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Lignocellulose—the dry matter of plants, or “plant biomass”—digestion is of increasing interest in organismal metabolism research, specifically the conversion of biomass into biofuels. Termites efficiently decompose lignocelluloses, and studies on lignocellulolytic systems may elucidate mechanisms of efficient lignocellulose degradation in termites as well as offer novel enzyme sources, findings which...
Site-directed mutagenesis of the endo-β-1,4-glucanase (EG) gene from the termite Reticulitermes speratus EG (RsEG) was performed to gain a better understanding of the role of certain amino acid residues in the activity of the enzyme. Three mutants, G91A, Y97W and K429A had higher activities towards carboxymethyl cellulose than the wild type. The mutations had synergistic effects since each single...
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