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Phytase (EC 3.1.3.–) hydrolyzes phytate (IP 6 ) present in cereals and grains to release inorganic phosphate (P i ), thereby making it bioavailable. The most commonly used method to assay phytase, developed nearly a century ago, measures the P i liberated from IP 6 . This traditional endpoint assay is time-consuming and well known for its cumbersomeness in addition...
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