Search results for: Robert A. Field

Items from 1 to 20 out of 92 results

article

The Effect of a Low Degree of Fluorine Substitution on Cotton Fiber Properties

Ofir Aharon Kuperman, Peterson Andrade, Tanguy Terlier, Jacob Judas Kain Kirkensgaard, more

Macromolecular Materials and Engineering > 309 > 3 > n/a - n/a

Cellulose modification often employs chemical processes to tailor its properties and functionalities to fit the demands of a wide range of applications, maximizing its potential as a versatile and sustainable material. From both synthetic and environmental standpoints, one of the ultimate goals is to achieve significant modifications to enhance the end properties of the cellulose while minimizing...

article

The Effect of a Low Degree of Fluorine Substitution on Cotton Fiber Properties

Ofir Aharon Kuperman, Peterson Andrade, Tanguy Terlier, Jacob Judas Kain Kirkensgaard, more

Macromolecular Materials and Engineering > 309 > 3 > n/a - n/a

Front Cover: The cover page of article 2300337 by Filipe Natalio and co‐workers shows the chemosynthesis of a glucose derivative depicted in the upper part of the illustration. This last compound is fed to a floating cotton ovule in vitro culture (central illustration), becoming integrated into the fibers (blue dots). These modified fibers show increased mechanical properties, as depicted with the...

article

Euglenatides, Potent Antiproliferative Cyclic Peptides Isolated from the Freshwater Photosynthetic Microalga Euglena gracilis

Mohammed Aldholmi, Rizwan Ahmad, Daniel Carretero‐Molina, Ignacio Pérez‐Victoria, more

Angewandte Chemie > 134 > 23 > n/a - n/a

By limiting the nitrogen source to glutamic acid, we isolated cyclic peptides from Euglena gracilis containing asparagine and non‐proteinogenic amino acids. Structure elucidation was accomplished through spectroscopic methods, mass spectrometry and chemical degradation. The euglenatides potently inhibit pathogenic fungi and cancer cell lines e.g., euglenatide B exhibiting IC₅₀ values of 4.3 μM in...

article

Euglenatides, Potent Antiproliferative Cyclic Peptides Isolated from the Freshwater Photosynthetic Microalga Euglena gracilis

Mohammed Aldholmi, Rizwan Ahmad, Daniel Carretero‐Molina, Ignacio Pérez‐Victoria, more

Angewandte Chemie International Edition > 61 > 23 > n/a - n/a

article

Molecular Recognition of Natural and Non‐Natural Substrates by Cellodextrin Phosphorylase from Ruminiclostridium Thermocellum Investigated by NMR Spectroscopy

Valeria Gabrielli, Juan C. Muñoz‐García, Giulia Pergolizzi, Peterson Andrade, more

Chemistry – A European Journal > 27 > 63 > 15688 - 15698

β‐1→4‐Glucan polysaccharides like cellulose, derivatives and analogues, are attracting attention due to their unique physicochemical properties, as ideal candidates for many different applications in biotechnology. Access to these polysaccharides with a high level of purity at scale is still challenging, and eco‐friendly alternatives by using enzymes in vitro are highly desirable. One prominent candidate...

article

Chemoenzymatic Synthesis of Fluorinated Cellodextrins Identifies a New Allomorph for Cellulose‐Like Materials**

Peterson Andrade, Juan C. Muñoz‐García, Giulia Pergolizzi, Valeria Gabrielli, more

Chemistry – A European Journal > 27 > 4 > 1374 - 1382

Understanding the fine details of the self‐assembly of building blocks into complex hierarchical structures represents a major challenge en route to the design and preparation of soft‐matter materials with specific properties. Enzymatically synthesised cellodextrins are known to have limited water solubility beyond DP9, a point at which they self‐assemble into particles resembling the antiparallel...

article

The structure of a GH149 β‐(1 → 3) glucan phosphorylase reveals a new surface oligosaccharide binding site and additional domains that are absent in the disaccharide‐specific GH94 glucose‐β‐(1 → 3)‐glucose (laminaribiose) phosphorylase

Sakonwan Kuhaudomlarp, Clare E. M. Stevenson, David M. Lawson, Robert A. Field

Proteins: Structure, Function, and Bioinformatics > 87 > 10 > 885 - 892

Glycoside phosphorylases (GPs) with specificity for β‐(1 → 3)‐gluco‐oligosaccharides are potential candidate biocatalysts for oligosaccharide synthesis. GPs with this linkage specificity are found in two families thus far—glycoside hydrolase family 94 (GH94) and the recently discovered glycoside hydrolase family 149 (GH149). Previously, we reported a crystallographic study of a GH94 laminaribiose...

article

Targeted photodynamic therapy of breast cancer cells using lactose-phthalocyanine functionalized gold nanoparticles

Paula García Calavia, Isabelle Chambrier, Michael J. Cook, Alan H. Haines, more

Journal of Colloid and Interface Science > 2018 > 512 > C > 249-259

Gold nanoparticles (AuNPs), which have been widely used for the delivery of photosensitizers for photodynamic therapy (PDT) of cancer, can be dispersed in aqueous solutions improving the delivery of the hydrophobic photosensitizer into the body. Furthermore, the large surface of AuNPs can be functionalized with a variety of ligands, including proteins, nucleic acids and carbohydrates, that allow selective...

article

Heterologous co-expression in E. coli of isoamylase genes from cassava Manihot esculenta Crantz ‘KU50’ achieves enzyme-active heteromeric complex formation

Pawinee Panpetch, Robert A. Field, Tipaporn Limpaseni

Plant Molecular Biology > 2018 > 96 > 4-5 > 417-427

Key message Cloning of two isoamylase genes, MeISA1 and MeISA2, from cassava (Manihot esculenta Crantz) tubers, accompanied by their co-expression in E. coli demonstrates a requirement for heteromeric complex formation to achieve debranching activity. Abstract Starch debranching enzyme (DBE) or isoamylase (ISA) (EC.3.2.1.68), an important enzyme in starch metabolism, catalyses the hydrolysis of...

article

Structural characterisation of the capsular polysaccharide expressed by Burkholderia thailandensis strain E555:: wbiI (pKnock-KmR) and assessment of the significance of the 2-O-acetyl group in immune protection

Marc Bayliss, Matthew I. Donaldson, Sergey A. Nepogodiev, Giulia Pergolizzi, more

Carbohydrate Research > 2017 > 452 > C > 17-24

Burkholderia pseudomallei and its close relative B. mallei are human pathogens that are classified as Tier 1 bio-threat agents. Both organisms have previously been shown to constitutively produce a capsular polysaccharide (CPS) that is both a virulence determinant and protective antigen. Extraction and purification of CPS for use as a potential vaccine candidate requires containment level 3 laboratories...

article

Cellodextrin phosphorylase from Ruminiclostridium thermocellum: X-ray crystal structure and substrate specificity analysis

Ellis C. O'Neill, Giulia Pergolizzi, Clare E.M. Stevenson, David M. Lawson, more

Carbohydrate Research > 2017 > 451 > C > 118-132

The GH94 glycoside hydrolase cellodextrin phosphorylase (CDP, EC 2.4.1.49) produces cellodextrin oligomers from short β-1→4-glucans and α-D-glucose 1-phosphate. Compared to cellobiose phosphorylase (CBP), which produces cellobiose from glucose and α-D-glucose 1-phosphate, CDP is biochemically less well characterised. Herein, we investigate the donor and acceptor substrate specificity of recombinant...

article

Membrane-enclosed multienzyme (MEME) synthesis of 2,7-anhydro-sialic acid derivatives

Marie Monestier, Dimitrios Latousakis, Andrew Bell, Sandra Tribolo, more

Carbohydrate Research > 2017 > 451 > C > 110-117

Naturally occurring 2,7-anhydro-alpha-N-acetylneuraminic acid (2,7-anhydro-Neu5Ac) is a transglycosylation product of bacterial intramolecular trans-sialidases (IT-sialidases). A facile one-pot two-enzyme approach has been established for the synthesis of 2,7-anhydro-sialic acid derivatives including those containing different sialic acid forms such as Neu5Ac and N-glycolylneuraminic acid (Neu5Gc)...

article

Glucose-1-phosphate uridylyltransferase from Erwinia amylovora: Activity, structure and substrate specificity

Stefano Benini, Mirco Toccafondi, Martin Rejzek, Francesco Musiani, more

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics > 2017 > 1865 > 11(PA) > 1348-1357

Erwinia amylovora, a Gram-negative plant pathogen, is the causal agent of Fire Blight, a contagious necrotic disease affecting plants belonging to the Rosaceae family, including apple and pear. E. amylovora is highly virulent and capable of rapid dissemination in orchards; effective control methods are still lacking. One of its most important pathogenicity factors is the exopolysaccharide amylovoran...

article

Fluorescent mannosides serve as acceptor substrates for glycosyltransferase and sugar-1-phosphate transferase activities in Euglena gracilis membranes

Irina M. Ivanova, Sergey A. Nepogodiev, Gerhard Saalbach, Ellis C. O'Neill, more

Carbohydrate Research > 2017 > 438 > C > 26-38

Synthetic hexynyl α-D-mannopyranoside and its α-1,6-linked disaccharide counterpart were fluorescently labelled through CuAAC click chemistry with 3-azido-7-hydroxycoumarin. The resulting triazolyl-coumarin adducts, which were amenable to analysis by TLC, HPLC and mass spectrometry, proved to be acceptor substrates for α-1,6-ManT activities in mycobacterial membranes, as well as α- and β-GalT activities...

chapter

Oligosaccharide Signalling Molecules

Robert A. Field

Plant-derived Natural Products > Biological activity > 349-359

The signalling potential of plant carbohydrates had largely been overlooked until the last 2 decades. Whether trehalose- and sucrose-derived compounds, or oligosaccharides emanating from cell wall fragmentation, carbohydrate signals are thought to play key roles in plant growth and development at one level, and in activating plant defences against microbial infection at another. However, the literature...

article

The impact of aminopyrene trisulfonate (APTS) label in acceptor glycan substrates for profiling plant pectin β-galactosyltransferase activities

Stephan Goetz, Martin Rejzek, Sergey A. Nepogodiev, Robert A. Field

Carbohydrate Research > 2016 > 433 > C > 97-105

Aminopyrene trisulfonate (APTS)-labelled disaccharides are demonstrated to serve as readily accessible acceptor substrates for galactosyltransferase activities present in Arabidopsis microsome preparations. The reductive amination procedure used to install the fluorophore results in loss of the ring structure of the reducing terminal sugar unit, such that a single intact sugar ring is present, attached...

article

Carbohydrate CuAAC click chemistry for therapy and diagnosis

Xiao-Peng He, Ya-Li Zeng, Yi Zang, Jia Li, more

Carbohydrate Research > 2016 > 429 > C > 1-22

Carbohydrates are important as signaling molecules and for cellular recognition events, therefore offering scope for the development of carbohydrate-mimetic diagnostics and drug candidates. As a consequence, the construction of carbohydrate-based bioactive compounds and sensors has become an active research area. While the advent of click chemistry has greatly accelerated the progress of medicinal...

article

CuAAC click chemistry with N-propargyl 1,5-dideoxy-1,5-imino-D-gulitol and N-propargyl 1,6-dideoxy-1,6-imino-D-mannitol provides access to triazole-linked piperidine and azepane pseudo-disaccharide iminosugars displaying glycosidase inhibitory properties

Luís Otávio B. Zamoner, Valquíria Aragão-Leoneti, Susimaire P. Mantoani, Michael D. Rugen, more

Carbohydrate Research > 2016 > 429 > C > 29-37

Protecting group-free synthesis of 1,2:5,6-di-anhydro-D-mannitol, followed by ring opening with propargylamine and subsequent ring closure produced a separable mix of piperidine N-propargyl 1,5-dideoxy-1,5-imino-D-gulitol and azepane N-propargyl 1,6-dideoxy-1,6-imino-D-mannitol. In O-acetylated form, these two building blocks were subjected to CuAAC click chemistry with a panel of three differently...

article

Validation and modelling of a novel diffusive sampler for determining concentrations of volatile organic compounds in air

Pascual Pérez Ballesta, Emanuela Grandesso, Robert A. Field, Ana Cabrerizo

Analytica Chimica Acta > 2016 > 908 > C > 102-112

A novel diffusive sampler that combines radial and axial diffusion has been developed that improves upon existing commercially available designs. The POcket Diffusive (POD) sampler has been validated under laboratory and field conditions for the measurements of VOCs in ambient air. Laboratory tests varied sampling conditions of temperature (−30–40 C), humidity (10–80%), wind velocity (0.1–4 m s⁻¹),...

article

Euglena in time: Evolution, control of central metabolic processes and multi-domain proteins in carbohydrate and natural product biochemistry

Ellis C. O’Neill, Martin Trick, Bernard Henrissat, Robert A. Field

Perspectives in Science > 2015 > 6 > C > 84-93

Euglena gracilis is a eukaryotic microalgae that has been the subject of scientific study for hundreds of years. It has a complex evolutionary history, with traces of at least four endosymbiotic genomes and extensive horizontal gene transfer. Given the importance of Euglena in terms of evolutionary cell biology and its unique taxonomic position, we initiated a de novo transcriptome sequencing project...

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Search results for: Robert A. Field

The Effect of a Low Degree of Fluorine Substitution on Cotton Fiber Properties

The Effect of a Low Degree of Fluorine Substitution on Cotton Fiber Properties

Euglenatides, Potent Antiproliferative Cyclic Peptides Isolated from the Freshwater Photosynthetic Microalga Euglena gracilis

Euglenatides, Potent Antiproliferative Cyclic Peptides Isolated from the Freshwater Photosynthetic Microalga Euglena gracilis

Molecular Recognition of Natural and Non‐Natural Substrates by Cellodextrin Phosphorylase from Ruminiclostridium Thermocellum Investigated by NMR Spectroscopy

Chemoenzymatic Synthesis of Fluorinated Cellodextrins Identifies a New Allomorph for Cellulose‐Like Materials**

The structure of a GH149 β‐(1 → 3) glucan phosphorylase reveals a new surface oligosaccharide binding site and additional domains that are absent in the disaccharide‐specific GH94 glucose‐β‐(1 → 3)‐glucose (laminaribiose) phosphorylase

Targeted photodynamic therapy of breast cancer cells using lactose-phthalocyanine functionalized gold nanoparticles

Heterologous co-expression in E. coli of isoamylase genes from cassava Manihot esculenta Crantz ‘KU50’ achieves enzyme-active heteromeric complex formation

Structural characterisation of the capsular polysaccharide expressed by Burkholderia thailandensis strain E555:: wbiI (pKnock-KmR) and assessment of the significance of the 2-O-acetyl group in immune protection

Cellodextrin phosphorylase from Ruminiclostridium thermocellum: X-ray crystal structure and substrate specificity analysis

Membrane-enclosed multienzyme (MEME) synthesis of 2,7-anhydro-sialic acid derivatives

Glucose-1-phosphate uridylyltransferase from Erwinia amylovora: Activity, structure and substrate specificity

Fluorescent mannosides serve as acceptor substrates for glycosyltransferase and sugar-1-phosphate transferase activities in Euglena gracilis membranes

Oligosaccharide Signalling Molecules

The impact of aminopyrene trisulfonate (APTS) label in acceptor glycan substrates for profiling plant pectin β-galactosyltransferase activities

Carbohydrate CuAAC click chemistry for therapy and diagnosis

CuAAC click chemistry with N-propargyl 1,5-dideoxy-1,5-imino-D-gulitol and N-propargyl 1,6-dideoxy-1,6-imino-D-mannitol provides access to triazole-linked piperidine and azepane pseudo-disaccharide iminosugars displaying glycosidase inhibitory properties

Validation and modelling of a novel diffusive sampler for determining concentrations of volatile organic compounds in air

Euglena in time: Evolution, control of central metabolic processes and multi-domain proteins in carbohydrate and natural product biochemistry

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