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The thiamine-auxotrophic yeast Yarrowia lipolytica 374/4 was selected as a promising producer of pyruvic acid (PA). The thiamine concentration in the medium was shown to have a crucial effect on PA production by this strain. Under limitation of yeast growth by thiamine, the cell requirement in thiamine for PA production depended on a source of carbon and energy used. Cultivation conditions were established...
During continuous cultivation of Yarrowia lipolytica N 1, oxygen requirements for growth and citric acid synthesis were found to depend on the iron concentration in the medium. A coupled effect of oxygen and iron concentrations on the functioning of the mitochondrial electron transport chain in Y. lipolytica N 1 was established. Based on the results obtained in continuous culture, conditions for citric...
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