Search results for: M. Focke

Items from 1 to 20 out of 42 results

article

Luminal A versus luminal B breast cancer: MammaTyper mRNA versus immunohistochemical subtyping with an emphasis on standardised Ki67 labelling‐based or mitotic activity index‐based proliferation assessment

Kai Finsterbusch, Thomas Decker, Paul J Diest, Cornelia M Focke

Aims Proliferation assessment by the use of Ki67 is a crucial component in intrinsic subtyping of luminal breast cancers (BCs), but suffers from variability between laboratories, observers, and methods. MammaTyper is a quantitative molecular tool that measures mRNA levels of ERBB2, ESR1, PGR and MKI67 in BC, and interprets the results according to the St Gallen 2013 consensus recommendations. We...

article

Analytical validation of a standardised scoring protocol for Ki67 immunohistochemistry on breast cancer excision whole sections: an international multicentre collaboration

Samuel C Y Leung, Torsten O Nielsen, Lila A Zabaglo, Indu Arun, more

Histopathology > 75 > 2 > 225 - 235

Aims The nuclear proliferation marker Ki67 assayed by immunohistochemistry has multiple potential uses in breast cancer, but an unacceptable level of interlaboratory variability has hampered its clinical utility. The International Ki67 in Breast Cancer Working Group has undertaken a systematic programme to determine whether Ki67 measurement can be analytically validated and standardised among laboratories...

article

Similar localization of conformational IgE epitopes on the house dust mite allergens Der p 5 and Der p 21 despite limited IgE cross‐reactivity

M. Curin, T. Garmatiuk, Y. Resch‐Marat, K. W. Chen, more

Allergy > 73 > 8 > 1653 - 1661

Background Due to high IgE recognition frequency and high allergenic activity, Der p 5 and Der p 21 are clinically important house dust mite (HDM) allergens. The objective of this study was to characterize the immunodominant IgE epitopes of Der p 5 and Der p 21 responsible for their high allergenic activity. Methods A panel of 12 overlapping peptides spanning the Der p 5 and Der p 21 sequence were...

article

Isolation of a high‐affinity Bet v 1‐specific IgG‐derived ScFv from a subject vaccinated with hypoallergenic Bet v 1 fragments

E. Gadermaier, K. Marth, C. Lupinek, R. Campana, more

Allergy > 73 > 7 > 1425 - 1435

Background Recombinant hypoallergenic allergen derivatives have been used in clinical immunotherapy studies, and clinical efficacy seems to be related to the induction of blocking IgG antibodies recognizing the wild‐type allergens. However, so far no treatment‐induced IgG antibodies have been characterized. Objective To clone, express, and characterize IgG antibodies induced by vaccination with...

article

BTK inhibition is a potent approach to block IgE‐mediated histamine release in human basophils

D. Smiljkovic, K. Blatt, G. Stefanzl, Y. Dorofeeva, more

Allergy > 72 > 11 > 1666 - 1676

Background Recent data suggest that Bruton's tyrosine kinase (BTK) is an emerging therapeutic target in IgE receptor (IgER)‐cross‐linked basophils. Methods We examined the effects of four BTK inhibitors (ibrutinib, dasatinib, AVL‐292, and CNX‐774) on IgE‐dependent activation and histamine release in blood basophils obtained from allergic patients (n=11) and nonallergic donors (n=5). In addition,...

article

Interlaboratory variability of Ki67 staining in breast cancer

Cornelia M. Focke, Horst Bürger, Paul J. van Diest, Kai Finsterbusch, more

European Journal of Cancer > 2017 > 84 > C > 219-227

Postanalytic issues of Ki67 assessment in breast cancers like counting method standardisation and interrater bias have been subject of various studies, but little is known about analytic variability of Ki67 staining between pathology labs. Our aim was to study interlaboratory variability of Ki67 staining in breast cancer using tissue microarrays (TMAs) and central assessment to minimise preanalytic...

article

Pru p 3, a marker allergen for lipid transfer protein sensitization also in Central Europe

N. Mothes‐Luksch, M. Raith, G. Stingl, M. Focke‐Tejkl, more

Allergy > 72 > 9 > 1415 - 1418

In the Mediterranean area, lipid transfer proteins (LTPs) are important causes of plant‐food allergies often associated with severe allergic reactions. There, peach LTP (Pru p 3) seems to be the primary sensitizer, whereas in Central Europe, little is known about the importance of LTP sensitization. In this region, allergen extract‐based diagnosis is often complicated by co‐sensitization to Bet v...

article

Infant milk formulas differ regarding their allergenic activity and induction of T‐cell and cytokine responses

H. Hochwallner, U. Schulmeister, I. Swoboda, M. Focke‐Tejkl, more

Allergy > 72 > 3 > 416 - 424

Background Several hydrolyzed cow's milk (CM) formulas are available for avoidance of allergic reactions in CM‐allergic children and for prevention of allergy development in high‐risk infants. Our aim was to compare CM formulas regarding the presence of immunoreactive CM components, IgE reactivity, allergenic activity, ability to induce T‐cell proliferation, and cytokine secretion. Methods A blinded...

article

Reliability of the Ki67-Labelling Index in Core Needle Biopsies of Luminal Breast Cancers is Unaffected by Biopsy Volume

C. M. Focke, T. Decker, P. J. Diest

Annals of Surgical Oncology > 2017 > 24 > 5 > 1251-1257

Background Assessing prognostic and predictive factors like the Ki67 labelling index (Ki67-LI) in breast cancer core needle biopsies (CNB) may be hampered by undersampling. Our aim was to arrive at a representative assessment of Ki67-LI in CNB of luminal breast cancers by defining optimal cutoffs and establishing the minimum CNB volume needed for highest concordance of Ki67-LI between CNB and subsequent...

article

The reliability of histological grade in breast cancer core needle biopsies depends on biopsy size: a comparative study with subsequent surgical excisions

Cornelia M Focke, Thomas Decker, Paul J Diest

Histopathology > 69 > 6 > 1047 - 1054

Aims In breast cancer patients undergoing neoadjuvant chemotherapy, histological grading needs to be performed on core needle biopsies (CNBs) that may not be representative of the whole tumour when they are small. Our aim was to study the influence of biopsy size on agreement rates for histological grade between CNBs and subsequent surgical excision biopsies (SEBs). Methods and results We calculated...

article

Intratumoral heterogeneity of Ki67 expression in early breast cancers exceeds variability between individual tumours

Cornelia M Focke, Thomas Decker, Paul J Diest

Histopathology > 69 > 5 > 849 - 861

AimsRegional differences in proliferative activity are commonly seen within breast cancers, but little is known on the extent of intratumoral heterogeneity of Ki67 expression. Our aim was to study the intratumoral heterogeneity of Ki67 expression in early breast cancers and its association with clinicopathological features, such as oestrogen receptor (ER) status, grade and histological subtype. Methods...

article

St Gallen 2015 subtyping of luminal breast cancers: impact of different Ki67-based proliferation assessment methods

Cornelia M. Focke, Paul J. Diest, Thomas Decker

Breast Cancer Research and Treatment > 2016 > 159 > 2 > 257-263

Ki67 has been proposed as prognostic proliferation marker in luminal breast cancer (BC), but little is known on the influence of Ki67 assessment methods on subtyping into luminal A- and B-like tumors. Our aim was to study the influence of different Ki67-labeling index (Ki67-LI) assessment methods on the proportion of BCs classified as luminal A-like. 280 early BCs were subtyped according to the St...

article

Epitope specificity determines cross‐protection of a SIT‐induced IgG₄ antibody

E. Gadermaier, L. K. James, M. H. Shamji, K. Blatt, more

Allergy > 71 > 1 > 36 - 46

BackgroundThe calcium‐binding 2EF‐hand protein Phl p 7 from timothy grass pollen is a highly cross‐reactive pollen pan‐allergen that can induce severe clinical symptoms in allergic patients. Recently, a human monoclonal Phl p 7‐specific IgG₄ antibody (mAb102.1F10) was isolated from a patient who had received grass pollen‐specific immunotherapy (SIT). MethodsWe studied epitope specificity, cross‐reactivity,...

article

Natural clinical tolerance to peanut in African patients is caused by poor allergenic activity of peanut IgE

E. Wollmann, C. Hamsten, E. Sibanda, M. Ochome, more

Allergy > 70 > 6 > 638 - 652

BackgroundIn Africa, peanuts are frequently consumed, but severe allergic reactions are rare. We investigated immunological patterns of clinical tolerance to peanut in peanut‐sensitized but asymptomatic patients from central Africa compared to peanut‐allergic and peanut‐sensitized but asymptomatic patients from Sweden. MethodsSera from allergic patients (n = 54) from Zimbabwe sensitized to peanut...

article

Multiple independent IgE epitopes on the highly allergenic grass pollen allergen Phl p 5

M. Levin, S. Rotthus, S. Wendel, N. Najafi, more

Clinical & Experimental Allergy > 44 > 11 > 1409 - 1419

BackgroundGroup 5 allergens are small proteins that consist of two domains. They belong to the most potent respiratory allergens. ObjectiveTo determine the binding sites and to study allergic patients' IgE recognition of the group 5 allergen (Phl p 5) from timothy grass pollen using human monoclonal IgE antibodies that have been isolated from grass pollen allergic patients. MethodsUsing recombinant...

article

Hypoallergenic derivatives of Fel d 1 obtained by rational reassembly for allergy vaccination and tolerance induction

M. Curin, M. Weber, T. Thalhamer, I. Swoboda, more

Clinical & Experimental Allergy > 44 > 6 > 882 - 894

Background and objectiveThe major cat allergen Fel d 1 represents one of the most important respiratory allergens. Aim of this study was to engineer recombinant Fel d 1 derivatives with reduced IgE reactivity and preserved T cell epitopes for vaccination and tolerance induction. MethodsSeven recombinant mosaic proteins were generated by reassembly of non‐IgE‐reactive peptides of Fel d 1 which contained...

article

Prophylactic and therapeutic vaccination with carrier‐bound Bet v 1 peptides lacking allergen‐specific T cell epitopes reduces Bet v 1‐specific T cell responses via blocking antibodies in a murine model for birch pollen allergy

B. Linhart, M. Narayanan, M. Focke‐Tejkl, F. Wrba, more

Clinical & Experimental Allergy > 44 > 2 > 278 - 287

BackgroundVaccines consisting of allergen‐derived peptides lacking IgE reactivity and allergen‐specific T cell epitopes bound to allergen‐unrelated carrier molecules have been suggested as candidates for allergen‐specific immunotherapy. ObjectiveTo study whether prophylactic and therapeutic vaccination with carrier‐bound peptides from the major birch pollen allergen Bet v 1 lacking allergen‐specific...

article

Hydrolysis and Anaerobic Co-Fermentation of Different Kinds of Biodegradable Polymers

V. Grundmann, B. Bilitewski, A. Zentner, C.-R. Wonschik, more

Waste and Biomass Valorization > 2013 > 4 > 2 > 371-376

Recent developments in the utilization of biowaste show that in future it will be more and more treated by a combination of energetic and material utilization in fermentation plants with a subsequent composting plant or a combination of both. In some regions in Germany the public sector waste disposal organisations already recommend the usage of waste bags made of biodegradable polymers. There is...

article

Carrier‐bound Alt a 1 peptides without allergenic activity for vaccination against Alternaria alternata allergy

T. E. Twaroch, M. Focke, K. Fleischmann, N. Balic, more

Clinical & Experimental Allergy > 42 > 6 > 966 - 975

BackgroundThe mould Alternaria alternata is a major elicitor of allergic asthma. Diagnosis and specific immunotherapy (SIT) of Alternaria allergy are often limited by the insufficient quality of natural mould extracts. ObjectiveTo investigate whether recombinant Alt a 1 can be used for reliable diagnosis of Alternaria alternata allergy and to develop a safe, non‐allergenic vaccine for SIT of Alternaria...

article

Carrier‐bound nonallergenic Der p 2 peptides induce IgG antibodies blocking allergen‐induced basophil activation in allergic patients

K.‐W. Chen, M. Focke‐Tejkl, K. Blatt, M. Kneidinger, more

Allergy > 67 > 5 > 609 - 621

BackgroundMore than 90% of house dust mite‐allergic patients are sensitized to the major Dermatophagoides pteronyssinus allergen, Der p 2. The aim of this study was to develop and characterize an allergy vaccine based on carrier‐bound Der p 2 peptides, which should allow reducing IgE‐ and T‐cell‐mediated side‐effects during specific immunotherapy (SIT). MethodsFive Der p 2 peptides (P1–P5) were synthesized...

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Search results for: M. Focke

Luminal A versus luminal B breast cancer: MammaTyper mRNA versus immunohistochemical subtyping with an emphasis on standardised Ki67 labelling‐based or mitotic activity index‐based proliferation assessment

Analytical validation of a standardised scoring protocol for Ki67 immunohistochemistry on breast cancer excision whole sections: an international multicentre collaboration

Similar localization of conformational IgE epitopes on the house dust mite allergens Der p 5 and Der p 21 despite limited IgE cross‐reactivity

Isolation of a high‐affinity Bet v 1‐specific IgG‐derived ScFv from a subject vaccinated with hypoallergenic Bet v 1 fragments

BTK inhibition is a potent approach to block IgE‐mediated histamine release in human basophils

Interlaboratory variability of Ki67 staining in breast cancer

Pru p 3, a marker allergen for lipid transfer protein sensitization also in Central Europe

Infant milk formulas differ regarding their allergenic activity and induction of T‐cell and cytokine responses

Reliability of the Ki67-Labelling Index in Core Needle Biopsies of Luminal Breast Cancers is Unaffected by Biopsy Volume

The reliability of histological grade in breast cancer core needle biopsies depends on biopsy size: a comparative study with subsequent surgical excisions

Intratumoral heterogeneity of Ki67 expression in early breast cancers exceeds variability between individual tumours

St Gallen 2015 subtyping of luminal breast cancers: impact of different Ki67-based proliferation assessment methods

Epitope specificity determines cross‐protection of a SIT‐induced IgG₄ antibody

Natural clinical tolerance to peanut in African patients is caused by poor allergenic activity of peanut IgE

Multiple independent IgE epitopes on the highly allergenic grass pollen allergen Phl p 5

Hypoallergenic derivatives of Fel d 1 obtained by rational reassembly for allergy vaccination and tolerance induction

Prophylactic and therapeutic vaccination with carrier‐bound Bet v 1 peptides lacking allergen‐specific T cell epitopes reduces Bet v 1‐specific T cell responses via blocking antibodies in a murine model for birch pollen allergy

Hydrolysis and Anaerobic Co-Fermentation of Different Kinds of Biodegradable Polymers

Carrier‐bound Alt a 1 peptides without allergenic activity for vaccination against Alternaria alternata allergy

Carrier‐bound nonallergenic Der p 2 peptides induce IgG antibodies blocking allergen‐induced basophil activation in allergic patients

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