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This is a first report of recombinant production of human prepro-Urocortin 2 in Escherichia coli by N-terminal fusion with a triple His 6 -SUMO-eXact tag and its subsequent use as an antigen for the production and screening of very high affinity monoclonal antibodies. The rationale for this combinatorial construct is that the His tag allows first step protein purification of insoluble and...
In a previous paper, we reported more efficient enterokinase cleavage at a C-terminal non-target LKGDR 201 site compared with an internally sited canonical recognition site, DDDDK 156 . When this non-target site was placed internally to replace DDDDK 156 between the thioredoxin moiety and mouse NT-proCNP(1–50), this site was poorly processed leading us to conclude that efficient...
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