Search results for: Kok-Lung Chan

Items from 1 to 9 out of 9 results

article

The Cohesin Ring Uses Its Hinge to Organize DNA Using Non-topological as well as Topological Mechanisms

Madhusudhan Srinivasan, Johanna C. Scheinost, Naomi J. Petela, Thomas G. Gligoris, more

Cell > 2018 > 173 > 6 > 1508-1519.e18

As predicted by the notion that sister chromatid cohesion is mediated by entrapment of sister DNAs inside cohesin rings, there is perfect correlation between co-entrapment of circular minichromosomes and sister chromatid cohesion. In most cells where cohesin loads without conferring cohesion, it does so by entrapment of individual DNAs. However, cohesin with a hinge domain whose positively charged...

article

Well-Characterised Time-Gated Detector Photon Flux Resolves the Ultrastructure of DNA-Damage Nuclear Bodies with G-STED Nanoscopy

Kok-Lung Chan, Esther Garcia Gonzalez, Sergi Padilla-Parra, Jorge Bernardino de la Serna

Biophysical Journal > 2017 > 112 > 3 Supplement 1 > 141a

article

Releasing Activity Disengages Cohesin’s Smc3/Scc1 Interface in a Process Blocked by Acetylation

Frederic Beckouët, Madhusudhan Srinivasan, Maurici Brunet Roig, Kok-Lung Chan, more

Molecular Cell > 2016 > 61 > 4 > 563-574

Sister chromatid cohesion conferred by entrapment of sister DNAs within a tripartite ring formed between cohesin’s Scc1, Smc1, and Smc3 subunits is created during S and destroyed at anaphase through Scc1 cleavage by separase. Cohesin’s association with chromosomes is controlled by opposing activities: loading by Scc2/4 complex and release by a separase-independent releasing activity as well as by...

article

Structure and function of cohesin’s Scc3/SA regulatory subunit

Maurici B. Roig, Jan Löwe, Kok-Lung Chan, Frédéric Beckouët, more

FEBS Letters > 2014 > 588 > 20 > 3692-3702

Sister chromatid cohesion involves entrapment of sister DNAs by a cohesin ring created through association of a kleisin subunit (Scc1) with ATPase heads of Smc1/Smc3 heterodimers. Cohesin’s association with chromatin involves subunits recruited by Scc1: Wapl, Pds5, and Scc3/SA, in addition to Scc2/4 loading complex. Unlike Pds5, Wapl, and Scc2/4, Scc3s are encoded by all eukaryotic genomes. Here,...

article

Cohesin’s DNA Exit Gate Is Distinct from Its Entrance Gate and Is Regulated by Acetylation

Kok-Lung Chan, Maurici B. Roig, Bin Hu, Frédéric Beckouët, more

Cell > 2012 > 150 > 5 > 961-974

Sister chromatid cohesion is mediated by entrapment of sister DNAs by a tripartite ring composed of cohesin’s Smc1, Smc3, and α-kleisin subunits. Cohesion requires acetylation of Smc3 by Eco1, whose role is to counteract an inhibitory (antiestablishment) activity associated with cohesin’s Wapl subunit. We show that mutations abrogating antiestablishment activity also reduce turnover of cohesin on...

article

New insights into the formation and resolution of ultra-fine anaphase bridges

Kok Lung Chan, Ian D. Hickson

Seminars in Cell and Developmental Biology > 2011 > 22 > 8 > 906-912

Recent data indicate an unexpected requirement for proteins that were hitherto considered to be dedicated to DNA repair to facilitate the faithful disjunction of sister chromatids in anaphase. These include the Bloom's syndrome gene product, BLM and its partners, as well as a number of proteins that are important for preventing Fanconi anemia (FA) in man. As part of an analysis of the roles of these...

article

ATP Hydrolysis Is Required for Relocating Cohesin from Sites Occupied by Its Scc2/4 Loading Complex

Bin Hu, Takehiko Itoh, Ajay Mishra, Yuki Katoh, more

Current Biology > 2011 > 21 > 1 > 12-24

The Cohesin complex that holds sister chromatins together until anaphase is comprised of three core subunits: Smc1 and Smc3, two long-rod-shaped proteins with an ABC-like ATPase head (nucleotide-binding domain [NBD]) and a dimerization domain linked by a 50 nm long intramolecular antiparallel coiled-coil, and Scc1, an α-kleisin subunit interconnecting the NBD domains of Smc1 and Smc3. Cohesin's stable...

article

High-throughput tissue microarray analysis of c-myc activation in chronic liver diseases and hepatocellular carcinoma

Kok-Lung Chan, Xin-Yuan Guan, Irene Oi-Lin Ng

Human Pathology > 2004 > 35 > 11 > 1324-1331

Amplification of 8q23-qter is common in human hepatocellular carcinoma (HCC). c-myc, an oncogene located on 8q24, may be important in hepatocarcinogenesis. The present study aimed to evaluate c-myc activation in hepatocarcinogenesis and its clinicopathological significance. High-throughput analysis of c-myc gene amplification and expression using dual-color fluorescence in situ hybridization and immunohistochemistry...

article

High frequency of chimerism in transplanted livers

Irene Oi-Lin Ng, Kok-Lung Chan, Wai-Hung Shek, Joyce Man-Fong Lee, more

Hepatology > 2003 > 38 > 4 > 989-998

Recent studies have shown that primitive stem cells can mobilize and differentiate into hepatocytes. We investigated the time and extent in which cells of recipient origins could differentiate into hepatocytes and other cells in human liver allografts. Microsatellite analysis, which can assess quantitatively the proportions of recipient and donor DNA, was performed in posttransplantation liver biopsy...

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