Search results for: Katarzyna Kulcenty

Items from 1 to 8 out of 8 results

article

Surgical wound fluids from patients treated with intraoperative radiotherapy induce radiobiological response in breast cancer cells

Igor Piotrowski, Katarzyna Kulcenty, Dawid Murawa, Wiktoria Suchorska

Medical Oncology > 2019 > 36 > 2 > 1-12

Breast cancer is the most common cancer occurring in women. The standard of breast cancer treatment is based on breast-conserving surgery with administration of adjuvant whole breast radiotherapy. Research shows that in-breast relapse is most likely to occur in the tumour bed, i.e. around the scar. Intraoperative radiotherapy (IORT), in which radiation is delivered to the tumour bed, reduces the risk...

article

Chondrogenic differentiation in vitro of hiPSCs activates pathways engaged in limb development

Ewelina Stelcer, Katarzyna Kulcenty, Marcin Rucinski, Karol Jopek, more

Stem Cell Research > 2018 > 30 > C > 53-60

Human induced pluripotent stem cells (hiPSCs) are a true breakthrough in regenerative medicine with the potential to successfully treat many diseases, including orthopedic lesions, that are unresponsive to current treatments. However, chondrogenic differentiation in vitro is a poorly understood process and more research is needed. In this study, we compared the gene expression profile of chondrocyte-like...

article

TRIM28 and Interacting KRAB-ZNFs Control Self-Renewal of Human Pluripotent Stem Cells through Epigenetic Repression of Pro-differentiation Genes

Urszula Oleksiewicz, Marta Gładych, Ayush T. Raman, Holger Heyn, more

Stem Cell Reports > 2017 > 9 > 6 > 2065-2080

Reprogramming to induced pluripotent stem cells (iPSCs) and differentiation of pluripotent stem cells (PSCs) are regulated by epigenetic machinery. Tripartite motif protein 28 (TRIM28), a universal mediator of Krüppel-associated box domain zinc fingers (KRAB-ZNFs), is known to regulate both processes; however, the exact mechanism and identity of participating KRAB-ZNF genes remain unknown. Here, using...

article

A feeder- and xeno-free human induced pluripotent stem cell line obtained from primary human dermal fibroblasts with epigenetic repression of reprogramming factors expression: GPCCi001-A

Michał Stefan Lach, Joanna P. Wroblewska, Ewelina Augustyniak, Katarzyna Kulcenty, more

Stem Cell Research > 2017 > 20 > C > 34-37

The primary human dermal fibroblasts (PHDFs) from breast cancer patient were obtained to generate the human induced pluripotent stem cell line GPCCi001-A via lentiviral transfection. Thus, a modified EF1a-hSTEMCCA-loxP with tetO operator which regulates transgene expression was used. This method takes advantage of epigenetic regulation of transcription and allows for stable silencing of the reprogramming...

article

Role of CYP1A1 in the biological activity of methylated resveratrol analogue, 3,4,5,4′-tetramethoxystilbene (DMU-212) in ovarian cancer A-2780 and non-cancerous HOSE cells

Hanna Piotrowska-Kempisty, Agnieszka Klupczyńska, Dorota Trzybulska, Katarzyna Kulcenty, more

Toxicology Letters > 2017 > 267 > C > 59-66

The role of CYP1A1 and CYP1B1 enzymes in the biotransformation and biological activity of the methylated resveratrol analogue, 3,4,5,4′-tetramethoxystilbene (DMU-212) is still elusive. Our recently published data have shown that one of the metabolites of DMU-212, 3′-hydroxy-3,4,5,4′-tetramethoxystilbene (DMU-214) exerts more potent cytotoxic effects in A-2780 ovarian cancer cell line, as compared...

article

Universal Real-Time PCR-Based Assay for Lentiviral Titration

Wojciech Barczak, Wiktoria Suchorska, Błażej Rubiś, Katarzyna Kulcenty

Molecular Biotechnology > 2015 > 57 > 2 > 195-200

Lentiviral vectors are efficient vehicles for stable gene transfer in both dividing and non-dividing cells. This feature among others makes lentiviral vectors a powerful tool in molecular research. However, the use of lentiviruses in research studies and clinical trials requires a precise and validated titration method. In this study, we describe a qPCR-based approach for estimation of lentiviral...

article

Different susceptibility of colon cancer DLD-1 and LOVO cell lines to apoptosis induced by DMU-212, a synthetic resveratrol analogue

Hanna Piotrowska, Krzysztof Myszkowski, Ryszard Amarowicz, Marek Murias, more

Toxicology in Vitro > 2013 > 27 > 8 > 2127-2134

The cytotoxic activity of DMU-212 has been shown to vary in cell lines derived from the same type of cancer, i.e. ovarian, breast and colorectal ones. However, the molecular mechanism of DMU-212 cytotoxicity has not been clarified in colon cancer cells. This study aims to elucidate the mechanism of antitumor effects of DMU-212 in two human colon cancer cell lines, DLD-1 and LOVO. We showed the stronger...

article

Resveratrol analogue 3,4,4′,5-tetramethoxystilbene inhibits growth, arrests cell cycle and induces apoptosis in ovarian SKOV‐3 and A-2780 cancer cells

Hanna Piotrowska, Krzysztof Myszkowski, Alicja Ziółkowska, Katarzyna Kulcenty, more

Toxicology and Applied Pharmacology > 2012 > 263 > 1 > 53-60

In the screening studies, cytotoxicity of 12 methylated resveratrol analogues on 11 human cancer cell lines was examined. The most active compound 3,4,4′5-tetramethoxystilbene (DMU-212) and two ovarian cancer cell lines A-2780 (IC ₅₀ =0.71μM) and SKOV-3 (IC ₅₀ =11.51μM) were selected for further investigation.To determine the mechanism of DMU-212 cytotoxicity, its ability to induce...

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Keywords

APOPTOSIS (2)
CELL CYCLE (2)
GENE EXPRESSION (2)
3,4,4′,5-TETRAMETHOXYSTILBENE (DMU-212) (1)
3,4,4′5-TETRAMETHOXYSTILBENE (1)
ALBUMIN (1)
BREAST CANCER (1)
CYP1A1 (1)
CYP1B1 (1)
DIFFERENTIATION (1)
DMU-212 (1)
DMU-214 (1)
DNA DAMAGE (1)
EPIGENETICS (1)
INDUCED PLURIPOTENT STEM CELLS (1)
INTRAOPERATIVE RADIOTHERAPY (1)
KRAB-ZNF REPRESSORS (1)
LENTIVIRUS (1)
METABOLIC ACTIVATION (1)
QPCR (1)
RADIATION-INDUCED BYSTANDER EFFECT (1)
REPROGRAMMING (1)
RESVERATROL (1)
TITRATION METHODS (1)
TRIM28 (1)
WOUND HEALING (1)
WPRE (1)
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Elsevier (6)
Springer (2)

INFONA - science communication portal

Search results for: Katarzyna Kulcenty

Surgical wound fluids from patients treated with intraoperative radiotherapy induce radiobiological response in breast cancer cells

Chondrogenic differentiation in vitro of hiPSCs activates pathways engaged in limb development

TRIM28 and Interacting KRAB-ZNFs Control Self-Renewal of Human Pluripotent Stem Cells through Epigenetic Repression of Pro-differentiation Genes

A feeder- and xeno-free human induced pluripotent stem cell line obtained from primary human dermal fibroblasts with epigenetic repression of reprogramming factors expression: GPCCi001-A

Role of CYP1A1 in the biological activity of methylated resveratrol analogue, 3,4,5,4′-tetramethoxystilbene (DMU-212) in ovarian cancer A-2780 and non-cancerous HOSE cells

Universal Real-Time PCR-Based Assay for Lentiviral Titration

Different susceptibility of colon cancer DLD-1 and LOVO cell lines to apoptosis induced by DMU-212, a synthetic resveratrol analogue

Resveratrol analogue 3,4,4′,5-tetramethoxystilbene inhibits growth, arrests cell cycle and induces apoptosis in ovarian SKOV‐3 and A-2780 cancer cells

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