Search results for: E. Neil G. Marsh

Items from 1 to 20 out of 51 results

article

Metal‐dependent assembly of a protein nano‐cage

Ajitha S. Cristie‐David, E. Neil G. Marsh

Short, alpha‐helical coiled coils provide a simple, modular method to direct the assembly of proteins into higher order structures. We previously demonstrated that by genetically fusing de novo–designed coiled coils of the appropriate oligomerization state to a natural trimeric protein, we could direct the assembly of this protein into various geometrical cages. Here, we have extended this approach...

article

Elaborating a coiled‐coil‐assembled octahedral protein cage with additional protein domains

Ajitha S. Cristie‐David, Philipp Koldewey, Ben A. Meinen, James C. A. Bardwell, more

Protein Science > 27 > 11 > 1893 - 1900

De novo design of protein nano‐cages has potential applications in medicine, synthetic biology, and materials science. We recently developed a modular, symmetry‐based strategy for protein assembly in which short, coiled‐coil sequences mediate the assembly of a protein building block into a cage. The geometry of the cage is specified by the combination of rotational symmetries associated with the coiled‐coil...

article

Immobilization of enzyme on a polymer surface

Lei Shen, Kenneth Chun Kuen Cheng, McKenna Schroeder, Pei Yang, more

Surface Science > 2016 > 648 > C > 53-59

We successfully immobilized enzymes onto polymer surfaces via covalent bonds between cysteine groups of the enzyme and dibromomaleimide functionalities present at the polymer surface. In this work, we used nitroreductase (NfsB) as a model enzyme molecule. The polymers were prepared by chemical vapor deposition (CVD) polymerization, resulting in surfaces with dibromomaleimide groups. NfsB variants...

article

Recent progress in hydrocarbon biofuel synthesis: Pathways and enzymes

Feng-Ming Lin, E. Neil G. Marsh, Xiaoxia Nina Lin

Chinese Chemical Letters > 2015 > 26 > 4 > 431-434

Biofuels derived from hydrocarbon biosynthetic pathways have attracted increasing attention. Routes to hydrocarbon biofuels are emerging and mainly fall into two categories based on the metabolic pathways utilized: Fatty acid pathway-based alkanes/alkenes and isoprenoid biosynthetic pathway based terpenes. The primary focus of this review is on recent progress in the application of hydrocarbon biosynthetic...

article

Folate binding protein—Outlook for drug delivery applications

Rachel L. Merzel, Jun-Jie Chen, E. Neil G. Marsh, Mark M. Banaszak Holl

Chinese Chemical Letters > 2015 > 26 > 4 > 426-430

Serum proteins represent an important class of drug and imaging agent delivery vectors. In this mini-review, key advantages of using serum proteins are discussed, followed by the particular advantages and challenges associated with employing soluble folate binding protein. In particular, approaches employing drugs that target folate metabolism are reviewed. Additionally, the slow-onset, tight-binding...

article

Aldehyde‐forming fatty acyl‐CoA reductase from cyanobacteria: expression, purification and characterization of the recombinant enzyme

Fengming Lin, Debasis Das, Xiaoxia N. Lin, E. Neil G. Marsh

The FEBS Journal > 280 > 19 > 4773 - 4781

Long‐chain acyl‐CoA reductases (ACRs) catalyze a key step in the biosynthesis of hydrocarbon waxes. As such they are attractive as components in engineered metabolic pathways for ‘drop in’ biofuels. Most ACR enzymes are integral membrane proteins, but a cytosolic ACR was recently discovered in cyanobacteria. The ACR from Synechococcus elongatus was overexpressed in Escherichia coli, purified and characterized...

article

Perfluoro‐tert‐butyl‐homoserine as a sensitive ¹⁹F NMR reporter for peptide–membrane interactions in solution

Benjamin C. Buer, Benjamin J. Levin, E. Neil G. Marsh

Journal of Peptide Science > 19 > 5 > 308 - 314

Fluorine (¹⁹F) NMR is a valuable tool for studying dynamic biological processes. However, increasing the sensitivity of fluorinated reporter molecules is a key to reducing acquisition times and accessing transient biological interactions. Here, we evaluate the utility a novel amino acid, l‐O‐(perfluoro‐t‐butyl)‐homoserine (pFtBSer), that can easily be synthesized and incorporated into peptides and...

article

Adenosylcobalamin enzymes: Theory and experiment begin to converge

E. Neil G. Marsh, Gabriel D. Román Meléndez

BBA - Proteins and Proteomics > 2012 > 1824 > 11 > 1154-1164

Adenosylcobalamin (coenzyme B ₁₂ ) serves as the cofactor for a group of enzymes that catalyze unusual rearrangement or elimination reactions. The role of the cofactor as the initiator of reactive free radicals needed for these reactions is well established. Less clear is how these enzymes activate the coenzyme towards homolysis and control the radicals once generated. The availability of...

article

Corrigendum: Oxygen‐Independent Decarbonylation of Aldehydes by Cyanobacterial Aldehyde Decarbonylase: A New Reaction of Diiron Enzymes

Debasis Das, Bekir E. Eser, Jaehong Han, Aaron Sciore, more

Angewandte Chemie International Edition > 51 > 32 > 7881 - 7881

article

Berichtigung: Oxygen‐Independent Decarbonylation of Aldehydes by Cyanobacterial Aldehyde Decarbonylase: A New Reaction of Diiron Enzymes

Debasis Das, Bekir E. Eser, Jaehong Han, Aaron Sciore, more

Angewandte Chemie > 124 > 32 > 8004 - 8004

article

Oxygen-Independent Alkane Formation by Non-Heme Iron-Dependent Cyanobacterial Aldehyde Decarbonylase: Investigation of Kinetics and Requirement for an External Electron Donor

Bekir E. Eser, Debasis Das, Jaehong Han, Patrik R. Jones, more

Biochemistry > 2011 > 50 > 49 > 10743 - 10750

article

Oxygen‐Independent Decarbonylation of Aldehydes by Cyanobacterial Aldehyde Decarbonylase: A New Reaction of Diiron Enzymes

Debasis Das, Bekir E. Eser, Jaehong Han, Aaron Sciore, more

Angewandte Chemie > 123 > 31 > 7286 - 7290

Einfach Wasser zugeben: Mit ihrer Struktur gehören cyanobakterielle Aldehyd‐Decarbonylasen zur Enzymfamilie der Nichthäm‐Dieisen‐Oxygenasen – allerdings katalysiert das Enzym, in einer Sauerstoff‐unabhängigen Reaktion, die Hydrolyse von aliphatischen Aldehyden zu Alkanen und Formiat (siehe Schema). Die ungewöhnliche und chemisch schwierige Reaktion läuft höchstwahrscheinlich unter Beteiligung freier...

article

Oxygen‐Independent Decarbonylation of Aldehydes by Cyanobacterial Aldehyde Decarbonylase: A New Reaction of Diiron Enzymes

Debasis Das, Bekir E. Eser, Jaehong Han, Aaron Sciore, more

Angewandte Chemie International Edition > 50 > 31 > 7148 - 7152

Just add water: Structurally, cyanobacterial aldehyde decarbonylases are members of the non‐heme diiron oxygenase family of enzymes. However, the enzyme catalyzes the hydrolysis of aliphatic aldehydes to alkanes and formate (see scheme), in an oxygen‐independent reaction. This unusual and chemically difficult reaction most likely involves free radical intermediates.

article

Using Fluorine Nuclear Magnetic Resonance To Probe Changes in the Structure and Dynamics of Membrane-Active Peptides Interacting with Lipid Bilayers

Yuta Suzuki, Benjamin C. Buer, Hashim M. Al-Hashimi, E. Neil G. Marsh

Biochemistry > 2011 > 50 > 27 > 5979 - 5987

article

Using Fluorine Nuclear Magnetic Resonance To Probe the Interaction of Membrane-Active Peptides with the Lipid Bilayer

Benjamin C. Buer, Jeetender Chugh, Hashim M. Al-Hashimi, E. Neil G. Marsh

Biochemistry > 2010 > 49 > 27 > 5760 - 5765

article

Role of Zinc in Human Islet Amyloid Polypeptide Aggregation

Jeffrey R. Brender, Kevin Hartman, Ravi Prakash Reddy Nanga, Nataliya Popovych, more

Journal of the American Chemical Society > 2010 > 132 > 26 > 8973 - 8983

article

Conversion of (3 S ,4 R )-Tetrahydrodaidzein to (3 S )-Equol by THD Reductase: Proposed Mechanism Involving a Radical Intermediate

Mihyang Kim, E. Neil G. Marsh, Soo-Un Kim, Jaehong Han

Biochemistry > 2010 > 49 > 26 > 5582 - 5587

article

Hydrogen Tunneling in Adenosylcobalamin-Dependent Glutamate Mutase: Evidence from Intrinsic Kinetic Isotope Effects Measured by Intramolecular Competition

Miri Yoon, Hangtian Song, Kristina Håkansson, E. Neil G. Marsh

Biochemistry > 2010 > 49 > 14 > 3168 - 3173

article

Engineering Protein Stability and Specificity Using Fluorous Amino Acids: The Importance of Packing Effects

Benjamin C. Buer, Roberto de la Salud-Bea, Hashim M. Al Hashimi, E. Neil G. Marsh

Biochemistry > 2009 > 48 > 45 > 10810 - 10817

article

Subunit Structure of Benzylsuccinate Synthase ^†

Lei Li, Dustin P. Patterson, Christel C. Fox, Brian Lin, more

Biochemistry > 2009 > 48 > 6 > 1284 - 1292

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English (51)
German (1)

Keywords

BIOFUELS (3)
COENZYME B12 (3)
GLUTAMATE MUTASE (3)
METALLOPROTEINS (3)
COENZYME B 1 2 (2)
BIOBRENNSTOFFE (2)
COBALAMIN-DEPENDENT ENZYME (2)
COILED COILS (2)
ENZYME (2)
ENZYME MECHANISM (2)
FREE RADICAL (2)
FREE RADICALS (2)
FREIE RADIKALE (2)
HYDROLYSEREAKTIONEN (2)
HYDROLYSIS REACTIONS (2)
ISOTOPE EFFECT (2)
METALLOPROTEINE (2)
PROTEIN DESIGN (2)
PROTEIN NANO‐CAGES (2)
RADICAL TRAPS (2)
RADIKALFÄNGER (2)
SEQUENCE HOMOLOGY (2)
COENZYME B 1 2 ; LIGAND BINDING; NUCLEOTIDE; PROTEIN NMR SPECTROSCOPY; PROTEIN FOLDING (1)
ADENOSYLCOBALAMIN (1)
ADOCBL (1)
ALDEHYDE FORMATION (1)
ANTIMICROBIAL PEPTIDE (1)
BIOFUEL (1)
CHEMICAL VAPOR DEPOSITION (1)
COENZYME-B12 (1)
COMPUTATIONAL MODELING (1)
CYANOBACTERIA (1)
DRUG DELIVERY (1)
ENZYMES (1)
FATTY ACID BIOSYNTHESIS PATHWAY (1)
FLUORINATED PROTEIN (1)
FLUORINE NMR (1)
FOLATE BINDING PROTEIN (1)
FOLATE CONJUGATE (1)
FOLIC ACID (1)
FREE ENERGY PROFILE (1)
HETERONUCLEAR NMR (1)
HOMOLYSIS (1)
HYDROCARBON METABOLISM (1)
ISOMERIZATION (1)
ISOPRENOID BIOSYNTHESIS PATHWAY (1)
ISOTOPE EFFECTS (1)
ISOTOPE PARTITIONING (1)
LABELED MOLECULES (1)
METABOLIC ENGINEERING (1)
MSI‐78 (1)
MUTAGENESIS (1)
MUTASE (1)
ORGANIC RADICALS (1)
PROTEIN DYNAMICS (1)
PROTEIN STRUCTURE (1)
PROTEIN-RADICAL (1)
S-ADENOSYLMETHIONINE (1)
SERUM PROTEIN (1)
SUM FREQUENCY GENERATION VIBRATIONAL SPECTROSCOPY (1)
SURFACE IMMOBILIZED ENZYMES (1)
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Data set

ACS (30)
Elsevier (13)
Wiley (8)

Journal

Biochemistry (20)
Journal of the American Chemical Society (7)
Angewandte Chemie (2)
Angewandte Chemie International Edition (2)
Chinese Chemical Letters (2)
FEBS Letters (2)
Protein Science (2)
Archives of Biochemistry and Biophysics (1)
BBA - Proteins and Proteomics (1)
Bioorganic Chemistry (1)
Chemistry & Biology (1)
Crystal Growth & Design (1)
Current Opinion in Chemical Biology (1)
Journal of Molecular Biology (1)
Journal of Peptide Science (1)
Organic Letters (1)
Structure (1)
Surface Science (1)
Tetrahedron (1)
The FEBS Journal (1)
The Journal of Physical Chemistry B (1)
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INFONA - science communication portal

Search results for: E. Neil G. Marsh

Metal‐dependent assembly of a protein nano‐cage

Elaborating a coiled‐coil‐assembled octahedral protein cage with additional protein domains

Immobilization of enzyme on a polymer surface

Recent progress in hydrocarbon biofuel synthesis: Pathways and enzymes

Folate binding protein—Outlook for drug delivery applications

Aldehyde‐forming fatty acyl‐CoA reductase from cyanobacteria: expression, purification and characterization of the recombinant enzyme

Perfluoro‐tert‐butyl‐homoserine as a sensitive ¹⁹F NMR reporter for peptide–membrane interactions in solution

Adenosylcobalamin enzymes: Theory and experiment begin to converge

Corrigendum: Oxygen‐Independent Decarbonylation of Aldehydes by Cyanobacterial Aldehyde Decarbonylase: A New Reaction of Diiron Enzymes

Berichtigung: Oxygen‐Independent Decarbonylation of Aldehydes by Cyanobacterial Aldehyde Decarbonylase: A New Reaction of Diiron Enzymes

Oxygen-Independent Alkane Formation by Non-Heme Iron-Dependent Cyanobacterial Aldehyde Decarbonylase: Investigation of Kinetics and Requirement for an External Electron Donor

Oxygen‐Independent Decarbonylation of Aldehydes by Cyanobacterial Aldehyde Decarbonylase: A New Reaction of Diiron Enzymes

Oxygen‐Independent Decarbonylation of Aldehydes by Cyanobacterial Aldehyde Decarbonylase: A New Reaction of Diiron Enzymes

Using Fluorine Nuclear Magnetic Resonance To Probe Changes in the Structure and Dynamics of Membrane-Active Peptides Interacting with Lipid Bilayers

Using Fluorine Nuclear Magnetic Resonance To Probe the Interaction of Membrane-Active Peptides with the Lipid Bilayer

Role of Zinc in Human Islet Amyloid Polypeptide Aggregation

Conversion of (3 S ,4 R )-Tetrahydrodaidzein to (3 S )-Equol by THD Reductase: Proposed Mechanism Involving a Radical Intermediate

Hydrogen Tunneling in Adenosylcobalamin-Dependent Glutamate Mutase: Evidence from Intrinsic Kinetic Isotope Effects Measured by Intramolecular Competition

Engineering Protein Stability and Specificity Using Fluorous Amino Acids: The Importance of Packing Effects

Subunit Structure of Benzylsuccinate Synthase ^†

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