Search results for: Heng-Phon Too

Items from 1 to 20 out of 44 results

article

A “plug‐n‐play” modular metabolic system for the production of apocarotenoids

Congqiang Zhang, Xixian Chen, Nic D. Lindley, Heng‐Phon Too

Biotechnology and Bioengineering > 115 > 1 > 174 - 183

Apocarotenoids, such as α‐, β‐ionone, and retinol, have high commercial values in the food and cosmetic industries. The demand for natural ingredients has been increasing dramatically in recent years. However, attempts to overproduce β‐ionone in microorganisms have been limited by the complexity of the biosynthetic pathway. Here, an Escherichia coli‐based modular system was developed to produce various...

article

Efflux transporter engineering markedly improves amorphadiene production in Escherichia coli

Congqiang Zhang, Xixian Chen, Gregory Stephanopoulos, Heng‐Phon Too

Biotechnology and Bioengineering > 113 > 8 > 1755 - 1763

Metabolic engineering aims at altering cellular metabolism to produce valuable products at high yields and titers. Achieving high titers and productivity can be challenging if final products are largely accumulated intracellularly. A potential solution to this problem is to facilitate the export of these substances from cells by membrane transporters. Amorphadiene, the precursor of antimalarial drug...

article

Experimental design-aided systematic pathway optimization of glucose uptake and deoxyxylulose phosphate pathway for improved amorphadiene production

Congqiang Zhang, Ruiyang Zou, Xixian Chen, Gregory Stephanopoulos, more

Applied Microbiology and Biotechnology > 2015 > 99 > 9 > 3825-3837

Artemisinin is a potent antimalarial drug; however, it suffers from unstable and insufficient supply from plant source. Here, we established a novel multivariate-modular approach based on experimental design for systematic pathway optimization that succeeded in improving the production of amorphadiene (AD), the precursor of artemisinin, in Escherichia coli . It was initially found...

article

Nano-topology guided neurite outgrowth in PC12 cells is mediated by miRNAs

He Cheng, Lihan Zhou, Bihan Li, Mei Zhu, more

Nanomedicine: Nanotechnology, Biology, and Medicine > 2014 > 10 > 8 > 1871-1875

MicroRNAs (miRNAs) are master regulators of gene expression at post-transcriptional level. The present study investigated the involvement of miRNAs in topological guidance of neurite outgrowth in an NGF treated PC12 cell model cultured on nano-patterned polyethylene terephthalate (PET) substrates fabricated with interference lithography. The expressions of 38 neuronal miRNAs were measured and 3 were...

article

112 Identification and Validation of Serum MicroRNA Biomarker for Early Detection of Gastric Cancer

Lihan Zhou, Jimmy B. So, Ruiyang Zou, Zhu Feng, more

Gastroenterology > 2014 > 146 > 5 Supplement 1 > S-31

article

High-frequency stimulation of the globus pallidus interna nucleus modulates GFRα1 gene expression in the basal ganglia

Duncun Xun Kiat Ho, Yong Chee Tan, Jiayi Tan, Heng Phon Too, more

Journal of Clinical Neuroscience > 2014 > 21 > 4 > 657-660

Deep brain stimulation (DBS) is an established therapy for movement disorders such as Parkinson’s disease (PD). Although the efficacy of DBS is clear, its precise molecular mechanism remains unknown. The glial cell line derived factor (GDNF) family of ligands has been shown to confer neuroprotective effects on dopaminergic neurons, and putaminal infusion of GDNF have been investigated in PD patients...

article

MicroRNA as potential modulators in chemoresistant high-grade gliomas

Sharon Yin Yee Low, Yoon Khei Ho, Heng-Phon Too, Celestial Therese Yap, more

Journal of Clinical Neuroscience > 2014 > 21 > 3 > 395-400

Gliomas account for 70% of human malignant primary brain tumours. The most common form is glioblastoma multiforme, World Health Organization grade IV. Despite the implementation of post-operative adjuvant radiotherapy with concurrent temozolomide (TMZ), the disease’s overall prognosis remains dismal. TMZ is currently the only mono-chemotherapeutic agent for newly-diagnosed high-grade glioma patients...

article

Spatially resolved microrheology of heterogeneous biopolymer hydrogels using covalently bound microspheres

Long Hui Wong, Nicholas A. Kurniawan, Heng-Phon Too, Raj Rajagopalan

Biomechanics and Modeling in Mechanobiology > 2014 > 13 > 4 > 839-849

Characterization of the rheological properties of heterogeneous biopolymers is important not only to understand the effect of substrate elasticity on cell behaviors, but also to provide insights into mechanical changes during cellular remodeling of the environment. Conventional particle-tracking microrheology (PTM) techniques are compromised by probe–network slippage and cage-hopping problems, and...

article

GDNF family ligand dependent STAT3 activation is mediated by specific alternatively spliced isoforms of GFRα2 and RET

Lihan Zhou, Heng-Phon Too

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research > 2013 > 1833 > 12 > 2789-2802

Neurturin (NRTN), a member of the GDNF family of ligands (GFL), is currently investigated in a series of clinical trials for Parkinson's disease. NRTN signals through its cognate receptor GFRα2 and co-receptor RET to induce neurite outgrowth, but the underlying mechanism remains to be better understood. STAT3 was previously shown to be activated by oncogenic RET, independent of ligand and GFRα. In...

article

Optimization of amorphadiene synthesis in bacillus subtilis via transcriptional, translational, and media modulation

Kang Zhou, Ruiyang Zou, Congqiang Zhang, Gregory Stephanopoulos, more

Biotechnology and Bioengineering > 110 > 9 > 2556 - 2561

Genetically engineered microbes have been intensively investigated as a means for the cost‐effective production of isoprenoids. Bacillus subtilis is a promising microbial host for this purpose because of its fast growth rate and GRAS (generally regarded as safe) status. To date, development of this host has been impaired by the lack of genetic tools for modulating the expression of multiple genes...

article

Cyclic AMP signalling through PKA but not Epac is essential for neurturin-induced biphasic ERK1/2 activation and neurite outgrowths through GFRα2 isoforms

Guoqiang Wan, Lihan Zhou, Qing 'En Lim, Yung Hou Wong, more

Cellular Signalling > 2011 > 23 > 11 > 1727-1737

Cyclic AMP (cAMP) and neurotrophic factors are known to interact closely to promote neurite outgrowth and neuronal regeneration. Glial cell line-derived neurotrophic factor (GDNF) and its family member neurturin (NTN) transduce signal through a multi-component receptor complex consisting of GDNF family receptor alpha 2 (GFRα2) and Ret receptor tyrosine kinase. Neurons from GFRα2-deficient mice do...

article

Gold decorated NaYF₄:Yb,Er/NaYF₄/silica (core/shell/shell) upconversion nanoparticles for photothermal destruction of BE(2)-C neuroblastoma cells

Li Peng Qian, Li Han Zhou, Heng-Phon Too, Gan-Moog Chow

Journal of Nanoparticle Research > 2011 > 13 > 2 > 499-510

Gold decorated NaYF₄:Yb,Er/NaYF₄/silica (core/shell/shell) upconversion (UC) nanoparticles (~70–80 nm) were synthesized using tetraethyl orthosilicate and chloroauric acid in a one-step reverse microemulsion method. Gold nanoparticles (~6 nm) were deposited on the surface of silica shell of these core/shell/shell nanoparticles. The total upconversion emission intensity (green, red, and blue) of the...

article

A specific isoform of glial cell line‐derived neurotrophic factor family receptor alpha 1 regulates RhoA expression and glioma cell migration

Guoqiang Wan, Heng‐Phon Too

Journal of Neurochemistry > 115 > 3 > 759 - 770

J. Neurochem. (2010) 115, 759–770. AbstractMalignant gliomas are highly invasive neuroepithelial tumors where the tendency to invade and migrate away from the primary tumor mass is thought to be a leading cause of tumor recurrence and treatment failures. Autocrine signals produced by secreted factors that signal through receptors on the tumor are known to contribute to the invasiveness. Glial cell...

article

Identification and validation of reference genes for expression studies in a rat model of neuropathic pain

Guoqiang Wan, Kai Yang, Qing ‘En Lim, Lihan Zhou, more

Biochemical and Biophysical Research Communications > 2010 > 400 > 4 > 575-580

Neuropathic pain is triggered by damage to or as a result of the dysfunction of the somatosensory nervous system. Gene expression profiling using DNA microarray and real-time PCR have emerged as powerful tools for the elucidation of pain-specific pathways and identification of candidate biomarkers and therapeutic targets. Proper normalization of the gene expression data with stable reference genes...

article

Evaluation of Pre-Analytical Variables in the Quantification of Dengue Virus by Real-Time Polymerase Chain Reaction

Azlinda Anwar, Guoqiang Wan, Kaw-Bing Chua, Joseph Thomas August, more

The Journal of Molecular Diagnostics > 2009 > 11 > 6 > 537-542

An accurate molecular diagnosis for viral pathogens is highly dependent on pre-analytical procedures. The efficiencies of two viral RNA extraction methods (liquid phase partition and silica-based adsorption chromatography) and the effects of handling and storage on the stability of RNA isolated from dengue virus (DENV) were studied. Viral RNA extracted from spiked sera or clinical samples characterized...

article

GDNF-induced cell signaling and neurite outgrowths are differentially mediated by GFRalpha1 isoforms

Li Foong Yoong, Guoqiang Wan, Heng-Phon Too

Molecular and Cellular Neuroscience > 2009 > 41 > 4 > 464-473

Glial cell line-derived neurotrophic factor (GDNF) transduces signal and promotes neurite outgrowths in diverse neurons through the interactions of GDNF family receptor alpha 1 (GFRα1) and other co-receptors including Ret receptor tyrosine kinase and NCAM. GFRα1 is alternatively spliced into two isoforms, GFRα1a and GFRα1b, with five amino acids difference. In this study, we found that both GFRα1a...

article

Glial cell-line derived neurotrophic factor (GDNF) family of ligands confer chemoresistance in a ligand-specific fashion in malignant gliomas

Wai Hoe Ng, Guo Qiang Wan, Zhong Ni Peng, Heng Phon Too

Journal of Clinical Neuroscience > 2009 > 16 > 3 > 427-436

Glial cell-line derived neurotrophic factor (GDNF) is a neurotrophic factor known to promote neuronal survival of dopaminergic neurons in the embryonic midbrain as well as contribute to carcinogenesis in many cancers. Its ubiquitous presence in the central nervous system suggests a role in the mitogenesis of high-grade astrocytoma. GDNF is overexpressed in glioblastoma cell lines and human gliomas...

article

Carboxyl-Terminated Dendrimer-Coated Bioactive Interface for Protein Microarray: High-Sensitivity Detection of Antigen in Complex Biological Samples

Parayil Kumaran Ajikumar, Jin Kiat Ng, Yew Chung Tang, Jim Yang Lee, more

Langmuir > 2007 > 23 > 10 > 5670 - 5677

article

Dissociation of double-stranded DNA by small metal nanoparticles

Jun Yang, Boon-Kin Pong, Jim Yang Lee, Heng-Phon Too

Journal of Inorganic Biochemistry > 2007 > 101 > 5 > 824-830

The dissociation of double-stranded DNA (dsDNA) by 5nm Au nanoparticles was observed through a series of DNA melting transition measurements. Experimental evidence implicates a strong non-specific interaction between the dsDNA and small Au nanoparticles as the cause. Subsequently the relative affinities of mononucleotides and polynucleotides for the 5nm Au nanoparticle were determined by measuring...

article

A general phase transfer protocol for synthesizing alkylamine-stabilized nanoparticles of noble metals

J. Yang, Jim Yang Lee, Heng-Phon Too

Analytica Chimica Acta > 2007 > 588 > 1 > 34-41

The ethanol-mediated phase transfer protocol was extended herein to prepare alkylamine-stabilized nanoparticles of several noble metals by transferring them from aqueous environment into toluene. This method relies on the use of ethanol as a mediator to provide and maintain adequate contact between dodecylamine and metal nanoparticles during the transfer process and involves first mixing the metal...

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Search results for: Heng-Phon Too

A “plug‐n‐play” modular metabolic system for the production of apocarotenoids

Efflux transporter engineering markedly improves amorphadiene production in Escherichia coli

Experimental design-aided systematic pathway optimization of glucose uptake and deoxyxylulose phosphate pathway for improved amorphadiene production

Nano-topology guided neurite outgrowth in PC12 cells is mediated by miRNAs

112 Identification and Validation of Serum MicroRNA Biomarker for Early Detection of Gastric Cancer

High-frequency stimulation of the globus pallidus interna nucleus modulates GFRα1 gene expression in the basal ganglia

MicroRNA as potential modulators in chemoresistant high-grade gliomas

Spatially resolved microrheology of heterogeneous biopolymer hydrogels using covalently bound microspheres

GDNF family ligand dependent STAT3 activation is mediated by specific alternatively spliced isoforms of GFRα2 and RET

Optimization of amorphadiene synthesis in bacillus subtilis via transcriptional, translational, and media modulation

Cyclic AMP signalling through PKA but not Epac is essential for neurturin-induced biphasic ERK1/2 activation and neurite outgrowths through GFRα2 isoforms

Gold decorated NaYF₄:Yb,Er/NaYF₄/silica (core/shell/shell) upconversion nanoparticles for photothermal destruction of BE(2)-C neuroblastoma cells

A specific isoform of glial cell line‐derived neurotrophic factor family receptor alpha 1 regulates RhoA expression and glioma cell migration

Identification and validation of reference genes for expression studies in a rat model of neuropathic pain

Evaluation of Pre-Analytical Variables in the Quantification of Dengue Virus by Real-Time Polymerase Chain Reaction

GDNF-induced cell signaling and neurite outgrowths are differentially mediated by GFRalpha1 isoforms

Glial cell-line derived neurotrophic factor (GDNF) family of ligands confer chemoresistance in a ligand-specific fashion in malignant gliomas

Carboxyl-Terminated Dendrimer-Coated Bioactive Interface for Protein Microarray: High-Sensitivity Detection of Antigen in Complex Biological Samples

Dissociation of double-stranded DNA by small metal nanoparticles

A general phase transfer protocol for synthesizing alkylamine-stabilized nanoparticles of noble metals

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