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The thrombin-like serine protease and antithrombotic agent. Ancrod, was rapidly purified from the crude venom of Akistradon rhodostoma by agmatine-Sepharose affinity chromatography followed by MonoQ anion exchange chromatography. N-Terminal sequencing and analysis of overlapping proteolytic fragments of purified Ancrod by automated Edman degradation in combination with tandem mass spectroscopy allowed...