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The fate of thymic emigrants had so far been studied using a variety of markers, each of which had inherent limitations as to stability, toxicity, or selectivity. We describe a new technique which relies on thein vivoinjection of CFSE, an esterified vital dye hitherto used at 80 times lower concentrations forin vitrocell labeling. We show that CFSE labels a representative sample of all thymocyte subsets...