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A powerful new strategy for the fabrication of high‐density RNA arrays is described. A high‐density DNA array is fabricated by standard photolithographic methods, the surface‐bound DNA molecules are enzymatically copied into their RNA complements from a surface‐bound RNA primer, and the DNA templates are enzymatically destroyed, leaving behind the desired RNA array. The strategy is compatible with...
Gen‐Genie: Der RNA‐vermittelte Genaufbau gelingt ausgehend von Oligonucleotidsequenzen in einem DNA‐Muster innerhalb eines Tages. An jedes oberflächengebundene Oligonucleotid wird ein T7‐Promotor angehängt, und mithilfe von T7‐RNA‐Polymerase werden jeweils viele RNA‐Kopien erzeugt (siehe Bild). Die RNA‐Moleküle lagern sich über Hybridisierung und Ligation zum Volllängen‐Transkript zusammen, das dann...
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