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Cathepsin D is the lysosomal protease abundantly expressed in the brain. It plays an important role in the regulation of cellular apoptosis. In addition, cathepsin D has been shown to be involved in the pathogenesis of Alzheimer disease and autism. In this study, we developed a novel approach for the preparation of highly purified cathepsin D from the calf brain. This high grade purification is achieved...
Bear serum alpha 2 macroglobulin (α 2 M) was purified by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and partially characterized by tryptic digestion of α 2 M and analysis of the peptides by peptide mass fingerprinting. The molecular weight of bear serum α 2 M was 181 kDa, same as for human serum α 2 M, on SDS-PAGE. However, the MALDI...
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