabolism‐related genes only occur in few bloom‐forming strains, mostly belonging to Nostocales and Stigonematales...

article

Functional characterization of Synechococcus amylosucrase and fructokinase encoding genes discovers two novel actors on the stage of cyanobacterial sucrose metabolism

Macarena Perez-Cenci, Graciela L. Salerno

Plant Science > 2014 > 224 > C > 95-102

Plants and most cyanobacteria metabolize sucrose (Suc) with a similar set of enzymes. In Synechococcus sp. PCC 7002, a marine cyanobacterium strain, genes involved in Suc synthesis (spsA and sppA) have been characterized; however, its breakdown was still unknown. Indeed, neither invertase nor sucrose synthase genes, usually found in plants and cyanobacteria, were found in that Synechococcus genome...

article

First evidence of sucrose biosynthesis by single cyanobacterial bimodular proteins

Giselle M.A. Martínez-Noël, Andrea C. Cumino, María de los Angeles Kolman, Graciela L. Salerno

FEBS Letters > 2013 > 587 > 11 > 1669-1674

The net synthesis of sucrose (Suc) is catalysed by the sequential action of Suc-phosphate synthase (SPS) and Suc-phosphate phosphatase (SPP). SPS and SPP from Anabaena sp. PCC 7120 (7120-SPS and 7120-SPP) define minimal catalytic units. Bidomainal SPSs, where both units are fused, occur in plants and cyanobacteria, but they display only SPS activity. Using recombinant proteins that have fused 7120-SPS...

article

The unique nucleotide specificity of the sucrose synthase from Thermosynechococcus elongatus

Carlos M. Figueroa, Matías D. Asención Diez, Misty L. Kuhn, Sheila McEwen, more

FEBS Letters > 2013 > 587 > 2 > 165-169

Sucrose synthase catalyzes the reversible conversion of sucrose and UDP into fructose and UDP-glucose. In filamentous cyanobacteria, the sucrose cleavage direction plays a key physiological function in carbon metabolism, nitrogen fixation, and stress tolerance. In unicellular strains, the function of sucrose synthase has not been elucidated. We report a detailed biochemical characterization of sucrose...

article

A mitochondrial alkaline/neutral invertase isoform (A/N-InvC) functions in developmental energy-demanding processes in Arabidopsis

Mariana L. Martín, Leandra Lechner, Eduardo J. Zabaleta, Graciela L. Salerno

Planta > 2013 > 237 > 3 > 813-822

Recent findings demonstrate that alkaline/neutral invertases (A/N-Invs), enzymes that catalyze the breakdown of sucrose into glucose and fructose, are essential proteins in plant life. The fact that different isoforms are present in multiple locations makes them candidates for the coordination of metabolic processes. In the present study, we functionally characterized the encoding gene of a novel...

article

Sucrose synthase in unicellular cyanobacteria and its relationship with salt and hypoxic stress

María A. Kolman, Leticia L. Torres, Mariana L. Martin, Graciela L. Salerno

Planta > 2012 > 235 > 5 > 955-964

Higher plants and cyanobacteria metabolize sucrose (Suc) by a similar set of enzymes. Suc synthase (SuS, A/UDP-glucose: d-fructose 2-α-d-glucosyl transferase) catalyzes a reversible reaction. However, it is in the cleavage of Suc that this enzyme plays an important role in vivo, providing sugar nucleotides for polysaccharide biosynthesis. In cyanobacteria, SuS occurrence has been reported in heterocyst-forming...

article

Genetic Diversity of cry Gene Sequences of Bacillus thuringiensis Strains Analyzed by Denaturing Gradient Gel Electrophoresis

María E. Vidal-Domínguez, Macarena Perez-Cenci, Graciela L. Salerno, Corina M. Berón

Current Microbiology > 2011 > 62 > 3 > 866-870

PCR has been widely used to identify cry-type genes, to determine their distribution, to detect new such genes and to predict insecticidal activities. We describe here a molecular approach to analyze the genetic diversity of B. thuringiensis cry-like genes based on denaturing gradient gel electrophoresis (DGGE). This analysis demonstrated that different B. thuringiensis isolates can be distinguished...

article

Differential roles of alkaline/neutral invertases in Nostoc sp. PCC 7120: Inv-B isoform is essential for diazotrophic growth

Walter A. Vargas, Carolina N. Nishi, Laura E. Giarrocco, Graciela L. Salerno

Planta > 2011 > 233 > 1 > 153-162

The presence of two alkaline/neutral invertases (Inv-A and Inv-B) in the filaments of Nostoc (also named Anabaena) sp. strain PCC 7120 and the involvement of sucrose metabolism in nitrogen fixation led us to investigate the physiological function of those isoforms in cells growing under different nitrogen sources. The highest expression level of each encoding gene was obtained in the presence of ammonium...

article

The proteins involved in sucrose synthesis in the marine cyanobacterium Synechococcus sp. PCC 7002 are encoded by two genes transcribed from a gene cluster

Andrea C. Cumino, Macarena Perez-Cenci, Laura E. Giarrocco, Graciela L. Salerno

FEBS Letters > 2010 > 584 > 22 > 4655-4660

It has been reported that higher plants and cyanobacteria synthesize sucrose (Suc) by a similar sequential action of sucrose-phosphate synthase (SPS) and sucrose-phosphate phosphatase (SPP). In the genome of the marine unicellular cyanobacterium Synechococcus sp. PCC 7002 there is a sequence that was not annotated as a putative SPP encoding gene (sppA), although the sequence was available. In this...

article

ISSR markers detect high genetic variation among Fusarium poae isolates from Argentina and England

María I. Dinolfo, Sebastian A. Stenglein, María V. Moreno, Paul Nicholson, more

European Journal of Plant Pathology > 2010 > 127 > 4 > 483-491

Fusarium poae is one of the Fusarium species isolated from cereal grains infected by Fusarium head blight (FHB), and in recent years it has been identified as a major FHB component. In this study, 97 F. poae isolates from Argentina (n = 62) and England (n = 35) were analysed by inter-simple sequence repeats (ISSR) to examine the genetic diversity and to determine whether intraspecific variation could...

article

The Cinderella story of sucrose hydrolysis: Alkaline/neutral invertases, from cyanobacteria to unforeseen roles in plant cytosol and organelles

Walter A. Vargas, Graciela L. Salerno

Plant Science > 2010 > 178 > 1 > 1-8

Over the past decades, considerable advances have been made in understanding the crucial role of sucrose and the regulation of its metabolism in plant life. Recent studies in cyanobacteria and the analysis of several genomic sequences point towards an ancient origin of plant sucrose metabolism before the cyanobacterial phylogenetic radiation. In agreement with the generally accepted cyanobacterial...

article

Role of NtcA, a cyanobacterial global nitrogen regulator, in the regulation of sucrose metabolism gene expression in Anabaena sp. PCC 7120

Clarisa Marcozzi, Andrea C. Cumino, Graciela L. Salerno

Archives of Microbiology > 2009 > 191 > 3 > 255-263

In the heterocyst-forming cyanobacterium Anabaena sp. PCC 7120 (also known as Nostoc sp. PCC 7120), it has been shown that spsB and susA, the genes coding for proteins related to sucrose synthesis and cleavage, respectively, exhibit converse expression regarding the nitrogen source. In the nitrogen-fixing filament, spsB expression is mostly localized to the heterocysts and susA is only expressed in...

article

Protein phosphatase activity and sucrose-mediated induction of fructan synthesis in wheat

Giselle M. A. Martínez-Noël, Jorge A. Tognetti, Graciela L. Salerno, Andres Wiemken, more

Planta > 2009 > 230 > 5 > 1071-1079

In this work, we analyze protein phosphatase (PP) involvement in the sucrose-mediated induction of fructan metabolism in wheat (Triticum aestivum). The addition of okadaic acid (OA), a PP-inhibitor, to sucrose-fed leaves reduced fructosylsucrose-synthesizing activity (FSS) induction in a dose-dependent manner. The expression of the two enzymes that contribute to FSS activity, 1-SST (1-sucrose:sucrose...

article

Sucrose synthase is involved in the conversion of sucrose to polysaccharides in filamentous nitrogen-fixing cyanobacteria

Leonardo Curatti, Laura E. Giarrocco, Andrea C. Cumino, Graciela L. Salerno

Planta > 2008 > 228 > 4 > 617-625

Higher plants and cyanobacteria metabolize sucrose (Suc) by a similar set of enzymes. Suc synthase (SuS, UDP-glucose: d-fructose 2-α-d-glucosyl transferase, EC 2.4.1.13) catalyses the synthesis and cleavage of Suc, and in higher plants, it plays an important role in polysaccharides biosynthesis and carbon allocation. In this work, we have studied the functional relationship between SuS and the metabolism...

article

New insights on sucrose metabolism: evidence for an active A/N-Inv in chloroplasts uncovers a novel component of the intracellular carbon trafficking

Walter A. Vargas, Horacio G. Pontis, Graciela L. Salerno

Planta > 2008 > 227 > 4 > 795-807

The presence of sucrose (Suc) in plastids was questioned for several decades. Although it was reported some decades ago, neither Suc transporters nor Suc metabolizing enzymes were demonstrated to be active in those organelles. By biochemical, immunological, molecular and genetic approaches we show that alkaline/neutral invertases (A/N-Invs) are also localized in chloroplasts of spinach and Arabidopsis...

article

Cloning and Characterization of a Novel Crystal Protein from a Native Bacillus thuringiensis Isolate Highly Active Against Aedes aegypti

Corina M. Berón, Graciela L. Salerno

Current Microbiology > 2007 > 54 > 4 > 271-276

We characterized a novel Bacillus thuringiensis isolate native to Argentina (FCC 41) that exhibits a mosquitocidal activity higher than the reference B. thuringiensis subsp. israelensis. This isolate shows a rounded crystal harboring two major proteins of about 70–80 kDa. Moreover, we cloned and sequenced the encoding gene of one of the crystal proteins (Cry) consisting of an open reading frame of...

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Keywords

CYANOBACTERIA (7)
SUCROSE METABOLISM (6)
SUCROSE-PHOSPHATE SYNTHASE (5)
ALKALINE/NEUTRAL INVERTASES (3)
SALT STRESS (3)
SUCROSE SYNTHASE (3)
SUCROSE-PHOSPHATE PHOSPHATASE (3)
ANABAENA SP. (2)
CYANOBACTERIAL BLOOMS (2)
CYANOBACTERIUM (2)
GLUCOSYLTRANSFERASE (2)
SALT TREATMENT (2)
SUCROSE HYDROLYSIS (2)
TRANSCRIPTIONAL REGULATION (2)
ANABAENA (1)
ANABAENA SP. (1)
ARABIDOPSIS (1)
BACILLUS THURINGIENSIS (1)
FUSARIUM POAE (1)
GLOEOBACTER VIOLACEUS (1)
MICROCYSTIS AERUGINOSA (1)
THERMOSYNECHOCOCCUS ELONGATUS (1)
TRITICUM (1)
ABIOTIC STRESS (1)
ALKALINE INVERTASE (1)
ALKALINE PHOSPHATASE (1)
ALKALINE/NEUTRAL INVERTASE (1)
AMMONIUM REGULATION (1)
AMYLOSUCRASE (1)
ANABAENA SP. (SUCROSE METABOLISM) CYANOBACTERIUM SUCROSE SUCROSE SYNTHASE (1)
ANABAENA SP. (SUCROSE METABOLISM) CYANOBACTERIUM SUCROSE METABOLISM SUCROSE SYNTHASE (1)
ANABAENA/NOSTOC (1)
ARABIDOPSIS THALIANA (1)
CALCIUM SIGNALLING (1)
CARBOHYDRATE METABOLISM (1)
CDPK (1)
CHLOROPLASTS (1)
COLD STRESS (1)
COMPATIBLE SOLUTES (1)
CYANOBACTERIUM SALT STRESS (1)
EUGLENA (1)
EUTROPHIC SHALLOW LAKE (1)
EUTROPHICATION (1)
FLOWERING (1)
FRUCTAN METABOLISM (1)
FRUCTANS (1)
FRUCTOKINASE (1)
FRUCTOSE-CONTAINING OLIGOSACCHARIDES (1)
FRUCTOSYLSUCROSE (1)
GENETIC VARIABILITY (1)
GERMINATION (1)
GLYCOGEN (1)
GLYCOGEN SYNTHESIS (1)
HETEROCYST-FORMING CYANOBACTERIA (1)
HYPOXIA (1)
INSECTICIDAL ACTIVITY (1)
INTER-SIMPLE SEQUENCE REPEATS (1)
INVASIVE SPECIES (1)
ISOLATION (1)
MITOCHONDRIAL ISOFORMS (1)
MODULAR ARRANGEMENT (1)
MODULAR PROTEIN (1)
MODULAR PROTEINS (1)
NATIVE STRAINS (1)
NEUTRAL/ALKALINE INVERTASES (1)
NIROGEN FIXATION (1)
NITROGEN METABOLISM (1)
NITROGEN STARVATION (1)
NTCA (1)
NUTRIENTS (1)
OKADAIC ACID (1)
OLIGOSACCHARIDES (1)
OSMOTIC STRESS (1)
OSTREOCOCCUS TAURI (1)
PHO REGULON (1)
PHOSPHOHYDROLASE (1)
PHYLOGENETIC ANALYSIS (1)
PHYTOPLANKTON DYNAMICS (1)
PRASINOPHYTES (1)
PROTEIN PHOSPHATASE (1)
RESPIRATION (1)
RIBULOSE BISPHOSPHATE CARBOXYLASE/OXYGENASE (1)
ROS (1)
SALT-RESPONSE (1)
SAXITOXIN (1)
SIGNALLING PATHWAYS (1)
SPECIES DIVERSITY (1)
SPINACH SUBCELLULAR LOCALIZATION (1)
SST, SUCROSE-SUCROSE FRUCTOSYL TRANSFERASE (1)
STRAIN COLLECTION (1)
STRESS RESPONSE (1)
SUBCELLULAR LOCALIZATION (1)
SUCROGLUCANS (1)
SUCROSE (1)
SUCROSE BIOSYNTHESIS (1)
SUCROSE CLEAVAGE (1)
SUCROSE CLUSTER (1)
SUCROSE SIGNALING (1)
SUCROSE SYNTHESIS (1)
SUCROSE UPTAKE (1)
more

Data set

Springer (19)
Elsevier (16)
Wiley (1)

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Search results for: Graciela L. Salerno

Target of rapamycin signaling is tightly and differently regulated in the plant response under distinct abiotic stresses

Raphidiopsis mediterranea (Nostocales) exhibits a flexible growth strategy under light and nutrient fluctuations in contrast to Planktothrix agardhii (Oscillatoriales)

Ancient signal for nitrogen status sensing in the green lineage: Functional evidence of CDPK repertoire in Ostreococcus tauri

Synergistic effects of nutrients and light favor Nostocales over non-heterocystous cyanobacteria

Sucrose in bloom‐forming cyanobacteria: loss and gain of genes involved in its biosynthesis

Functional characterization of Synechococcus amylosucrase and fructokinase encoding genes discovers two novel actors on the stage of cyanobacterial sucrose metabolism

First evidence of sucrose biosynthesis by single cyanobacterial bimodular proteins

The unique nucleotide specificity of the sucrose synthase from Thermosynechococcus elongatus

A mitochondrial alkaline/neutral invertase isoform (A/N-InvC) functions in developmental energy-demanding processes in Arabidopsis

Sucrose synthase in unicellular cyanobacteria and its relationship with salt and hypoxic stress

Genetic Diversity of cry Gene Sequences of Bacillus thuringiensis Strains Analyzed by Denaturing Gradient Gel Electrophoresis

Differential roles of alkaline/neutral invertases in Nostoc sp. PCC 7120: Inv-B isoform is essential for diazotrophic growth

The proteins involved in sucrose synthesis in the marine cyanobacterium Synechococcus sp. PCC 7002 are encoded by two genes transcribed from a gene cluster

ISSR markers detect high genetic variation among Fusarium poae isolates from Argentina and England

The Cinderella story of sucrose hydrolysis: Alkaline/neutral invertases, from cyanobacteria to unforeseen roles in plant cytosol and organelles

Role of NtcA, a cyanobacterial global nitrogen regulator, in the regulation of sucrose metabolism gene expression in Anabaena sp. PCC 7120

Protein phosphatase activity and sucrose-mediated induction of fructan synthesis in wheat

Sucrose synthase is involved in the conversion of sucrose to polysaccharides in filamentous nitrogen-fixing cyanobacteria

New insights on sucrose metabolism: evidence for an active A/N-Inv in chloroplasts uncovers a novel component of the intracellular carbon trafficking

Cloning and Characterization of a Novel Crystal Protein from a Native Bacillus thuringiensis Isolate Highly Active Against Aedes aegypti

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