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Abstract. Motifs for sequence specific-protein-DNA interactions, such as helix-turn-helix, zinc finger and leucine zipper, are now better understood as a result of extensive studies of three-dimensional (3D) structures of transcription factors. On the other hand, little attention has been paid to motifs for sequence nonspecific binding, namely DNA-phosphate binding. To address the question whether...
Purpose: The origin of introns is a highly controversial question. Whether introns existed in ancestral genes of prokaryote or they were inserted later into the genes of eukaryotes remains unanswered. Correlation of exons with structural units of ancient or widely distributed proteins will support the former view.Methods: We decomposed glucolitic enzymes into modules. Glycolytic enzymes were suitable...
Purpose: Protein-DNA base interaction is achieved by a number of structural motifs in proteins. Specificity is given by differences in amino acid residues of the motifs. What is, then, the situation of protein-DNA phosphate interaction, namely nonspecific interaction?Methods: Three-dimensional structures of proteins bound to DNA were decomposed into modules, compact structural units. Their structures...
The membranes are manufactured by immersing SiO 2 -containing substrates in an aqueous NaOH solution in the presence of aluminate ions to crystallize the zeolites. The NaOH aqueous solution may contain 0.5 mol of Na + and 0.02 mol of aluminate ion/l. The membranes are especially suitable for separating gases, gases and liquids, and as petroleum-refining catalysts.
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